Emerging mechanistic insights into the regulation of specialized metabolism in plants

Nature Plants, Journal Year: 2022, Volume and Issue: 9(1), P. 22 - 30

Published: Dec. 23, 2022

Language: Английский

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A chromosome-level genome assembly reveals that a bipartite gene cluster formed via an inverted duplication controls monoterpenoid biosynthesis in Schizonepeta tenuifolia

Molecular Plant, Journal Year: 2023, Volume and Issue: 16(3), P. 533 - 548

Published: Jan. 6, 2023

Biosynthetic gene clusters (BGCs) are regions of a genome where genes involved in biosynthetic pathway proximity. The origin and evolution plant BGCs as well their role specialized metabolism remain largely unclear. In this study, we have assembled chromosome-scale Japanese catnip (Schizonepeta tenuifolia) discovered BGC that contains multiple copies four adjacent steps the biosynthesis p-menthane monoterpenoids. This has an unprecedented bipartite structure, with mirrored separated by 260 kilobases. includes identical encoding old yellow enzyme, type flavin-dependent reductase. vitro assays virus-induced silencing revealed encodes missing isopiperitenone enzyme evolved from completely different family to reductase closely related Mentha spp., indicating convergent step. Phylogenomic analysis emerged uniquely S. tenuifolia lineage through insertion into region rich monoterpene synthases. cluster gained its structure via inverted duplication. for similarities recently described duplicated pairs longifolia genome, providing example order. work expands our understanding respect both form evolution, highlights power discovery pathways.

Language: Английский

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Characterization of the horse chestnut genome reveals the evolution of aescin and aesculin biosynthesis

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 13, 2023

Horse chestnut (Aesculus chinensis) is an important medicinal tree that contains various bioactive compounds, such as aescin, barrigenol-type triterpenoid saponins (BAT), and aesculin, a glycosylated coumarin. Herein, we report 470.02 Mb genome assembly characterize Aesculus-specific whole-genome duplication event, which leads to the formation of two biosynthesis-related gene clusters (BGCs). We also show AcOCS6, AcCYP716A278, AcCYP716A275, AcCSL1 genes within these BGCs along with seed-specific expressed AcBAHD6 are responsible for aescin. Furthermore, identify seven Aesculus-originated coumarin glycoside biosynthetic achieve de novo synthesis aesculin in E. coli. Collinearity analysis shows collinear BGC segments can be traced back early-diverging angiosperms, essential gene-encoding enzymes necessary BAT biosynthesis recruited before splitting Aesculus, Acer, Xanthoceras. These findings provide insight on evolution associated metabolites.

Language: Английский

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Triumphs and Challenges of Natural Product Discovery in the Postgenomic Era

Annual Review of Biochemistry, Journal Year: 2024, Volume and Issue: 93(1), P. 411 - 445

Published: Aug. 2, 2024

Natural products have played significant roles as medicine and food throughout human history. Here, we first provide a brief historical overview of natural products, their classification biosynthetic origins, the microbiological genetic methods used for discovery. We also describe discuss technologies that revolutionized field, which transitioned from classic genetics to genome-centric discovery approximately two decades ago. then highlight most recent advancements approaches in current postgenomic era, genome mining is standard operation high-throughput analytical allow parallel genes molecules at an unprecedented pace. Finally, new challenges faced by field future systematic heterologous expression strain-independent discovery, promises deliver more vials than ever before.

Language: Английский

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Plant (di)terpenoid evolution: from pigments to hormones and beyond

Natural Product Reports, Journal Year: 2022, Volume and Issue: 40(2), P. 452 - 469

Published: Dec. 6, 2022

Covering: up to 2014-2022.Diterpenoid biosynthesis in plants builds on the necessary production of (E,E,E)-geranylgeranyl diphosphate (GGPP) for photosynthetic pigment production, with diterpenoid arising very early land plant evolution, enabling stockpiling extensive arsenal (di)terpenoid natural products currently observed this kingdom. This review will build upon that previously published Annual Review Plant Biology, a stronger focus enzyme structure-function relationships, as well additional insights into evolution metabolism since generated.

