Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 73 - 87
Published: Jan. 1, 2024
Language: Английский
Metabolites, Journal Year: 2023, Volume and Issue: 13(5), P. 625 - 625
Published: May 2, 2023
Due to the continued emergence of resistance and a lack new promising antibiotics, bacterial infection has become major public threat. High-throughput screening (HTS) allows rapid large collection molecules for bioactivity testing holds promise in antibacterial drug discovery. More than 50% antibiotics that are currently available on market derived from natural products. However, with easily discoverable being found, finding sources seen limited success. Finding activity also proven be challenging. In addition exploring products synthetic biology, omics technology helped study biosynthetic machinery existing enabling construction unnatural synthesizers bioactive identification molecular targets agents. On other hand, newer smarter strategies have been continuously pursued screen molecule libraries druggable targets. Biomimetic conditions explored mimic real model better ligand–target interaction enable designing more effective drugs. This narrative review describes various traditional contemporaneous approaches high-throughput It further discusses critical factors HTS assay design, makes general recommendation, possible alternatives
Language: Английский
Citations
49
Nature Reviews Microbiology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 17, 2025
Language: Английский
Citations
4
Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(25), P. 9782 - 9795
Published: June 13, 2023
Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease in citrus and results significant economic losses. The genus Beauveria recognized as promising source of biocontrol agents for agricultural applications. Herein, we established targeted strategy integrating genomics metabolomics to accelerate the discovery new cyclopeptides from antagonistic metabolites produced marine-derived fungus felina SYSU-MS7908. As result, isolated characterized seven cyclopeptides, including six molecules, isaridins I-N (1-6). Their chemical structures conformational analysis were extensively elucidated using spectroscopic techniques (NMR, HRMS, MS'MS data), modified Mosher's Marfey's methods, single-crystal X-ray diffraction. Notably, isaridin K (3) contains peptide backbone with an N-methyl-2-aminobutyric acid residue rarely found natural cyclopeptides. Bioassays showed that compound 2 could significantly inhibit mycelial growth G. citri-aurantii destroying cell membrane. These findings provide effective searching fungal peptides potential agrochemical fungicides also pave way further exploration applications agriculture, food, medicine.
Language: Английский
Citations
34
Nature Chemical Biology, Journal Year: 2023, Volume and Issue: 19(7), P. 846 - 854
Published: March 6, 2023
Language: Английский
Citations
30
Brain Research, Journal Year: 2024, Volume and Issue: 1843, P. 149123 - 149123
Published: Nov. 1, 2024
Language: Английский
Citations
16
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 175, P. 117711 - 117711
Published: April 21, 2024
Language: Английский
Citations
15
Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)
Published: April 17, 2025
Abstract Although cancer chemopreventive agents have been confirmed to effectively protect high-risk populations from invasion or recurrence, only over ten drugs approved by the U.S. Food and Drug Administration. Therefore, screening potent is crucial reduce constantly increasing incidence mortality rate of cancer. Considering lengthy prevention process, an ideal agent should be nontoxic, inexpensive, oral. Natural compounds become a natural treasure reservoir for chemoprevention because their superior ease availability, cost-effectiveness, safety. The benefits as in various studies. In light this, present review intended fully delineate entire scope chemoprevention, primarily focuses on aspects based compounds, specifically focusing mechanism action prevention, discussing detail how they exert effects affecting classical signaling pathways, immune checkpoints, gut microbiome. We also introduce novel strategies summarize role improving chemotherapy regimens. Furthermore, we describe discovering anticancer with low abundance high activity, revealing broad prospects drug discovery chemoprevention. Moreover, associate precision medicine, discuss challenges encountered Finally, emphasize transformative potential advancing field ability more effective less toxic preventive options oncology.
Language: Английский
Citations
1
Molecules, Journal Year: 2022, Volume and Issue: 28(1), P. 157 - 157
Published: Dec. 24, 2022
Natural products (NPs) have historically played a primary role in the discovery of small-molecule drugs. However, due to advent other methodologies and drawbacks NPs, pharmaceutical industry has largely declined interest regarding screening new drugs from NPs since 2000. There are many technical bottlenecks quickly obtaining bioactive on large scale, which made NP-based drug very time-consuming, first thorny problem faced by researchers is how dereplicate crude extracts. Remarkably, with rapid development omics, analytical instrumentation, artificial intelligence technology, 2012, an efficient approach, known as tandem mass spectrometry (MS/MS)-based molecular networking (MN) analysis, was developed avoid rediscovery compounds complex natural mixtures. Then, past decade, based classical MN (CLMN), feature-based (FBMN), ion identity (IIMN), building blocks-based network (BBMN), substructure-based (MS2LDA), bioactivity-based (BMN) methods been presented. In this paper, we review basic principles, general workflow, application examples mentioned above, further research applications these methods.
Language: Английский
Citations
33
Natural Product Reports, Journal Year: 2023, Volume and Issue: 41(3), P. 347 - 369
Published: Dec. 13, 2023
Covering: 2000 to 2023Cyanobacteria produce a variety of bioactive natural products that can pose threat humans and animals as environmental toxins, but also have potential for or inspire pharmaceutical use. As oxygenic phototrophs, cyanobacteria furthermore hold great promise sustainable biotechnology. Yet, the necessary tools exploiting their biotechnological so far been established only few model strains cyanobacteria, while large untapped biosynthetic resources are hidden in slow-growing cyanobacterial genera difficult access by genetic techniques. In recent years, several approaches developed circumvent bottlenecks product research. Here, we summarize current progress has made unlocking characterizing cryptic metabolic pathways using integrated omics techniques, orphan gene cluster activation, use original producers, heterologous expression chemo-enzymatic We mainly highlighting genomic mining concepts strategies towards high-titer production from last 10 years discuss need further research developments this field.
Language: Английский
Citations
23
TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 167, P. 117248 - 117248
Published: Aug. 21, 2023
The human microbiome and its metabolic output play an important role in shaping health disease. host-microbe crosstalk is often characterised using metabolomics metagenomics, yet a large proportion of microbial metabolites the molecular functions gut microbes remain unknown. This review summarises strategies for annotation discovery novel from microbiome, either alone or combination with presents data analysis methods combining these two types to obtain biological insights. Applications research biomarker screening, precision medicine, drug are also discussed, along perspectives, challenges limitations this field.
Language: Английский
Citations
21