First report of blaVEB-3 and blaKPC-2 coexistence with a novel blaKPC-2 transposon in Klebsiella michiganensis

Infection Genetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 105740 - 105740

Published: March 1, 2025

Klebsiella michiganensis, an emerging opportunistic pathogen, poses public health risks due to its increasing multidrug resistance (MDR), especially carbapenems. A 46-year-old man with pulmonary fibrosis was hospitalized in Guangzhou, China, for worsening pneumonia. multidrug-resistant K. michiganensis strain (YK6) isolated from his sputum before treatment. The characterized using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing (AST), and whole genome sequencing (WGS). Targeted therapy guided by AST successfully resolved the infection. YK6 exhibited carbapenems, β-lactam/β-lactamase inhibitors, cephalosporins, aminoglycosides, quinolones, except colistin tigecycline. Genomic analysis revealed a 41.9-kb MDR island intact I-E CRISPR-Cas system on chromosome, along two plasmids: IncFIA/IncFII plasmid pYK6-1 carrying blaKPC-2 IncC pYK6-2 harboring blaVEB-3. novel blaKPC-2-transposon identified, consisting of non-Tn4401 element (NTE)-like structure (Tn3-ISKpn27-blaKPC-2-ΔISKpn6-korC) flanked inversely oriented ISKpn19-tnpM-tnpR elements 31-bp inverted repeats never reported, configuration did not reported previously. Furthermore, blaVEB-3 genetic environment featured unique cassette: IS26-IS6100-blaVEB-3-tnp-ISAs1-qacEΔ1-sul1-ISCR1. An additional ISAs1 insertion between tnpF-like integrase qacEΔ1 distinguishes it similar blaVEB-3-harboring cassettes. region likely originated homologous recombination mediated IS26 Tn5403, which flank gene cassette. To our knowledge, this is first report concurrent transposon structure. These findings highlight urgent need enhanced surveillance prevent treatment failures.

Language: Английский

Coexistence of a nonresistance-conferring IncI1 plasmid favors persistence of the bla _CTX-M -bearing IncFII plasmid in Escherichia coli

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(6)

Published: April 30, 2024

The interaction between coexisting plasmids can affect plasmid-carried resistance gene persistence and spread. However, whether the of

Language: Английский