Language: Английский

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Medicinal terpenoid UDP-glycosyltransferases in plants: recent advances and research strategies

Journal of Experimental Botany, Journal Year: 2022, Volume and Issue: 74(5), P. 1343 - 1357

Published: Dec. 27, 2022

Abstract Terpenoid glycosides have significant curative effects on many kinds of diseases. Most these compounds are derived from medicinal plants. Glycosylation is a key step in the biosynthesis terpenoids. In plants, UDP-dependent glycosyltransferases comprise large family enzymes that catalyze transfer sugars donor to acceptor form various bioactive glycosides. recent years, numerous terpenoid UDP-glycosyltransferases (UGTs) been cloned and characterized We review typical characteristics evolution terpenoid-related UGTs plants summarize advances research strategies over past 20 years. provide reference for study glycosylation skeletons biosynthetic pathways terpenoids

Language: Английский

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Whole-genome sequencing in medicinal plants: current progress and prospect

Science China Life Sciences, Journal Year: 2023, Volume and Issue: 67(2), P. 258 - 273

Published: Oct. 12, 2023

Language: Английский

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Genetic basis and selection of glyceollin elicitation in wild soybean

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 28, 2024

Glyceollins, a family of phytoalexins elicited in legume species, play crucial roles environmental stress response (e.g., defending against pathogens) and human health. However, little is known about the genetic basis glyceollin elicitation. In present study, we employed metabolite-based genome-wide association (mGWA) approach to identify candidate genes involved elicitation genetically diverse understudied wild soybeans subjected soybean cyst nematode. total, eight SNPs on chromosomes 3, 9, 13, 15, 20 showed significant associations with Six fell into two gene clusters that encode glycosyltransferases phenylpropanoid pathway were physically close one (ss715603454) chromosome 9. Additionally, transcription factors (TFs) such as MYB WRKY also found promising within linkage Notably, four 9 show epistasis strong signal for selection. The findings describe foundation biosynthesis soybeans; identified are predicted role regulation soybeans. how epistatic interactions selection influence variation natural populations deserves further investigation elucidate molecular mechanism biosynthesis.

Language: Английский

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Understanding metabolic diversification in plants: branchpoints in the evolution of specialized metabolism

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1914)

Published: Sept. 29, 2024

Plants are chemical engineers par excellence. Collectively they make a vast array of structurally diverse specialized metabolites. The raw materials for building new pathways (genes encoding biosynthetic enzymes) commonly recruited directly or indirectly from primary metabolism. Little is known about how metabolic and networks evolve in plants, what key nodes contribute to branches that lead the biosynthesis chemicals. Here we review molecular mechanisms underlying generation branchpoints. We also consider examples which metabolites formed through joining precursor molecules arising different routes, scenario greatly increases both diversity complexity Given emerging importance gene clustering helping identify enzymes pathways, further cover significance clusters relation dedicated pathways. In conclusion, an improved understanding branchpoints between will be order able predict illustrate complex structure better understand plasticity plant This article part theme issue ‘The evolution metabolism’.

Language: Английский

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Integrating cotyledon-based virus-induced gene silencing with visual marker promises a rapid, highly effective validation of gene functions in Nepeta cataria

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 21, 2025

Nepeta spp. generate volatile nepetalactone iridoids that have cat-attractant and insect-repellent activities. They differ from typical mint family (Lamiaceae) iridoids, which are non-volatile glucosides, also vary other species in the Nepetoideae sub-family, do not iridoids. The chemistry evolution of make it suitable for further investigation. However, lack transgenic technology hampers molecular genetic investigations . Virus-induced gene silencing (VIGS) is a powerful tool to detect functions vivo Here, we constructed modified VIGS method cataria , using cotyledon infiltration, with effect spreading first two pairs true leaves. efficiency reached as high 84.4%, procedure takes only 3 weeks. We employed this validate role geraniol 8-hydroxylase biosynthesis ChlH visual marker N. applicable mussinii Thus, developed an easy effective approach, will be advantageous endogenous studies holds potential application plants.

Language: Английский

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