Preparation, identification and molecular docking of two novel anti-aging peptides from perilla seed DOI Creative Commons
Lingling Wang, Liping Qu, Bingwei He

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33604 - e33604

Published: June 25, 2024

Perilla seed meal is an important agricultural by-product of perilla oil extraction. The antioxidant and anti-aging activities protein hydrolysate were investigated, the bioactive peptides identified to maximize utilization resources. Anti-aging using a combination peptidomics in silico bioinformatics. Furthermore, potential molecular mechanism these was explored through docking RT-PCR. results showed significant properties F2 (MW 3 kDa ∼5 kDa) by inhibition reactive oxygen species (ROS) production β-galactosidase activity. Nine novel from subsequently synthesized explore their bioactivities. two peptides, NFF PMR, found promote proliferation keratinocytes (HaCaT cells) suppress level ROS activity β-galactosidase. Both exhibited strong binding affinity with Keap1 protein. Additionally, PMR downregulated expression matrix metalloproteinases (MMPs) degradation collagens (COLs). underlying might involve competitive facilitate release Nrf2 activation NF-κB signal pathway. This study provides theoretical basis for application functional cosmetics presents perspective investigation additional food-derived peptides.

Language: Английский

Advance in peptide-based drug development: delivery platforms, therapeutics and vaccines DOI Creative Commons
Wen‐Jing Xiao, Wenjie Jiang, Zheng Chen

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 5, 2025

The successful approval of peptide-based drugs can be attributed to a collaborative effort across multiple disciplines. integration novel drug design and synthesis techniques, display library technology, delivery systems, bioengineering advancements, artificial intelligence have significantly expedited the development groundbreaking drugs, effectively addressing obstacles associated with their character, such as rapid clearance degradation, necessitating subcutaneous injection leading increasing patient discomfort, ultimately advancing translational research efforts. Peptides are presently employed in management diagnosis diverse array medical conditions, diabetes mellitus, weight loss, oncology, rare diseases, additionally garnering interest facilitating targeted platforms advancement vaccines. This paper provides an overview present market clinical trial progress therapeutics, platforms, It examines key areas through literature analysis emphasizes structural modification principles well recent advancements screening, design, technologies. accelerated including peptide-drug complexes, new vaccines, innovative diagnostic reagents, has potential promote era precise customization disease therapeutic schedule.

Language: Английский

Citations

1
From green innovations in oligopeptide to oligonucleotide sustainable synthesis: differences and synergies in TIDES chemistry DOI Creative Commons
Lucia Ferrazzano, Dario Corbisiero, Alessandra Tolomelli

et al.

Green Chemistry, Journal Year: 2023, Volume and Issue: 25(4), P. 1217 - 1236

Published: Jan. 1, 2023

Can the green innovations introduced in peptide synthesis be transferred to oligonucleotide manufacturing? A critical analysis disclose potential of TIDES.

Language: Английский

Citations

21
Mechanochemical Deoxyfluorination of Carboxylic Acids to Acyl Fluorides and Successive Mechanochemical Amide Bond Formation DOI

Zhengyu Zhao,

Sota Ikawa,

Soichiro Mori

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(9), P. 3565 - 3574

Published: Feb. 16, 2024

Diverse methods have been reported for the synthesis of acyl fluorides; however, an environmentally benign method fluorides remains underdeveloped. In this study, we developed solvent-free mechanochemical deoxyfluorination carboxylic acids to mediated by 1,1,2,2-tetrafluoroethyl-N,N-dimethylamine (TFEDMA) using a ball mill. This facilitated high product yields in short reaction times even sterically challenged acids. We also realized coupling and amines as well TFEDMA-mediated direct with via sequential one-pot deoxyfluorination/coupling pathway. Furthermore, protocol was expanded include peptide synthesis. The efficiency protocol, terms speed, characteristics, favorable E-factor, aligns requirements current environmental policies.

