Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 18, 2023
Abstract
Cancer
stem
cells
play
crucial
roles
in
the
development
of
cancer
chemoresistance.
L-Theanine,
a
nonproteinogenic
amino
acid
derived
from
green
tea,
is
gaining
more
and
attentions
reversing
drug
resistance.
However,
its
lung
chemoresistance
still
unknown.
To
investigate
effects
L-Theanine
on
explore
underlying
mechanism
cancer,
we
performed
panel
experiments
vitro
combined
with
RNA-seq
analysis
demonstrated
improved
to
cisplatin
(cis-diamminedichloroplatinum;
DDP)
inhibited
stemness
DDP-resistant
but
not
non-resistant
STAT3/NOTCH1
signaling
was
potential
dominant
process
involved
improving
cancer.
Mechanistically,
impedes
migration
activation
via
regulating
expression
STAT3/NOTCH1/BMAL1
signaling-induced
markers,
reducing
proliferation
as
well
inhibiting
resistance-related
genes
cells.
In
addition,
combination
Stat3
blockade
synergistically
summary,
chemoresistant
through
signaling,
finally
The
finding
might
provide
research
evidence
for
therapeutic
options
Molecules,
Journal Year:
2023,
Volume and Issue:
28(8), P. 3407 - 3407
Published: April 12, 2023
One
of
the
main
problems
in
chemotherapy
using
platinum
drugs
as
anticancer
agents
is
resistance
phenomenon.
Synthesizing
and
evaluating
valid
alternative
compounds
challenging.
This
review
focuses
on
last
two
years
progress
studies
(II)-
(IV)-based
complexes.
In
particular,
research
reported
herein
focus
capability
some
platinum-based
to
bypass
chemotherapy,
which
typical
well-known
such
cisplatin.
Regarding
(II)
complexes,
this
deals
with
complexes
trans
conformation;
containing
bioactive
ligands,
well
those
that
are
differently
charged,
all
experience
a
different
reaction
mechanism
compared
(IV)
compounds,
was
biologically
active
ancillary
ligands
exert
synergistic
effect
(II)-active
upon
reduction,
or
for
controllable
activation
can
be
realized
thanks
intracellular
stimuli.
Cell Death Discovery,
Journal Year:
2023,
Volume and Issue:
9(1)
Published: Sept. 28, 2023
Ferroptosis
is
a
type
of
programmed
cell
death
mediated
by
iron-dependent
lipid
peroxidation
that
leads
to
excessive
in
different
cells.
distinct
from
other
forms
and
associated
with
various
diseases.
Iron
essential
for
spermatogenesis
male
reproductive
function.
Therefore,
it
not
surprising
new
evidence
supports
the
role
ferroptosis
testicular
injury.
Although
molecular
mechanism
which
induces
disease
unknown,
several
genes
pathways
have
been
linked
dysfunction.
In
this
review,
we
discuss
iron
metabolism,
ferroptosis,
related
regulatory
pathways.
addition,
analyze
endogenous
exogenous
factors
terms
metabolism
dysfunction,
as
well
summarize
relationship
between
Finally,
potential
strategies
target
treating
diseases
provide
directions
preventing
Frontiers in Pharmacology,
Journal Year:
2025,
Volume and Issue:
16
Published: March 27, 2025
Scutellarin
(SC),
a
natural
flavonoid,
has
been
expansively
employed
in
treating
innumerable
inflammation-related
diseases
due
to
its
antitumor,
antiinflammatory,
anticancer,
and
antioxidant
potential.
can
inhibit
significant
inflammatory
cell
signaling
pathways,
comprisingPI3K/Akt,
NF-κB,
MAPK,
while
activating
antioxidant-related
pathways
such
as
Nrf2
ARE.
Numerous
reviews
have
outlined
scutellarin’s
pharmacological
effects
associated
mechanisms
diseases.
Several
studies
elucidated
the
of
anticancer
activity
by
inhibiting
various
pathways;
however,
our
knowledge,
none
distinguished
potential
scutellarin
based
on
different
human
cancer
types.
Our
review
detailed
insights
about
type
body.
Furthermore,
we
also
formulations,
combinatorial
therapies,
comprehensive
mechanistic
research
deliver
enhanced
effective
treatment
options
for
patients.
This
study
will
provide
thorough
into
scutellarin,
supporting
development
promising
candidate
treatment.
Journal of Proteome Research,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 29, 2025
Cisplatin-based
chemotherapy
is
a
cornerstone
in
treating
cervical
cancer,
yet
the
efficacy
frequently
limited
by
rapid
onset
of
drug
resistance,
major
challenge
clinical
management.
To
investigate
this,
we
employed
HPV16+
human
cervix
squamous
carcinoma
cells,
SiHa
(CIS/S),
and
their
cisplatin-resistant
subline
(CIS/R)
as
model.
Using
DIA-based
proteomics,
identified
5152
protein
groups
over
50,000
peptides
with
global
FDR
<1%.
Comparative
analysis
revealed
123
differentially
expressed
proteins.
Gene
Set
Enrichment
Analysis
(GSEA)
highlighted
proteins
involved
DNA
damage,
metabolism,
repair
pathways
(RFC4,
RFC3,
RFC2,
DUT,
DDX54,
CDCA8,
CDK7,
CHAF1B,
GTF2F1),
suggesting
role
developing
acquired
cisplatin
resistance.
Pathways
related
to
mitotic
spindle
assembly
P53
signaling
were
found
be
perturbed
resistant
cells.
Next,
screened
library
approx.
240
FDA-approved
drugs
against
three
targets
four
small-molecular
ligands
potential
hits
for
further
vitro
validation.
Cabozantinib
sorafenib
gave
us
positive
results
terms
increasing
sensitivity
CIS/R
In
conclusion,
our
findings
provide
insights
into
molecular
mechanisms
underpinning
resistance
cancer
propose
novel
strategies
combating
this
through
targeted
therapies
repurposing.
Frontiers in Bioscience-Landmark,
Journal Year:
2024,
Volume and Issue:
29(6), P. 226 - 226
Published: June 21, 2024
Background:
L-Theanine,
a
nonproteinogenic
amino
acid
derived
from
green
tea,
is
being
recognized
as
an
anti-cancer
candidate.
However,
it's
roles
in
the
development
of
cancer
chemoresistance
still
unknown
and
molecular
mechanism
urgently
to
be
explored.
Methods:
The
effects
L-Theanine
on
lung
were
validated
by
Cell
Counting
Kit-8
(CCK-8)
assay,
transwell
vitro
tumor
spheroid
formation
assay;
expression
proteins
was
detected
using
polymerase
chain
reaction
(PCR)
western
blotting.
RNA-sequencing
(RNA-seq)
bioinformatics
analysis
used
identify
differentially
expressed
genes
induced
L-Theanine.
BMAL1
knockdown
overexpression
constructed
lentivirus-mediated
transfection
system.
Results:
improved
cis-diamminedichloroplatinum
(DDP)
inhibited
stemness
DDP-resistant
cells
but
not
non-resistant
cells.
results
RNA-seq
showed
that
STAT3/NOTCH1
pathway
potential
dominant
signaling
involved
improving
cancer.
Mechanistically,
impeded
migration
activation
via
regulating
STAT3/NOTCH1/BMAL1
signaling-induced
markers
well
inhibiting
levels
drug
resistance-related
genes.
In
addition,
combination
Stat3
blockade
synergistically
Conclusion:
improves
signaling,
reducing
stemness,
finding
might
provide
some
evidence
for
therapeutic
options
overcoming
cancers,
including
