Water-soluble red fluorescent protein dimers for hypoxic two-photon photodynamic therapy

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(9), P. 2413 - 2424

Published: Jan. 1, 2024

In this study, two water-soluble red fluorescent protein (RFP) dimers, FP2R' and FP2R'', were synthesized by linking phenothiazine-based RFP chromophore analogues through alkyl chains or alkoxy for hypoxic two-photon photodynamic therapy. dimers are heavy-atom-free photosensitizers in which the intersystem crossing process is boosted S N heteroatoms. terms of aqueous solubility, saturation concentration FP2R'' was 3.5 mM, emission wavelength 677 nm, singlet oxygen yield 18%, absorption coefficient (β) 2.1 × 10-11 cm W-1. Further, dimer showed excellent biocompatibility, negligible dark toxicity, could produce 1O2 O2˙- simultaneously. Under 460 nm illumination, photosensitizer high phototoxicity with an IC50 value 4.08 μM hypoxia environment, indicating that has anti-hypoxia ability. addition, demonstrated a precise localization ability to lysosomes its Pearson's colocalization 0.94, guide aggregation tumor cells effectively improve therapy (PDT) effect. particular, when exposed 800 excitation, produced zebrafish exhibited bright fluorescence imaging capability. At same time, efficacy mediated verified model, growth significantly inhibited under laser irradiation. The reasonably constructed study can be used as high-efficiency inhibit deep tissues.

Language: Английский

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Macromolecular crowding sensing during osmotic stress in plants

Trends in Biochemical Sciences, Journal Year: 2024, Volume and Issue: 49(6), P. 480 - 493

Published: March 20, 2024

Language: Английский

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Strategic Modulation of Polarity and Viscosity Sensitivity of Bimane Molecular Rotor-Based Fluorophores for Imaging α-Synuclein

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Molecular rotor-based fluorophores (RBFs) that are target-selective and sensitive to both polarity viscosity valuable for diverse biological applications. Here, we have designed next-generation RBFs based on the underexplored bimane fluorophore through either changing in aryl substitution or varying π-linkages between rotatable electron donors acceptors produce red-shifted fluorescence emissions with large Stokes shifts. exhibit a twisted intramolecular charge transfer mechanism enables control of sensitivity, as well target selectivity. These features enable their application in: (1) turn-on fluorescent detection α-synuclein (αS) fibrils, hallmark Parkinson's disease (PD), including amplified fibrils from patient samples; (2) monitoring early misfolding oligomer formation during αS aggregation; (3) selective imaging condensates formed by liquid-liquid phase separation (LLPS). In all three cases, show our probes high levels selectivity versus other aggregating proteins. properties one study interplay tau amyloid aggregation mechanisms underlying neurodegenerative disorders.

Language: Английский

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Locally activated semisynthetic fluorescent biosensors for imaging cellular biochemistry

Comptes Rendus Chimie, Journal Year: 2025, Volume and Issue: 28(G1), P. 61 - 78

Published: Jan. 20, 2025

Biosensors based on fluorescent proteins are widely used as genetically encoded indicators due to their capacity target various biological analytes (metal ions, reactive oxygen species, biomolecules, etc.) within cells with precise localization. However, complex development associated the lack of photophysical versatility constrains scope application in biosensing. Alternatively, semisynthetic biosensors that combine a small chemical indicator self-labeling protein tag benefit from molecular engineering and selectivity genetic encoding recombinant protein. The variations properties upon analyte recognition provide high sensitivity rapid response time, making them attractive alternatives for Fluorogenic only local activation by reaction an additional level selectivity, allowing wash-free imaging experiments. This minireview focuses latter class hybrid sensors provides outlook different probe design strategies combinations (mostly SNAP-tag HaloTag) construction. authors expect present new clues ideas researchers further advances this field. Les biosenseurs basés sur les protéines fluorescentes sont largement utilisés comme indicateurs génétiquement encodés en raison de leur capacité à cibler divers biologiques (ions métalliques, espèces réactives l'oxygène, biomolécules, dans cellules avec une localisation précise. Cependant, développement complexe, associé des propriétés photophysiques peu versatile, limite l'étendue imagerie. En revanche, fluorescents semi-synthétiques, qui combinent un indicateur chimique étiquette marquage protéine, bénéficient la polyvalence l'ingénierie petites molécules organiques et sélectivité l'encodage génétique recombinantes. La variation l'indicateur lors reconnaissance l'analyte offrent grande sensibilité temps réponse rapide, ce fait alternative intéressante pour bioimagerie. semi-synthétiques fluorogéniques, ne deviennent que lorsqu'ils activés localement par réaction protéine encodée, niveau supplémentaire sélectivité, permettant expériences d'imagerie sans étape lavage. Cette mini-revue se concentre cette dernière classe senseurs hybrides donne aperçu différentes stratégies conception moléculaires combinaison tels HaloTag Nous espérons apporter nouvelles pistes idées aux chercheurs futures avancées domaine.

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Genetic engineering-powered dual-mode lateral flow immunosensor for colorimetric and fluorescent detection of ochratoxin A in pepper

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 489, P. 137636 - 137636

Published: Feb. 16, 2025

Language: Английский

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