Recent progress in biopolymer-based electrospun nanofibers and their potential biomedical applications: A review

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 293, P. 139426 - 139426

Published: Jan. 1, 2025

Language: Английский

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3D Printed Hierarchical Porous Poly(ε-caprolactone) Scaffolds from Pickering High Internal Phase Emulsion Templating

Langmuir, Journal Year: 2023, Volume and Issue: 39(5), P. 1927 - 1946

Published: Jan. 26, 2023

In the realm of biomaterials, particularly bone tissue engineering, there has been a great increase in interest scaffolds with hierarchical porosity and customizable multifunctionality. Recently, three-dimensional (3D) printing biopolymer-based inks (solutions or emulsions) gained high popularity for fabricating engineering scaffolds, which optimally satisfies desired properties performances. Herein, therefore, we explore fabrication 3D printed porous poly(ε-caprolactone) (PCL) using water-in-oil (w/o) Pickering PCL internal phase emulsions (HIPEs) as ink printer. The HIPEs stabilized hydrophobically modified nanoclay comprised aqueous poly(vinyl alcohol) (PVA) dispersed phase. Rheological measurements suggested shear thinning behavior having droplet diameter 3–25 μm. pore morphology resembling natural extracellular matrix mechanical were customized by tuning emulsion composition parameters. vitro biomineralization drug release studies proved scaffolds' potential developing apatite-rich bioactive interphase controlled delivery, respectively. During osteoblast (MG63) growth experiments up to 7 days, good adhesion proliferation on confirmed their cytocompatibility, assessed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) analysis. This study suggests that assembly HIPE templates is promising approach creating potentially suitable can be stretched other biopolymers well.

Language: Английский

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A critical review on developments in drying technologies for enhanced stability and bioavailability of pharmaceuticals

Drying Technology, Journal Year: 2024, Volume and Issue: 42(9), P. 1415 - 1441

Published: June 2, 2024

Language: Английский

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The Future of Bone Repair: Emerging Technologies and Biomaterials in Bone Regeneration

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12766 - 12766

Published: Nov. 27, 2024

Bone defects and fractures present significant clinical challenges, particularly in orthopedic maxillofacial applications. While minor bone may be capable of healing naturally, those a critical size necessitate intervention through the use implants or grafts. The utilization traditional methodologies, encompassing autografts allografts, is constrained by several factors. These include potential for donor site morbidity, restricted availability suitable donors, possibility immune rejection. This has prompted extensive research field tissue engineering to develop advanced synthetic bio-derived materials that can support regeneration. optimal substitute must achieve balance between biocompatibility, bioresorbability, osteoconductivity, osteoinductivity while simultaneously providing mechanical during process. Recent innovations three-dimensional printing, nanotechnology, bioactive coatings create scaffolds mimic structure natural enhance cell proliferation differentiation. Notwithstanding advancements above, challenges remain optimizing controlled release growth factors adapting various contexts. review provides comprehensive overview current materials, focusing on their biological mechanisms, design considerations, It explores role emerging technologies, such as additive manufacturing stem cell-based therapies, advancing field. Future highlights need multidisciplinary collaboration rigorous testing graft substitutes, improving outcomes quality life patients with complex defects.

Language: Английский

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Synergistic Functions of the Janus Fibrous Membrane for Enhanced Bone Repair

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

The treatment of bone defects presents significant challenges in clinical practice. Guided regeneration (GBR) strategies offer a new approach, but existing commercial GBR membranes still lack optimal barrier and osteogenic functions. This study the design Janus fibrous membrane using classic amphiphilic block copolymers gelatin methacryloyl containing unsaturated double bonds through gradient electrospinning process. Specifically, by controlling parameters, self-assembly copolymers, secondary photo-cross-linking, differential composition, topological structure, properties between different layers were achieved, thereby realizing synergistic physiological repair-promoting By leveraging effect, it effectively blocks adverse effects soft tissue cell ingrowth on repair while guiding proliferation differentiation. Furthermore, membrane's functionality is optimized incorporating antimicrobial component ε-poly-l-lysine niobium. In vivo studies demonstrate excellent biocompatibility, antibacterial activity, remarkable potential both normal infectious defect animal models. developed serves as versatile platform for biomedical applications, offering vast to address limitations current treatment.

Language: Английский

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A Functional 3D Biomimetic Osteon Accelerates Bone Regeneration

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Abstract The distinctive structure and composition of the Haversian canal within osteons play a pivotal role in sustaining nutritional supply to individual bone cells. Consequently, osteon's inherent advantages facilitating repair have garnered increasing attention. However, most existing designs emulate only partial aspects architecture, failing replicate its functionality comprehensively. This study aimed develop functional 3D biomimetic osteon enhance regeneration. To achieve this, oriented nanofibers mimicking osteonal lamellae are fabricated via electrospinning, while blood vessel, simulating canal, is constructed using microfluidics technology. vessels, seeded with rat umbilical vein endothelial cells, secreted morphogenetic protein stimulate osteogenesis released platelet‐derived growth factor promote angiogenesis, further supporting processes. nanofibers, composed type I collagen nano‐hydroxyapatite, calcium ions, can facilitate recruitment marrow mesenchymal stem their adhesion fibers, osteogenic differentiation. These findings demonstrate that replicating both natural function tissue provides superior strategy for repair.

