An Integrating Microfluidic System for Concentration Gradient Generation of Exosomes and Exosome-Assisted Single-Cell-Derived Tumor-Sphere Formation

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

To enhance exploration on tumor stem-like cells (TSCs) without altering their cellular biological characteristics, researchers advocate for application of single-cell-derived tumor-spheres (STSs). TSCs are regulated by surrounding microenvironment, making it crucial to simulate a microenvironment facilitate STS formation. Recently, exosomes that originated from the have emerged as promising approach mimicking microenvironment. In various associated (such fibroblasts, endothelial cells, and immune cells) play roles. Utilizing derived these enabled us promote Herein, we developed an integrated microfluidic platform generate serial concentration gradients evaluate effects multiple demonstrate feasibility our approach, generated two different cell types (HUVEC NIH/3T3 assessed Subsequently, investigated drug resistance STSs treated with free doxorubicin doxorubicin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles. Our findings revealed mixed could be successfully generated, leading enhanced formation rate size STSs. Compared one type, exhibited superior promotion Additionally, nanomedicines demonstrated reduction in compared treatment, particularly smaller and/or more deformable TSCs. This provides innovative enhancement simulation.

Language: Английский

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Numerical investigation on flow regime transition mechanism and length prediction method of droplet in T-junction by electric field

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 149874 - 149874

Published: Feb. 20, 2024

Language: Английский

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Simple and Convenient Three‐Electrode Layout for Real‐Time Electrorotation Measurement Upon Chemical Stimulation

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

ABSTRACT We developed a novel electrorotation (ROT) device featuring microwell array with three electrodes. This allows to monitor the increase in membrane capacitance of cells subjected chemical stimulation. The is integrated into bottom fluidic channel and holds rotating during stimulation solution containing agent. Positive dielectrophoresis (p‐DEP) effectively traps microwells, whereas negative DEP (n‐DEP) facilitates rapid formation single‐cell presence. