Sini decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism DOI Open Access

Cuiping Long,

Qian Zhou, Min Xu

et al.

Chinese Herbal Medicines, Journal Year: 2024, Volume and Issue: 17(1), P. 148 - 155

Published: March 26, 2024

Myocardial inflammation during myocardial infarction (MI) could be inhibited by regulating arachidonic acid (AA) metabolism. Recent studies demonstrated that Sini Decoction (SND) was identified to an effective prescription for treating heart failure (HF) caused MI. But the anti-inflammatory mechanism of SND remained unclear. The work designed investigate through AA metabolism pathway in vitro and vivo experiments. An inflammatory injury model H9c2 cells established lipopolysaccharide (LPS)-stimulated macrophage-conditioned media (CM). animal MI built ligation left anterior descending (LAD) branch coronary artery rat. Meanwhile, rats were divided into five groups: sham group, + Celecoxib low-dose group (SND-L) high-dose (SND-H). Cardiac function, histopathological changes serum cytokines examined four weeks later. Western blot analysis conducted verify key enzymes levels metabolic pathway, including phospholipase A2 (PLA2), cyclooxygenases (COXs) lipoxygenases (LOXs). These results improve cardiac function pathological with MI, regulate molecules sPLA2, COX-1, COX-2, 5-LOX 15-LOX. In vitro, decrease release pro-inflammatory TNF-α IL-6 inhibit cell apoptosis CM-induced cells. Moreover, protect from damage CM nuclear factor kappa-B (NF-κB) signal expression COX-2. may a drug candidate treatment multiple targets pathway.

Language: Английский

Sini decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism DOI Open Access

Cuiping Long,

Qian Zhou, Min Xu

et al.

Chinese Herbal Medicines, Journal Year: 2024, Volume and Issue: 17(1), P. 148 - 155

Published: March 26, 2024

Myocardial inflammation during myocardial infarction (MI) could be inhibited by regulating arachidonic acid (AA) metabolism. Recent studies demonstrated that Sini Decoction (SND) was identified to an effective prescription for treating heart failure (HF) caused MI. But the anti-inflammatory mechanism of SND remained unclear. The work designed investigate through AA metabolism pathway in vitro and vivo experiments. An inflammatory injury model H9c2 cells established lipopolysaccharide (LPS)-stimulated macrophage-conditioned media (CM). animal MI built ligation left anterior descending (LAD) branch coronary artery rat. Meanwhile, rats were divided into five groups: sham group, + Celecoxib low-dose group (SND-L) high-dose (SND-H). Cardiac function, histopathological changes serum cytokines examined four weeks later. Western blot analysis conducted verify key enzymes levels metabolic pathway, including phospholipase A2 (PLA2), cyclooxygenases (COXs) lipoxygenases (LOXs). These results improve cardiac function pathological with MI, regulate molecules sPLA2, COX-1, COX-2, 5-LOX 15-LOX. In vitro, decrease release pro-inflammatory TNF-α IL-6 inhibit cell apoptosis CM-induced cells. Moreover, protect from damage CM nuclear factor kappa-B (NF-κB) signal expression COX-2. may a drug candidate treatment multiple targets pathway.

Language: Английский

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