Published: Jan. 1, 2024
Language: Английский
ACS Sensors, Journal Year: 2024, Volume and Issue: 9(6), P. 3066 - 3074
Published: June 15, 2024
Point-of-care testing (POCT) devices play a crucial role as tools for disease diagnostics, and the integration of biorecognition elements with electronic components into these widens their functionalities facilitates development complex quantitative assays. Unfortunately, biosensors that exploit large conventional IgG antibodies to capture relevant biomarkers are often limited in terms sensitivity, selectivity, storage stability, considerably restricting use POCT real-world applications. Therefore, we used nanobodies they more suitable fabricating electrochemical near-field communication (NFC) technology. Moreover, flow-through microfluidic device was implemented this system detection C-reactive protein (CRP), an inflammation biomarker, model analyte. The resulting sensors not only have high sensitivity portability but also retain automated sequential flow properties through capillary transport without need external pump. We compared accuracy CRP analyses between commercial PalmSens4 NFC-based potentiostats. Furthermore, sensor reliability evaluated using three biological samples (artificial serum, plasma, whole blood any pretreatment). This platform will streamline by combining operational simplicity, low cost, fast analysis, portability.
Language: Английский
Citations
17
Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(2)
Published: Jan. 31, 2024
Abstract Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause cervical cancer, and an important factor in other malignancies, for example, head neck cancer. Despite recent progress screening vaccination, the incidence mortality are still relatively high, especially low‐income countries. The financial burden associated treatment could be decreased if simple, rapid, inexpensive technology HPV testing becomes available, targeting individuals further monitoring increased risk developing Commercial tests available market often expensive, time‐consuming, require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies being implemented also diagnostics that include isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well microfluidics, paperfluidics lab‐on‐a‐chip devices, ideal point‐of‐care decentralized settings. In this review, we first evaluate current commercial tests, followed by description advanced technologies, explanation principles, critical evaluation strengths weaknesses, suggestions possible implementation into medical diagnostics.
Language: Английский
Citations
14
Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(9), P. 2371 - 2396
Published: Jan. 1, 2024
Over the past decade, additive manufacturing-or "three-dimensional (3D) printing"-has attracted increasing attention in
Language: Английский
Citations
9
Biomedical Engineering and Computational Biology, Journal Year: 2023, Volume and Issue: 14
Published: Jan. 1, 2023
Microfluidic systems offer versatile biomedical tools and methods to enhance human convenience health. Advances in these enables next-generation microfluidics that integrates automation, manipulation, smart readout systems, as well design three-dimensional (3D) printing for precise production of microchannels other microstructures rapidly with great flexibility. These 3D-printed microfluidic platforms not only control the complex fluid behavior various applications, but also serve microconduits building 3D tissue constructs—an integral component advanced drug development, toxicity assessment, accurate disease modeling. Furthermore, integration emerging technologies, such microscopy robotics, spatiotemporal manipulation high-throughput screening cell physiology within precisely controlled microenvironments. Notably, portability high precision automation capabilities integrated facilitate rapid experimentation data acquisition help deepen our understanding biological their behaviors. While certain challenges, including material compatibility, scaling, standardization still exist, artificial intelligence, Internet Things, materials, miniaturization holds tremendous promise reshaping traditional approaches. This transformative potential, when has potential revolutionize research healthcare ultimately benefiting review highlights advances field emphasizes critical role next generation advancing research, point-of-care diagnostics, systems.
Language: Английский
Citations
20
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 266, P. 116718 - 116718
Published: Aug. 30, 2024
Language: Английский
Citations
6
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(37), P. 14980 - 14988
Published: Sept. 5, 2024
PD-L1-positive extracellular vesicles (PD-L1
Citations
6
Biomicrofluidics, Journal Year: 2024, Volume and Issue: 18(1)
Published: Jan. 1, 2024
The robust identification and quantification of various biomarkers is utmost significance in clinical diagnostics precision medicine. Fluorescent immunoassays are widely used considered as a gold standard for biomarker detection due to their high specificity accuracy. However, current commercial immunoassay tests suffer from limited sensitivity complicated, labor-intensive operation procedures, making them impractical point-of-care diagnosis, particularly resource-limited regions. Recently, microfluidic devices integrated with plasmonic nanostructures have emerged powerful tool sensitive biomarkers, addressing specific issues, such integration schemes, easy operation, multiplexed detection, enhancement. In this paper, we provide discussion on the recent advances fluorescent immunoassays. We shed light nanofabrication strategies fluidic designs rapid, sensitive, highly efficient sensing antigens. Finally, share our perspectives potential directions these practical applications.
Language: Английский
Citations
4
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 11, 2025
The low abundance, complex phenotypes, and need for sophisticated blood preprocessing pose substantial obstacles to the clinical implementation of circulating tumor cells (CTCs). Herein, we constructed a cascaded PMMA chip-based platform separation CTCs from other within samples, as well distinguishing detection epithelial mesenchymal CTCs. primary physical chip (PS-Chip) focused sorted whole via Dean flow fractionation (DFF) according size differences between cells, being capable eliminating approximately 93.7% red (RBCs) 68.4% white (WBCs) while maintaining CTC recovery rate around 90%. Subsequently, further purify isolated in upstream, partitioned immunoaffinity capture (PICD-Chip) featuring with two independent chambers (Zone 1, Zone 2) was designed, each which premodified Gel-GO/E/V-Apt complexes that specifically recognize distinct enabling residual upstream isolation. Upon subsequent introduction probes, namely EpCAM vimentin aptamer-modified mesoporous Pt nanoparticles (mPtNPs/E/V-Apt), into 1 2, respectively, heterogeneous ranging 5 200/mL captured were distinguished quantified utilizing exceptional peroxidase activity mPtNPs. integrated approach efficient enrichment differentiation phenotypic under requirement high purity has enabled successful application diagnosis colon cancer patients at different stages.
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 187, P. 118208 - 118208
Published: Feb. 27, 2025
Language: Английский
Citations
0
Colloids and Surfaces B Biointerfaces, Journal Year: 2025, Volume and Issue: unknown, P. 114675 - 114675
Published: April 1, 2025
Language: Английский
Citations
0