Language: Английский

Citations

7
Triethyl phosphate (TEP) as a green solvent for solid-phase peptide synthesis (SPPS) DOI Creative Commons

K. P. Nandhini,

Nosipho Cele, Beatriz G. de la Torre

et al.

Green Chemistry Letters and Reviews, Journal Year: 2024, Volume and Issue: 17(1)

Published: March 21, 2024

Triethyl phosphate (TEP) is a new member of the Green Solid-Phase Peptide Synthesis (GSPPS) toolbox. TEP shows almost no toxicity and carcinogenic or mutagenic properties. It degrades, giving rather innocuous phosphoric acid ethanol. has low viscosity, which makes it appealing for solid-phase peptide synthesis (SPPS), where reagents delivered by solvent should penetrate polymeric matrix. dissolves most Fmoc-amino acids key coupling additives. The use allowed SPPS several model peptides using conventional polystyrene resin at room temperature, while other green solvents required more sophisticated resins and/or elevated temperatures. In conclusion, an excellent contender to become choice.

Language: Английский

Citations

7
Green Solid-Phase Peptide Synthesis: Oxyma-Triggered Spectrophotometric Monitoring of Residual Piperidine DOI Creative Commons

Søren Lindbæk Broman,

Martin Rosenberg, Felix Wojcik

et al.

Organic Process Research & Development, Journal Year: 2024, Volume and Issue: 28(3), P. 666 - 673

Published: Feb. 28, 2024

Solid-phase peptide synthesis (SPPS) is an efficient platform technology for synthesizing synthetic peptides but has environmental downside due to the use of vast amounts toxic solvents. In recent years, a big effort been made replace these solvents with more environmentally benign alternatives; however, this work not had significant effect on reducing PMI and carbon footprint SPPS. Herein, we demonstrate that by adding low concentration Oxyma Pure waste stream after Fmoc-removal, simple real-time online UV–vis monitoring system can quantify piperidine needed sensitivity. addition potential washing volumes inexpensive easy-to-implement process analytical tool allows determination completed visual inspection yellow color in presence piperidine. Moreover, manual sampling needed, which reduces overall time. Importantly, methodology was found be compatible green SPPS performed 10–50% DMSO EtOAc mixtures.

Language: Английский

Citations

6
Cryptic enzymatic assembly of peptides armed with β-lactone warheads DOI Creative Commons
Guangcai Xu, Daniele Torri,

Sebastian Cuesta-Hoyos

et al.

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: 20(10), P. 1371 - 1379

Published: July 1, 2024

Nature has evolved biosynthetic pathways to molecules possessing reactive warheads that inspired the development of many therapeutic agents, including penicillin antibiotics. Peptides armed with electrophilic have proven be particularly effective covalent inhibitors, providing essential antimicrobial, antiviral and anticancer agents. Here we provide a full characterization nature deploys assemble peptides β-lactone warheads, which are potent proteasome inhibitors promising activity. Warhead assembly involves three-step cryptic methylation sequence, is likely required reduce unfavorable electrostatic interactions during sterically demanding β-lactonization. Amide-bond synthetase adenosine triphosphate (ATP)-grasp enzymes couple amino acids warhead, generating bioactive peptide products. After reconstituting entire pathway in vitro, go on deliver diverse range analogs through enzymatic cascade reactions. Our approach more efficient cleaner than synthetic methods currently used produce clinically important warhead-containing peptides.

Language: Английский

Citations

6
Advances in solid-phase peptide synthesis in aqueous media (ASPPS) DOI Creative Commons
Da’san M. M. Jaradat, Othman Al Musaimi, Fernando Alberício

et al.

Green Chemistry, Journal Year: 2022, Volume and Issue: 24(17), P. 6360 - 6372

Published: Jan. 1, 2022

Technologies to enable Aqueous Solid-Phase Peptide Synthesis (ASPPS).

Language: Английский

Citations

25
A Field Guide to Optimizing Peptoid Synthesis DOI Creative Commons
Abigail Mae Clapperton, Jon Babi, Helen Tran

et al.

