Electrophoresis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 6, 2024
ABSTRACT
The
dynamics
of
three
one‐step
focusing
protocols
described
in
the
literature
for
IEF‐MS
analyses
proteins
are
assessed
by
computer
simulation.
Focusing
101
carrier
ampholytes
(pI
range
3.0–11.0)
and
9
analytes
3.9–10.4)
with
concurrent
electrophoretic
mobilization
an
electroosmosis‐free
environment
is
studied
via
use
following:
(1)
acid
as
anolyte
catholyte,
(2)
a
plug
base
between
sample
(3)
incorporated
into
acid‐based
catholyte.
data
reveal
that
first
approach
limited
applicability
separation
efficiency
it
provides
separated
transient
foci
acidic
only.
second
system
largely
extends
range,
separation,
sensitivity
can
be
applied
to
pIs
high
10.
same
true
Dynamic
simulation
time
insight
complexity
these
systems
buffer
components
compatible
MS
detection.
Simulation
attractive
simple
tool
characterization
involved
processes
assay
optimization.
Frontiers in Lab on a Chip Technologies,
Journal Year:
2022,
Volume and Issue:
1
Published: Dec. 8, 2022
During
the
recent
pandemic
outbreak,
Lab-on-Chip
devices
did
not
manage
to
fully
reach
their
potential
in
rapid
diagnosis
of
pathogens,
mainly
due
lack
cost-effective
LoC
solutions
integrated
with
all
required
sample
preparation
modules.
This
paper
presents
such
a
critical
step,
aiming
translate
electrochemical
pH
control
into
practical
protein
preconcentration
modules,
easy
integrate
subsequent
quantification
modules
seamlessly
via
Lab-on-PCB
technology.
In
this
work
we
present
device
capable
electrochemically
controlling
solution
local
an
individually
addressed
electrode
PCB
array.
The
electrodes
were
functionalised
electropolymerised
self-assembled
monolayer
4-Aminothiophenol
and
subjected
voltages
0.2–0.4
V,
evaluating
for
first
time
bias
effect
both
over
space.
study
enables
implementation
technique
as
isoelectric
focusing,
informed
design
arrays
appropriate
size
spacing.
Green Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
10, P. 100122 - 100122
Published: June 25, 2024
This
article
has
been
retracted:
Please
see
Elsevier
Policy
on
Article
Withdrawal
(https://www.elsevier.com/locate/withdrawalpolicy).
retracted
at
the
request
of
authors.
The
manuscript
was
submitted
before
a
final
round
revision
by
As
result,
not
all
authors
had
opportunity
to
review
and
approve
it,
there
are
series
errors
that
should
have
rectified
prior
publication.
These
are:
•
Some
figures
do
contain
reference
original
source
(permissions
obtained
reproduce
but
this
properly
represented).
sentences
in
Introduction
were
taken
verbatim
from
other
sources
while
no
included
source.
need
further
supplement
some
data
discussion
improve
paper.
apologise
for
error.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 30, 2024
Methods
for
the
reliable
and
effective
detection
identification
of
impurities
are
crucial
to
ensure
quality
safety
biopharmaceutical
products.
Technical
limitations
constrain
accurate
individual
impurity
peaks
by
size-based
electrophoresis
separations
followed
mass
spectrometry.
This
study
presents
a
electrophoretic
method
detecting
identifying
in
antibody
production.
A
hydrogen
sulfide-accelerated
degradation
was
employed
generate
known
products
observed
bioreactors
that
forms
basis
size
calibration.
LabChip
GXII
channel
enabled
rapid
(<
1
min)
based
on
size,
while
capillary
zone
electrophoresis-mass
spectrometry
(CZE-MS)
facilitated
their
identification.
We
combine
these
techniques
examine
resulting
from
cell
culture
harvest
conditions
forced
assess
stability.
To
mimic
impact
degradation,
we
subjected
samples
cathepsin
at
different
pH
buffers
or
exposed
them
high
temperature.
Our
demonstrated
feasibility
broad
applicability
using
CZE-MS
generated
spectral
library
unambiguously
assign
throughput
(i.e.,
GXII)
with
identifications
likely
impurity.
Overall,
this
strategy
combines
utility
as
high-resolution
separation
methods
typically
used
detect
(not
identify)
during
discovery
development
therapeutics.
Electrophoresis,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 6, 2024
ABSTRACT
The
dynamics
of
three
one‐step
focusing
protocols
described
in
the
literature
for
IEF‐MS
analyses
proteins
are
assessed
by
computer
simulation.
Focusing
101
carrier
ampholytes
(pI
range
3.0–11.0)
and
9
analytes
3.9–10.4)
with
concurrent
electrophoretic
mobilization
an
electroosmosis‐free
environment
is
studied
via
use
following:
(1)
acid
as
anolyte
catholyte,
(2)
a
plug
base
between
sample
(3)
incorporated
into
acid‐based
catholyte.
data
reveal
that
first
approach
limited
applicability
separation
efficiency
it
provides
separated
transient
foci
acidic
only.
second
system
largely
extends
range,
separation,
sensitivity
can
be
applied
to
pIs
high
10.
same
true
Dynamic
simulation
time
insight
complexity
these
systems
buffer
components
compatible
MS
detection.
Simulation
attractive
simple
tool
characterization
involved
processes
assay
optimization.
