Steviol rebaudiosides bind to four different sites of the human sweet taste receptor (T1R2/T1R3) complex explaining confusing experiments
Communications Chemistry,
Journal Year:
2024,
Volume and Issue:
7(1)
Published: Oct. 18, 2024
Sucrose
provides
both
sweetness
and
energy
by
binding
to
Venus
flytrap
domains
(VFD)
of
the
heterodimeric
sweet
taste
receptor
(T1R2/T1R3).
In
contrast,
non-caloric
sweeteners
such
as
sucralose
aspartame
only
bind
one
specific
domain
(VFD2)
T1R2,
resulting
in
high-intensity
sweetness.
this
study,
we
investigate
mechanism
various
steviol
glycosides,
artificial
sweeteners,
a
negative
allosteric
modulator
(lactisole)
at
four
distinct
sites:
VFD2,
VFD3,
transmembrane
2
(TMD2),
TMD3
through
experiments
computational
docking
studies.
Our
results
reveal
multiple
sites
for
tested
ligands,
including
radiolabeled
ligands.
experimental
evidence
demonstrates
that
C20
carboxy
terminus
Gα
protein
can
intracellular
region
either
TMD2
or
TMD3,
altering
GPCR
affinity
high-affinity
state
glycosides.
These
findings
provide
mechanistic
understanding
structure
function
receptor.
other
different
(T1R2/T1R3),
levels
Here,
authors
T1R2/T1R3
studies,
revealing
ligands
structural–
correlations
ligand–receptor
interactions.
Language: Английский
Optical biosensors for diagnosing neurodegenerative diseases
Khuong Duy Mac,
No information about this author
Judith Su
No information about this author
Deleted Journal,
Journal Year:
2025,
Volume and Issue:
2(1)
Published: May 2, 2025
Neurodegenerative
diseases
involve
the
progressive
loss
of
neurons
in
brain
and
nervous
system,
leading
to
functional
decline.
Early
detection
is
critical
for
improving
outcomes
advancing
therapies.
Optical
biosensors,
some
which
offer
rapid,
label-free,
ultra-sensitive
detection,
have
been
applied
early
diagnosis
drug
screening.
This
review
examines
principles
performance
different
optical
biosensors
used
diagnosing
neurodegenerative
discusses
potential
future
advancements.
Language: Английский
Coupled Nanomechanical Resonator with Protein-Interaction Vibration for an Ultrasensitive Label-Free Biosensor
ACS Applied Materials & Interfaces,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 2, 2025
A
mechanical-resonator
biosensor
detects
target
molecules
attached
to
the
resonator
surface
through
a
change
in
resonant
frequency
caused
by
mass-loading
effect.
Since
mass-detection
sensitivity
can
be
improved
thinning
thickness,
much
effort
has
been
devoted
development
of
thinner
resonator.
Here,
we
propose
more
effective
strategy
for
an
ultrasensitive
biosensor.
When
is
close
local
vibration
at
specific
interaction
between
and
receptor
(∼30
GHz),
significantly
large
occur
because
energy
coupling
target-receptor
(similar
avoided
crossing).
For
realizing
this
strategy,
developed
ultrahigh-frequency
nanomechanical
multilayer
graphene.
The
resonator-frequency
near
avoided-crossing
about
10
times
higher
than
that
effect,
which
allowed
label-free
detection
C-reactive
protein
with
limit
pg/mL
or
less
even
serum.
Language: Английский
Whispering gallery mode optical resonators for biological and chemical detection: current practices, future perspectives, and challenges
Reports on Progress in Physics,
Journal Year:
2024,
Volume and Issue:
88(1), P. 016402 - 016402
Published: Dec. 3, 2024
Abstract
Sensors
are
important
for
a
wide
variety
of
applications
include
medical
diagnostics
and
environmental
monitoring.
Due
to
their
long
photon
confinement
times,
whispering
gallery
mode
(WGM)
sensors
among
the
most
sensitive
currently
in
existence.
We
briefly
discuss
what
WGM
sensors,
principles
sensing,
history
field,
beginning
with
Mie
theory.
recent
work
field
on
using
these
resonators
as
focusing
particularly
biological
chemical
sensing
applications.
how
sensorgrams
acquired
fundamental
measurement
limits.
In
addition,
we
interpret
binding
curves
extract
physical
parameters
such
affinity
constants.
controversy
surrounding
single-molecule
detection
hybrid
nanoparticle
sensors.
place
context
others
technologies
both
labeled
label-free.
Finally,
believe
promising
devices,
outline
remaining
challenges,
provide
an
outlook
future.
Language: Английский