Somatostatin-type and allatostatin-C–type neuropeptides are paralogous and have opposing myoregulatory roles in an echinoderm DOI Creative Commons
Ya Zhang, Luis Alfonso Yañez Guerra, Ana B. Tinoco

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(7)

Published: Feb. 10, 2022

Somatostatin (SS) and allatostatin-C (ASTC) are inhibitory neuropeptides in chordates protostomes, respectively, which hitherto were identified as orthologs. However, echinoderms have two SS/ASTC-type (SS1 SS2), here, our analysis of sequence data indicates that SS1 is an ortholog ASTC SS2 SS. The occurrence both SS-type ASTC-type provides a unique context to compare their physiological roles. Investigation the expression actions neuropeptide ArSS1 starfish Asterias rubens revealed it causes muscle contraction (myoexcitation), contrasting with myoinhibitory effects ArSS2. Our findings suggest paralogous originated by gene duplication common ancestor Bilateria, only one type being retained protostomes but types echinoderms. Loss may been due functional redundancy regulators processes. Conversely, retention be consequence evolution myoexcitatory role for mediated yet unknown signaling mechanisms.

Language: Английский

Calcitonin receptors are ancient modulators for rhythms of preferential temperature in insects and body temperature in mammals DOI Open Access
Tadahiro Goda, Masao Doi,

Yujiro Umezaki

et al.

Genes & Development, Journal Year: 2018, Volume and Issue: 32(2), P. 140 - 155

Published: Jan. 15, 2018

Daily body temperature rhythm (BTR) is essential for maintaining homeostasis. BTR regulated separately from locomotor activity rhythms, but its molecular basis largely unknown. While mammals internally regulate BTR, ectotherms, including Drosophila , exhibit preference (TPR) behavior to BTR. Here, we demonstrate that the diuretic hormone 31 receptor (DH31R) mediates TPR during active phase in . DH31R expressed clock cells, and ligand, DH31, acts on cells phase. Surprisingly, mouse homolog of DH31R, calcitonin (Calcr), suprachiasmatic nucleus (SCN) fluctuations mice. Importantly, Calcr are not required coordinating rhythms. Our results represent first evidence distinctly rhythms show DH31R/Calcr an ancient specific mediator organisms ranging ectotherms endotherms.

Language: Английский

Citations

42
Functional Characterization of Paralogous Gonadotropin-Releasing Hormone-Type and Corazonin-Type Neuropeptides in an Echinoderm DOI Creative Commons

Shi Tian,

Michaela Egertová,

Maurice R. Elphick

et al.

Frontiers in Endocrinology, Journal Year: 2017, Volume and Issue: 8

Published: Sept. 29, 2017

Homologs of the vertebrate neuropeptide gonadotropin-releasing hormone (GnRH) have been identified in invertebrates, including insect corazonin (CRZ). Recently, we reported discovery GnRH-type and CRZ-type signaling systems an echinoderm, starfish Asterias rubens, demonstrating that evolutionary origin paralogous neuropeptides can be traced back to common ancestor protostomes deuterostomes. Here, investigated physiological roles (ArGnRH) (ArCRZ) A. using mRNA situ hybridization, immunohistochemistry vitro pharmacology. ArGnRH precursor (ArGnRHP)-expressing cells ArGnRH-immunoreactive and/or processes are present radial nerve cords, circumoral ring, digestive system (e.g., cardiac stomach pyloric stomach), body wall-associated muscle (apical muscle), appendages (tube feet, terminal tentacle). The general distribution ArCRZ (ArCRZP)-expressing is similar ArGnRHP, but with specific local differences. For example, expressing ArGnRHP both ectoneural hyponeural regions cords whereas ArCRZP were only observed region. In pharmacological experiments revealed cause contraction stomach, apical muscle, tube foot preparations. However, was more potent/effective than as a contractant muscle. These findings demonstrate myoexcitatory starfish, differences their expression patterns activities indicative distinct roles. This first study investigate deuterostome, providing new insights into evolution comparative physiology these Bilateria.

