Universal All-In-One Lateral Flow Immunoassay with Triple Signal Amplification for Ultrasensitive and Simple Self-Testing of Treponema pallidum Antibodies

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Lateral flow immunoassay (LFIA) is valued for its simplicity and rapidity on-site screening, however, it experienced false negatives in real sample analysis due to low sensitivity. Although many signal amplification techniques can improve the sensitivity, they usually require additional complicated steps. To address these issues, taking

Language: Английский

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Mechanistic Insights into Targeting SARS-CoV-2 Papain-like Protease in the Evolution and Management of COVID-19

BioChem, Journal Year: 2024, Volume and Issue: 4(3), P. 268 - 299

Published: Sept. 23, 2024

The COVID-19 pandemic, instigated by the emergence of novel coronavirus, SARS-CoV-2, created an incomparable global health crisis. Due to its highly virulent nature, identifying potential therapeutic agents against this lethal virus is crucial. PLpro a key protein involved in viral polyprotein processing and immune system evasion, making it prime target for development antiviral drugs combat COVID-19. To expedite search candidates, review delved into computational studies. Recent investigations have harnessed methods identify promising inhibitors targeting PLpro, aiming suppress activity. Molecular docking techniques were employed researchers explore binding sites within catalytic region PLpro. elucidates functional structural properties SARS-CoV-2 underscoring significance pathogenicity replication. Through comprehensive all-atom molecular dynamics (MD) simulations, stability drug–PLpro complexes was assessed, providing dynamic insights their interactions. By evaluating energy estimates from MD stable with identified. This offers overview drug/lead candidates discovered thus far using diverse silico methodologies, encompassing drug repurposing, structure-based, ligand-based virtual screenings. Additionally, identified are listed based on chemical structures meticulously examined according various parameters, such as estimated free (ΔG), types intermolecular interactions, PLpro–ligand complexes, determined outcomes simulations. Underscoring pivotal role battle COVID-19, establishes robust foundation integrating modeling, insights. continual imperative improvement existing exploring compounds remains paramount efforts evolution management hinge symbiotic relationship between experimental validation, interdisciplinary synergy crucial endeavor.

Language: Английский

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Mirror-Image RNA: A Right-Handed Z-Form RNA and Its Ligand Complex

Molecules, Journal Year: 2024, Volume and Issue: 29(20), P. 4900 - 4900

Published: Oct. 16, 2024

Until now, Z-form RNAs were believed to only adopt a left-handed double-helix structure. In this study, we describe the first observation of right-handed RNA in NMR solution formed by L-nucleic acid and present resolution structure complex between curaxin ligand. These results provide platform for design topology specific Z-form-targeting compounds are valuable development new potent anticancer drugs.

Language: Английский

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Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 18, 2024

The COVID-19 has put emphasis on pivotal needs for diagnosis and surveillance worldwide, with the subsequent shortage of diagnostic reagents kits. Therefore, it become strategic countries to access diagnostics, expand testing capacity, develop their own capabilities alternative rapid accurate nucleic acid diagnostics that are at lower costs. Here, we propose a visual SARS-CoV-2 detection using one-step fast multiplex reverse transcription-PCR (RT-PCR) amplification coupled lateral flow immunoassay PCRD device (Abingdon Health, UK). We developed various simplex fast-PCRs screening sets primer pairs newly designed or selected from literature validated WHO targeting S, N, E, RdRp ORF1ab genes. retained primers showing specific stable assess suitability PCRD. Thus, RT-PCR amplifications were performed primers. They doubly labeled Fam Biotin Dig allow amplicons PCR Detection (PCRD) device, looking lack interaction (or dimers) test-lines in negative no RNA controls. set up all assays RNAs isolated patients' nasopharyngeal swabs. used two assays, different viral genomic regions (N E) PCRD, assay (where both differently engaged) amplicons' device. evaluated this novel 50 positive samples compared its performance results quantitative (RT-qPCR) diagnosing patients, here considered as standard tests. new achieved sensitivity 88% (44/50) specificity 98% (49/50). All patients who presented Ct values than 33 our assay. Except one patient, those above returned results. Our have brought proof principle usefulness RT- specific, sensitive, without requiring costly laboratory equipment, thus, reduced costs format prone be deployed when resources limited. This offers viable points need.

Language: Английский

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Vertical Flow Immunoassay Based on Carbon Black Nanoparticles for the Detection of IgG against SARS-CoV-2 Spike Protein in Human Serum: Proof-of-Concept

Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 857 - 857

Published: Aug. 29, 2023

Point-of-care tests play an important role in serological diagnostics of infectious diseases and post-vaccination immunity monitoring, including COVID-19. Currently, lateral flow dominate this area show good analytical performance. However, studies to improve the effectiveness such remain important. In comparison with tests, vertical immunoassays allow for a reduction assay duration influence hook effect. Additionally, use carbon black nanoparticles (CNPs) as color label can provide lower detection limit (LOD) compared conventional colloidal gold. Therefore, we have developed immunoassay IgG against SARS-CoV-2 spike protein human serum samples by applying conjugate CNPs anti-human mouse monoclonal antibodies (CNP@MAb). The device consists plastic cassette hole on its top containing nitrocellulose membrane coated absorbent pad. sample, washing buffer, CNP@MAb vertically through pads, reducing time simplifying procedure. positive samples, interaction anti-spike leads appearance spots, which be visually detected. method allows rapid visual (5–7 min) vs. protein, LOD 7.81 BAU/mL. It has been shown that untrained operator perform evaluate results. Thus, presented used further development test systems COVID-19 or monitoring.

Language: Английский

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