Development of reduced graphene oxide (rGO) reinforced poly(lactic) acid/ cellulose nanocrystal composite through melt mixing: Effect of nanofiller on thermal, structural, biodegradation and antibacterial properties

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 204, P. 117307 - 117307

Published: Aug. 26, 2023

Language: Английский

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Effective Endotoxin Removal from Chitosan That Preserves Chemical Structure and Improves Compatibility with Immune Cells

Polymers, Journal Year: 2023, Volume and Issue: 15(7), P. 1592 - 1592

Published: March 23, 2023

Chitosan is one of the most researched biopolymers for healthcare applications, however, being a naturally derived polymer, it susceptible to endotoxin contamination, which elicits pro-inflammatory responses, skewing chitosan's performance and leading inaccurate conclusions. It therefore critical that endotoxins are quantified removed in vivo use. Here, heat mild NaOH treatment investigated as facile removal methods from chitosan. Both treatments effectively below FDA limit medical devices (<0.5 EU/mL). However, co-culture with peripheral blood mononuclear cells (PBMCs), only NaOH-treated chitosan prevented TNF-α production. While principal task, preservation structure vital synthesis lysozyme degradation chitosan-based hydrogels. The chemical properties (by FTIR-ATR) were significantly similar its native composition, whereas heat-treated evidenced macroscopic physical changes associated Maillard reaction, deeming this unsuitable further applications. Degradation studies conducted demonstrated rates chitosan-genipin hydrogels similar. In vitro showed did not negatively affect cell viability monocyte-derived dendritic (moDCs), nor induce phenotypical maturation or cytokine release.

Language: Английский

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MnO₂ Decorated Metal‐Organic Framework‐Based Hydrogel Relieving Tumor Hypoxia for Enhanced Photodynamic Therapy

Macromolecular Rapid Communications, Journal Year: 2023, Volume and Issue: 44(19)

Published: July 5, 2023

Photodynamic therapy (PDT) has emerged as a promising cancer treatment modality; however, its therapeutic efficacy is greatly limited by tumor hypoxia. In this study, metal-organic framework (MOF)-based hydrogel (MOF Gel) system that synergistically combines PDT with the supply of oxygen designed. Porphyrin-based Zr-MOF nanoparticles are synthesized photosensitizer. MnO2 decorated onto surface MOF, which can effectively convert H₂O₂ into oxygen. Simultaneously, incorporation -decorated MOF (MnP NPs) chitosan serves to enhance stability and retention at site. The results show integrated approach significantly improves inhibition efficiency relieving hypoxia enhancing PDT. Overall, findings underscore potential for employing nano-MOF-based systems agents therapy, thus advancing application multifunctional MOFs in treatment.

Language: Английский

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Uncovering the Dominant Role of Ionic Strength in Chitosan-Genipin and Chitosan-Genipin-Apatite Wollastonite Gelation Kinetics and Resulting Hydrogel Properties

Published: Jan. 1, 2025

Language: Английский

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Entrapment of Geobacillus kaustophilus lipase in ZIF-8 and cross-linking with genipin for biodiesel production from vegetable oils

Molecular Catalysis, Journal Year: 2025, Volume and Issue: 576, P. 114919 - 114919

Published: Feb. 19, 2025

Language: Английский

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Self‐Healable Hydrogel for Regression of Liver Fibrosis

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Liver fibrosis is considered as a wound healing process in the presence of chronic hepatic injury. A hydrogel (CPDP) based on chitosan-phenol that undergoes fast gelling and owns self-healing injectable properties was investigated for effect regression liver fibrosis. For purpose, we established both vitro vivo models implanted CPDP into injured liver. The not only provided suitable microenvironment hepatocyte spheroids, but also demonstrated potential spheroid-embedded system to mimic tissue vitro. Furthermore, urea synthesis hepatocytes cultured 1.12 times higher than collagen after 7 days culture, indicating effectively rescued function hepatocytes. Moreover, injury alleviated with improved model vivo. reduction approximately 28% serum AST/ALT ratios 70% decrease fibrotic area suggested 2 weeks administration. These findings suggest holds promise applications engineering.

Language: Английский

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Chitosan, derivatives, and its nanoparticles: Preparation, physicochemical properties, biological activities, and biomedical applications – A comprehensive review

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142832 - 142832

Published: April 1, 2025

Language: Английский

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Co-Encapsulation of Phycocyanin and Albumin-Bound Curcumin in Biopolymeric Hydrogels

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3805 - 3805

Published: April 17, 2025

Co-encapsulation of hydrophilic and hydrophobic compounds within a single delivery system remains significant challenge across various scientific industrial fields. Towards this direction, an encapsulation strategy is proposed, enabling the simultaneous incorporation both biomolecules hydrogel matrix. Specifically, cyanobacterial protein phycocyanin (hydrophilic), extracted purified by dry Arthrospira maxima biomass, curcumin (hydrophobic) bound to bovine serum albumin (BSA) were utilized. This approach facilitates indirect entrapment molecules network. The structural physicochemical properties resulting hydrogels characterized using optical analysis, scanning electron microscopy (SEM), confocal laser (CLSM). Additionally, antioxidant potential encapsulated was evaluated assess their functionality after encapsulation. Furthermore, cell viability assay confirmed hydrogel’s biocompatibility lack toxicity, demonstrating its suitability as multifunctional biomaterial for biomedical pharmaceutical applications.

Language: Английский

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A new vision upon hemodialysis: a shift from synthetic to sustainable chitosan membranes

Biomedical Engineering Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100175 - 100175

Published: May 1, 2025

Language: Английский

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Blue-Light Photoactivated Curcumin-Loaded Chitosan Nanoparticles Prepared by Nanoprecipitation and Ionic Gelation: A Promising Approach for Antimicrobial Photodynamic Inactivation

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Antimicrobial photodynamic inactivation (aPDI) represents a promising alternative strategy for combating bacterial infections. This study investigates the potential of curcumin-loaded chitosan nanoparticles (CurChNPs) as novel nanoenabled photosensitizer agents photoinactivation. CurChNPs were synthesized using an innovative dual synthesis approach by combination nanoprecipitation and ionic gelation methods; their physicochemical properties also characterized. The exhibited excellent solubility in aqueous solutions, high curcumin encapsulation efficiency (96%), controlled release profile. Photoinactivation assays conducted against Staphylococcus aureus (ATCC 25923) Escherichia coli 25922) to evaluate efficacy aPDI. significant photobactericidal activity when irradiated with blue light (450 nm, 28.84 mW·cm-2). Mechanistic studies confirmed generation reactive oxygen species (ROS) primary mode Microscopy analyses revealed structural damage cell membranes, culminating lysis. These findings highlight synergistic effects antimicrobial chitosan, demonstrating that are platform eradication work contributes development sustainable, nanotechnology-based approaches addressing infections, particularly resilient Gram-negative pathogens. Future may explore antibiotic-resistant strains.

Language: Английский

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