Language: Английский

Citations

41
Echinoderms provide missing link in the evolution of PrRP/sNPF-type neuropeptide signalling DOI Creative Commons
Luis Alfonso Yañez Guerra, Xingxing Zhong, Ismail Moghul

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: June 24, 2020

Neuropeptide signalling systems comprising peptide ligands and cognate receptors are evolutionarily ancient regulators of physiology behaviour. However, there challenges associated with determination orthology between neuropeptides in different taxa. Orthologs vertebrate neuropeptide-Y (NPY) known as neuropeptide-F (NPF) have been identified protostome invertebrates, whilst prolactin-releasing (PrRP) short (sNPF) paralogs NPY/NPF vertebrates protostomes, respectively. Here we investigated the occurrence NPY/NPF/PrRP/sNPF-related a deuterostome invertebrate phylum - Echinodermata. Analysis transcriptome/genome sequence data revealed loss NPY/NPF-type signalling, but orthologs PrRP-type sNPF/PrRP-type were echinoderms. Furthermore, experimental studies that neuropeptide pQDRSKAMQAERTGQLRRLNPRF-NH2 is potent ligand for receptor starfish Asterias rubens. Our findings indicate sNPF-type orthologous originated paralog Urbilateria.

Language: Английский

Citations

37
Invertebrate Gonadotropin-Releasing Hormone Receptor Signaling and Its Relevant Biological Actions DOI Open Access

Tsubasa Sakai,

Tatsuya Yamamoto, Shin Matsubara

et al.

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(22), P. 8544 - 8544

Published: Nov. 12, 2020

Gonadotropin-releasing hormones (GnRHs) play pivotal roles in reproduction via the hypothalamus-pituitary-gonad axis (HPG axis) vertebrates. GnRHs and their receptors (GnRHRs) are also conserved invertebrates lacking HPG axis, indicating that invertebrate do not serve as "gonadotropin-releasing factors" but, rather, function neuropeptides directly regulate target tissues. All vertebrate urochordate comprise 10 amino acids, whereas amphioxus, echinoderm, protostome GnRH-like peptides 11- or 12-residue peptides. Intracellular calcium mobilization is major second messenger for GnRH signaling cephalochordates, echinoderms, protostomes, while GnRHRs stimulate cAMP production pathways. Moreover, ligand-specific modulation of signal transduction heterodimerization between GnRHR paralogs indicates species-specific evolution Ciona intestinalis. The characterization authentic putative various tissues vitro vivo activities indicate responsible regulation both reproductive nonreproductive functions. In this review, we examine our current understanding perspectives on primary sequences, tissue distribution mRNA expression, transduction, biological functions receptors.

Language: Английский

Citations

36
Somatostatin-type and allatostatin-C–type neuropeptides are paralogous and have opposing myoregulatory roles in an echinoderm DOI Creative Commons
Ya Zhang, Luis Alfonso Yañez Guerra, Ana B. Tinoco

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(7)

Published: Feb. 10, 2022

Somatostatin (SS) and allatostatin-C (ASTC) are inhibitory neuropeptides in chordates protostomes, respectively, which hitherto were identified as orthologs. However, echinoderms have two SS/ASTC-type (SS1 SS2), here, our analysis of sequence data indicates that SS1 is an ortholog ASTC SS2 SS. The occurrence both SS-type ASTC-type provides a unique context to compare their physiological roles. Investigation the expression actions neuropeptide ArSS1 starfish Asterias rubens revealed it causes muscle contraction (myoexcitation), contrasting with myoinhibitory effects ArSS2. Our findings suggest paralogous originated by gene duplication common ancestor Bilateria, only one type being retained protostomes but types echinoderms. Loss may been due functional redundancy regulators processes. Conversely, retention be consequence evolution myoexcitatory role for mediated yet unknown signaling mechanisms.

Language: Английский

Citations

20