Chinese Herbal Medicines,
Journal Year:
2024,
Volume and Issue:
17(1), P. 148 - 155
Published: March 26, 2024
Myocardial
inflammation
during
myocardial
infarction
(MI)
could
be
inhibited
by
regulating
arachidonic
acid
(AA)
metabolism.
Recent
studies
demonstrated
that
Sini
Decoction
(SND)
was
identified
to
an
effective
prescription
for
treating
heart
failure
(HF)
caused
MI.
But
the
anti-inflammatory
mechanism
of
SND
remained
unclear.
The
work
designed
investigate
through
AA
metabolism
pathway
in
vitro
and
vivo
experiments.
An
inflammatory
injury
model
H9c2
cells
established
lipopolysaccharide
(LPS)-stimulated
macrophage-conditioned
media
(CM).
animal
MI
built
ligation
left
anterior
descending
(LAD)
branch
coronary
artery
rat.
Meanwhile,
rats
were
divided
into
five
groups:
sham
group,
+
Celecoxib
low-dose
group
(SND-L)
high-dose
(SND-H).
Cardiac
function,
histopathological
changes
serum
cytokines
examined
four
weeks
later.
Western
blot
analysis
conducted
verify
key
enzymes
levels
metabolic
pathway,
including
phospholipase
A2
(PLA2),
cyclooxygenases
(COXs)
lipoxygenases
(LOXs).
These
results
improve
cardiac
function
pathological
with
MI,
regulate
molecules
sPLA2,
COX-1,
COX-2,
5-LOX
15-LOX.
In
vitro,
decrease
release
pro-inflammatory
TNF-α
IL-6
inhibit
cell
apoptosis
CM-induced
cells.
Moreover,
protect
from
damage
CM
nuclear
factor
kappa-B
(NF-κB)
signal
expression
COX-2.
may
a
drug
candidate
treatment
multiple
targets
pathway.
Lipids in Health and Disease,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Feb. 11, 2025
The
impact
of
a
high-salt
(HS)
diet
on
metabolic
disturbances
in
individuals
with
coronary
heart
disease
remains
unclear.
arachidonic
acid
(AA)
pathway
is
closely
linked
to
the
development
cardiometabolic
diseases
and
atherosclerotic
cardiovascular
diseases.
Furthermore,
endoplasmic
reticulum
stress
(ERS)
has
emerged
as
major
contributor
AA-related
inflammation
ERS
are
hypothesized
play
role
HS
diet-induced
remodeling.
Rats
were
subjected
an
for
4
weeks,
serum
concentration
AA
was
measured
via
enzyme-linked
immunosorbent
assay.
Immunofluorescence
staining
vascular
tension
measurements
conducted
arteries.
In
addition,
AA-stimulated
artery
smooth
muscle
cells
(CASMCs)
treated
inhibitors
explore
underlying
involved.
Increased
susceptibility
myocardial
infarction
diet-fed
rats
accompanied
by
increased
concentrations
expression
key
enzyme
cyclooxygenase-2
(COX-2).
incubation
weakened
contraction
denuded
arteries,
reduced
markers,
fluorescence
intensity
synthetic
response
markers
Further
investigation
CASMCs
revealed
that
AA-induced
phenotypic
transformation
mediated
pathway.
found
be
stimulated
following
diet.
triggers
through
COX-2
catalysis,
downstream
inositol
requiring
1
-
X-box
binding
protein-1
osteopontin
may
contribute
CASMCs,
resulting
dysfunctional
tension.
This
study
provide
potential
therapeutic
targets
associated
excessive
AA-derived
ERS.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(17), P. 13227 - 13227
Published: Aug. 25, 2023
Pulmonary
hypertension
(PH)
is
a
multifaceted
illness
causing
clinical
manifestations
like
dyspnea,
fatigue,
and
cyanosis.
If
left
untreated,
it
often
evolves
into
irreversible
pulmonary
arterial
(PAH),
leading
to
death.
Metabolomics
laboratory
technique
capable
of
providing
insights
the
metabolic
pathways
that
are
responsible
for
number
physiologic
or
pathologic
events
through
analysis
biological
fluid
(such
as
blood,
urine,
sputum)
using
proton
nuclear
magnetic
resonance
spectroscopy
mass
spectrometry.
A
systematic
review
was
finalized
according
PRISMA
scheme,
with
goal
an
overview
research
papers
released
up
now
on
application
metabolomics
PH/PAH.
So,
eighty-five
were
identified,
which
twenty-four
concerning
PH,
sixty-one
regarding
PAH.
We
found
that,
from
standpoint,
hallmarks
disease
onset
progression
increase
in
glycolysis
impaired
mitochondrial
respiration.
Oxidation
exacerbated
well.
Specific
fingerprints
allow
characterization
some
specific
PH
PAH
subtypes.
Overall,
provides
processes
happening
body
subject
suffering
The
disarranged
underpinning
may
be
target
new
therapeutic
agents.
will
investigators
make
step
forward
towards
personalized
medicine.
Journal of Food Biochemistry,
Journal Year:
2024,
Volume and Issue:
2024(1)
Published: Jan. 1, 2024
Purpose:
Pulmonary
arterial
hypertension
(PAH)
is
a
fatal
condition
characterized
by
poor
control
of
pulmonary
hemodynamics
and
vascular
development.
Zang
Siwei
Qingfei
mixture
(ZSQM)
traditional
Chinese
medicine
formula
used
for
the
treatment
chronic
respiratory
diseases.
However,
underlying
mechanism
ZSQM
treating
PAH
remains
unclear.
Methods:
A
rat
model
was
established
after
monocrotaline
(MCT)
injection,
hemodynamic
features
pathological
changes
were
evaluated.
The
candidate
targets
against
discovered
using
network
pharmacology.
Then,
molecular
docking
to
validate
key
targets.
Moreover,
serum
metabolomics
identify
differential
metabolites.
pharmacology
integrated
constructed.
Results:
alleviated
MCT‐induced
injury
remodeling.
We
found
core
component
chrysin
six
hub
genes
according
pharmacology,
including
CYP2C19,
CASP8,
PTK2,
VEGFA,
FLT4,
TNNI3.
Molecular
revealed
strong
binding
affinities
between
chrysin.
Meanwhile,
western
blotting
results
validated
significant
in
expression
these
Subsequently,
we
confirmed
that
existed
HPLC–MS.
In
addition,
total
19
metabolites
with
potential
significance
identified
be
implicated
therapeutic
mechanisms
ZSQM.
further
analysis
indicated
an
interconnection
targets,
their
related
(12(S)HETE,
ascorbate,
succinate,
vitamin
C,
L‐arginine),
metabolic
pathways.
Conclusion:
study,
employing
has
concluded
enhance
targeting
multiple
pathways
This
finding
suggests
could
serve
as
promising
alternative
therapy
PAH.
