On-Chip Photoacoustics-Activated Cell Sorting (PA-ACS) for Label-Free and High-Throughput Detection and Screening of Microalgal Cells

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(3), P. 1301 - 1309

Published: Jan. 9, 2024

Microalgae play a crucial role in global carbon cycling as they convert dioxide into various valuable macromolecules. Among them, Haematococcus pluvialis (H. pluvialis) is the richest natural source of astaxanthin (AXT), which antioxidant, anti-inflammatory, and antiapoptosis agent. These benefits make AXT highly commercially pharmaceuticals, cosmetics, nutritional industries. However, intrinsic genetic characteristics extrinsic cultivation conditions influence biomass gains, leading to low productivity extraction main techno-economic bottlenecks this industry. Thus, detecting H. essential determine multiple parameters on biocompound accumulation, enabling optimization enrichment AXT-rich cells. This work developed an opto-acousto-fluidic microplatform for detection, analysis, sorting microalgae. Via label-free monitoring sample-induced ultrasonic signals, photoacoustic microscopic system was proposed provide full-field visualization AXT's content distribution inside When employed on-chip image-based flow cytometry, our can also offer high-throughput measurements intracellular real time, demonstrates similar results conventional spectrophotometry methods further reveals heterogeneity at single-cell level. In addition, solenoid valve-pump dual-mode cell sorter integrated effective cells with maximum working frequency 0.77 Hz, reducing fluid response time by 50% rising 40-fold recovery. The have more accumulation (>30 μm diameter) were 4.38-fold enriched almost no dead empty small green According results, automated reliable photoacoustics-activated (PA-ACS) screen remove impurities terminal stage cultivation, thereby increasing effectiveness purity extraction. be adopted enrich strains mutants production biofuels or other rare organic substances such β-carotene lutein.

Language: Английский

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Spiral Large-Dimension Microfluidic Channel for Flow-Rate- and Particle-Size-Insensitive Focusing by the Stabilization and Acceleration of Secondary Flow

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(4), P. 1750 - 1758

Published: Jan. 12, 2024

Inertial microfluidics has demonstrated its ability to focus particles in a passive and straightforward manner. However, achieving flow-rate- particle-size-insensitive focusing large-dimension channels with simple design remains challenging. In this study, we developed spiral microfluidic channel achieve inertial focusing. By designing unique "big buffering area" "small the microchannel, observed stabilization acceleration of secondary flow. Our optimized allowed for efficient (>99.9%) 15 μm within wide range flow rates (0.5-4.5 mL/min) during long operation duration (0-60 min). Additionally, achieved effective (>95%) different-sized (7, 10, 15, 30 μm) three types tumor cells (K562, HeLa, MCF-7) near inner wall 1 mm outlet when applying different (1-3 mL/min). Finally, successful 3D cell was an device, positioned at distance 50 from wall. strategy stabilizing accelerating Dean-like through configuration proved be highly that is insensitive rate particle size, particularly channels. Consequently, it shows great potential use hand-operated tools cytometry.

Language: Английский

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Magnetophoresis-Enhanced Elasto-Inertial Migration of Microparticles and Cells in Microfluidics

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(9), P. 3925 - 3932

Published: Feb. 12, 2024

Microfluidic particle and cell manipulation techniques possess many potentials for biomedicine healthcare. Many have been developed based on active (e.g., electrical, magnetic, acoustic, thermal) force fields passive hydrodynamic forces inertial elastic lift forces). However, a single or manipulating physics cannot always meet the demands, combining multiple becomes promising strategy to promote technique flexibility versatility. In this work, we explored physical coupling of magnetophoresis with (i.e., elasto-inertial) microparticles. Particle lateral migration was studied in coflowing configuration viscoelastic ferrofluid/water (sample/sheath). The particles were suspended ferrofluid confined near channel sidewall by sheath flow. coordination elasto-inertial promoted cross-stream particles. Besides, investigated effect flow rate ratio total Furthermore, also effects fluid elasticity sample flows using different combinations flows, including Newtonian ferrofluid/water, ferrofluid/viscoelastic fluid, coflows. Experimental results demonstrated ascertained PEO-based coflow. Finally, demonstrate proof-of-concept application solution exchange. We envisage that novel multiphysical scheme has great potential flexible versatile microparticles cells.

Language: Английский

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Dielectrophoretic cell sorting with high velocity enabled by two-layer sidewall microelectrodes extending along the entire channel

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 410, P. 135669 - 135669

Published: March 20, 2024

Language: Английский

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Particle manipulation under X-force fields

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.

Language: Английский

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