ACS Polymers Au, Journal Year: 2022, Volume and Issue: 2(6), P. 417 - 429

Published: Sept. 15, 2022

N-Substituted glycines (peptoids) are a class of peptidomimetic molecules used as materials for health, environmental, and drug delivery applications. Automated solid-phase synthesis is the most widely approach preparing polypeptoids, with range published protocols modifications selected synthetic targets. Simultaneously, emerging solution-phase syntheses being leveraged to overcome limitations in access high-molecular weight polypeptoids. This Perspective aims outline strategies optimization both solid- synthesis, provide technical considerations robotic synthesizers, offer an outlook on advances methodologies. The sections explore steps protocol optimization, accessing complex side chains, adaptation synthesizers; cover selection initiators, chain compatibility, controlling polymerization efficiency scale. text acts "field guide" researchers aiming leverage flexibility adaptability peptoids their research.

Language: Английский

Citations

24
Dimethyl carbonate as a green alternative to acetonitrile in reversed-phase liquid chromatography. Part I: Separation of small molecules DOI Creative Commons
Simona Felletti,

Matteo Spedicato,

Desiree Bozza

et al.

Journal of Chromatography A, Journal Year: 2023, Volume and Issue: 1712, P. 464477 - 464477

Published: Oct. 30, 2023

Nowadays, environmental problems are drawing the attention of governments and international organisations, which therefore encouraging transition to green industrial processes approaches. In this context, chemists can help indicate a suitable direction. Beside efforts focused on greening synthetic approaches, currently also analytical techniques separations under observation, especially those employing large volumes organic solvents, such as reversed-phase liquid chromatography (RPLC). Acetonitrile has always been considered best performing modifier for RPLC applications, due its chemical features (complete miscibility in water, UV transparency, low viscosity etc); nevertheless, it suffers severe shortcomings, most importantly, does not fully comply with Environmental, Health Safety (EHS) requirements. For these reasons, alternative greener solvents being investigated, easily available alcohols. work, chromatographic performance common used chromatography, i.e., acetonitrile, ethanol isopropanol, have compared scarcely solvent, dimethyl carbonate (DMC). The analytes interest were two small molecules, caffeine paracetamol, whose kinetics retention behaviour obtained four compared, all contributions band broadening assessed. Results about kinetic very promising, indicating that amount (7 % v/v) DMC is able produce same efficiency 2.5-times larger ACN volume (18 v/v), than This paper reports, first time, fundamental studies concerning mass transfer phenomena when an solvent RPLC, and, together companion paper, represents results research final aim was discover whether applications both linear preparative conditions.

Language: Английский

Citations

16
Peptides, solid-phase synthesis and characterization: Tailor-made methodologies DOI Creative Commons
Fanny Guzmán,

Mónica Aróstica,

Tanya Román

et al.

Electronic Journal of Biotechnology, Journal Year: 2023, Volume and Issue: 64, P. 27 - 33

Published: May 4, 2023

Solid-Phase Peptide Synthesis (SPPS) is a mature technique widely used in research and production. There are different approaches that fulfill the diverse requirements, regarding number, quantity quality of peptides. We have implemented three laboratory protocols synthesis cover these needs. These been tested, results analyzed with two sequences previous works. The peptide such as tea bag, microwave manual allowed obtaining specific yields 8, 43 64% for NBC112 36, 46 78% NBC759 protocols, respectively. Each protocol has application contexts advantages disadvantages each case. allow obtention peptides good purity can be according to needs requirements. This article includes an interactive 360-degree video. To view it correctly, necessary scroll through screen navigate across where you will find 10 points. For immersive experience, head-mounted display used. Please, visit this URL: http://ejbiotechnology.info/public/360view/2023/VTPCARDENAS1v1/index.html. How cite: Guzmán F, Aróstica M, Román T, et al. Peptides, solid-phase characterization: Tailor-made methodologies. Electron J Biotechnol 2023;64. https://doi.org/10.1016/j.ejbt.2023.01.005.

Language: Английский

Citations

14