Neuropathogenesis-on-chips for neurodegenerative diseases

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 12, 2024

Abstract Developing diagnostics and treatments for neurodegenerative diseases (NDs) is challenging due to multifactorial pathogenesis that progresses gradually. Advanced in vitro systems recapitulate patient-like pathophysiology are emerging as alternatives conventional animal-based models. In this review, we explore the interconnected pathogenic features of different types ND, discuss general strategy modelling NDs using a microfluidic chip, introduce organoid-on-a-chip next advanced relevant model. Lastly, overview how these models being applied academic industrial drug development. The integration chips, stem cells, biotechnological devices promises provide valuable insights biomedical research developing diagnostic therapeutic solutions NDs.

Language: Английский

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Humanized brain organoids-on-chip integrated with sensors for screening neuronal activity and neurotoxicity

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(1)

Published: Jan. 1, 2024

Language: Английский

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Wearable Insulin Biosensors for Diabetes Management: Advances and Challenges

Biosensors, Journal Year: 2023, Volume and Issue: 13(7), P. 719 - 719

Published: July 7, 2023

We present a critical review of the current progress in wearable insulin biosensors. For over 40 years, glucose biosensors have been used for diabetes management. Measurement blood is an indirect method calculating administration dosage, which insulin-dependent diabetic patients. Research and development efforts aiming towards continuous-insulin-monitoring combination with existing are expected to offer more accurate estimation sensitivity, regulate dosage facilitate reliable artificial pancreas, as ultimate goal management personalised medicine. Conventional laboratory analytical techniques detection expensive time-consuming lack real-time monitoring capability. On other hand, point-of-care testing, continuous monitoring, miniaturisation, high specificity rapid response time, ease use low costs. Current research, future developments challenges biosensor technology reviewed assessed. Different categories such aptamer-based, molecularly imprinted polymer (MIP)-based, label-free types presented among latest field. This multidisciplinary field requires engagement between scientists, engineers, clinicians industry addressing commercial, reliable, real-time-monitoring biosensor.

Language: Английский

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Living cell‐laden hydrogels: Unleashing the future of responsive biohybrid systems

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(1)

Published: Aug. 1, 2023

Abstract Responsive biohybrid systems have the potential to overcome limitations of both natural and artificial machines in terms efficiency, accuracy, functionality. As functional units, living cells act as bricks for building machines, where extracellular matrix mimics hydrogels ideal biological concrete. Combining with offers unique advantages simulating human tissues or organs, which unleashes future systems, thus has attracted extensive attention. Herein, recent progress cell‐laden hydrogel‐based responsive is summarized provide a basic understanding how these are built from bottom up achieve complex functions. This review focuses on advanced manufacturing technologies including laden hydrogel matrices, three‐dimensional bioprinting, microfluidic manufacturing. Subsequently, innovative applications works, actuators, sensors, engineered materials, presented, along different triggering mechanisms that highlighted. Finally, current challenges opportunities field addressed. provides perspective hoped inspire fields such technologies, soft robots, tissue engineering.

Language: Английский

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Organ-on-a-chip platforms integrated with biosensors for precise monitoring of the cells and cellular microenvironment

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117569 - 117569

Published: Jan. 30, 2024

Language: Английский

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Application of Polymethylpentene, an Oxygen Permeable Thermoplastic, for Long-Term on-a-Chip Cell Culture and Organ-on-a-Chip Devices

Micromachines, Journal Year: 2023, Volume and Issue: 14(3), P. 532 - 532

Published: Feb. 24, 2023

The applicability of a gas-permeable, thermoplastic material polymethylpentene (PMP) was investigated, experimentally and analytically, for organ-on-a-chip (OoC) long-term on-a-chip cell cultivation applications. Using sealed culture chamber device fitted with oxygen sensors, we tested compared PMP to commonly used glass polydimethylsiloxane (PDMS). We show that PDMS have comparable performance supply during 4 days epithelial (A549) cells concentration stabilizing at 16%, control where it decreases 3%. For the first time, transmission light images growing on were obtained, demonstrating optical properties are suitable non-fluorescent, live imaging. Following combined imaging calcein-AM staining, adherence, proliferation, morphology, viability A549 shown be similar coated poly-L-lysine. In contrast PDMS, demonstrate film as thin 0.125 mm is compatible high-resolution confocal microscopy due its excellent mechanical stiffness. also found fully sterilization, fixation, permeabilization fluorescent staining. envision this extend range possible microfluidic applications beyond current state-of-the-art, beneficial physical suitability prototyping by different methods. integrated measurement methodology demonstrated in work transferrable other cell-based studies life-sciences