Language: Английский

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Fiber diameters and parallel patterns: proliferation and osteogenesis of stem cells

Regenerative Biomaterials, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 1, 2023

Due to the innate extracellular matrix mimicking features, fibrous materials exhibited great application potential in biomedicine. In developing excellent biomaterial, it is essential reveal corresponding inherent fiber features' effects on cell behaviors. inevitable 'interference' adhesions background or between adjacent fibers, difficult precisely diameter effect behaviors by using a traditional mat. A single-layer and parallel-arranged polycaprolactone pattern platform with an non-fouling designed constructed herein. this unique material platform, through interspace fibers environment could be effectively ruled out background. The also excluded from sparsely arranged (SA) patterns. influence of stem comprehensively investigated based eliminating undesired controllable way. On SA patterns, small (SA-D1, D1 means 1 μm diameter) may seriously restrict proliferation osteogenesis when compared middle (SA-D8) large (SA-D56) ones SA-D8 shows optimal enhancement effect. At same time, cells present similar ability even highest osteogenic densely (DA) patterns (DA-D1) (DA-D8) (DA-D56) ones. adhesion under dense arrangement main reason for inducing these different behavior trends along diameters. Related results comparisons have illustrated more objectively, thus providing valuable reference guidance effective biomaterials.

Language: Английский

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A nanoparticle reinforced microporous methacrylated silk fibroin hydrogel to promote bone regeneration

Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(8), P. 2121 - 2135

Published: Jan. 1, 2024

A photo-crosslinked nanocomposite hydrogel was prepared by incorporating an osteo-inductive inorganic nanoparticle for endogenous bone regeneration.

Language: Английский

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Additive manufacturing of barium-doped calcium silicate/poly-ε-caprolactone scaffolds to activate CaSR and AKT signalling and osteogenic differentiation of mesenchymal stem cells

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(21), P. 4666 - 4676

Published: Jan. 1, 2023

3D-printed scaffolds are suitable for patient-specific implant preparation bone regeneration in large-scale critical defects. In addition, these should have mechanical and biological properties similar to those of natural tissue. this study, barium-doped calcium silicate (BaCS)/poly-ε-caprolactone (PCL) composite were fabricated as an alternative strategy tissue engineering achieve appropriate physicochemical characteristics stimulate osteogenesis. Scaffolds containing 10% Ba (Ba10) showed optimal properties, preventing premature scaffold degradation during immersion while enabling ion release a sustained manner the desired therapeutic goals. Wharton's jelly mesenchymal stem cells (WJMSCs) used assess biocompatibility osteogenic differentiation behaviour. WJMSCs cultured on permeabilised via ICP analyse presence Si ions medium cell lysates, suggesting that released by could effectively enter cells. The protein expression CaSR, PI3K, Akt, JNK confirmed CaSR activate Ba10, thereby affecting subsequent PI3k/Akt pathways further promoting differentiation. vivo performance proposed was assessed using micro-CT histological slices, which revealed BaCS enhance regeneration, compared with bare scaffolds. These results suggest potential use BaCS/PCL next-generation substitutes regeneration.

Language: Английский

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A sponge-like nanofiber melatonin-loaded scaffold accelerates vascularized bone regeneration via improving mitochondrial energy metabolism

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101078 - 101078

Published: May 3, 2024

Electrospun nanofibers have been widely employed in bone tissue engineering for their ability to mimic the micro nanometer scale network of native extracellular matrix. However, dense fibrous structure and limited mechanical support these pose challenges treatment critical size defects. In this study, we propose a facile approach creating three-dimensional scaffold using interconnected electrospun containing melatonin (Scaffold@MT). The hypothesis posited that sponge-like Scaffold@MT could potentially enhance regeneration angiogenesis by modulating mitochondrial energy metabolism. Melatonin-loaded gelatin poly-lactic-acid were fabricated electrospinning, then fragmented into shorter fibers. was created through process involving homogenization, low-temperature lyophilization, chemical cross-linking, while maintaining microstructure continuous nanofibers. incorporation short led low release increased Young's modulus scaffold. demonstrated positive biocompatibility promoting 14.2% increase cell proliferation. comparison control group, significantly enhanced matrix mineralization 3.2-fold upregulated gene expression osteoblast-specific markers, thereby facilitating osteogenic differentiation marrow mesenchymal stem cells (BMMSCs). Significantly, marked enhancement function BMMSCs, evidenced elevated adenosine triphosphate (ATP) production, membrane potential, protein respiratory chain factors. Furthermore, promoted migration human umbilical vein endothelial (HUVECs) tube formation 1.3 times compared accompanied an vascular growth factor (VEGFA) expression. results vivo experiments indicate implantation improved vascularized distal femur defect rats. Micro-computed tomography analysis conducted 8 weeks post-surgery revealed optimal development new microarchitecture. Histological immunohistochemical analyses facilitated deposition blood vessel at site. Overall, utilization melatonin-loaded nanofiber sponges exhibits significant promise as promotes angiogenesis, making it viable option repair critical-sized

Language: Английский

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