Language: Английский

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Microfluidic-based skin-on-chip systems for safety assessment of nanomaterials

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 41(10), P. 1282 - 1298

Published: July 5, 2023

The skin is the body's largest organ, continuously exposed to and affected by natural anthropogenic nanomaterials (materials with external internal dimensions in nanoscale range). This broad spectrum of insults gives rise irreversible health effects (from corrosion cancer). Organ-on-chip systems can recapitulate physiology high fidelity potentially revolutionize safety assessment nanomaterials. Here, we review current advances skin-on-chip models their potential elucidate biological mechanisms. Further, strategies are discussed on-chip, improving control over exposure transport across cells. Finally, highlight future opportunities challenges from design fabrication acceptance regulatory bodies industry.

Language: Английский

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Microfluidic organ-on-chip systems for periodontal research: advances and future directions

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 7, 2025

Advances in tissue engineering and microfluidic technologies have enabled the development of sophisticated vitro models known as organ-on-a-chip (OoC) or microphysiological systems. These systems enable to potential simulate dynamic interactions between host tissues their microenvironment including microbes, biomaterials, mechanical forces, pharmaceutical, consumer-care products. fluidic are increasingly being utilized investigate host-microbe host-material oral health disease. Of interest is application understanding periodontal disease, a chronic inflammatory condition marked by progressive destruction tissues, gingiva, ligament, alveolar bone. The pathogenesis disease involves complex interplay microbial dysbiosis immune responses, which can lead loss dental support structures contribute systemic conditions such cardiovascular diabetes, bowel This provides comprehensive overview latest developments millifluidic designed emulate interactions. We discuss critical biological considerations designing these platforms, applications studying biofilms, unravel mechanisms therapeutic targets

Language: Английский

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Recent Advances in Integrated Organ‐Chip Sensing Toward Robust and User‐Friendly Systems

Journal of Biomedical Materials Research Part A, Journal Year: 2025, Volume and Issue: 113(2)

Published: Feb. 1, 2025

Organs-on-a-chip (OOC) are an emergent technology that bridge the gap between current in vitro and vivo models used to inform drug discovery investigate disease pathophysiology. These systems offer improved bio-relevance controlled complexity through integration of physical and/or chemical stimuli matched physiologically relevant conditions. Although significant advancements have been made toward recreating organ-specific physiology on chip, methods available study structure function cell microenvironment still limited. Established analysis approaches, including fluorescence microscopy, rely laborious offline workflows yield limited time-point data. As OOC field continues evolve, there is a unique opportunity engineer characterization into organ-chip devices. This review provides overview integrated sensing approaches address limitations enable real-time readout physiological parameters OOC.

Language: Английский

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Organic Bioelectronics in Microphysiological Systems: Bridging the Gap Between Biological Systems and Electronic Technologies

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 253 - 253

Published: April 16, 2025

The growing burden of degenerative, cardiovascular, neurodegenerative, and cancerous diseases necessitates innovative approaches to improve our pathophysiological understanding ability modulate biological processes. Organic bioelectronics has emerged as a powerful tool in this pursuit, offering unique interact with biology due the mixed ionic–electronic conduction tissue-mimetic mechanical properties conducting polymers (CPs). These materials enable seamless integration systems across different levels complexity, from monolayers complex 3D models, microfluidic chips, even clinical applications. CPs can be processed into diverse formats, including thin films, hydrogels, scaffolds, electrospun fibers, allowing fabrication advanced bioelectronic devices such multi-electrode arrays, transistors (EGOFETs, OECTs), ion pumps, photoactuators. This review examines CP-based vivo vitro microphysiological systems, focusing on their monitor key events, electrical activity, metabolic changes, biomarker concentrations, well potential for electrical, mechanical, chemical stimulation. We highlight versatility biocompatibility role advancing personalized medicine regenerative therapies discuss future directions organic bridge gap between electronic technologies.

Language: Английский

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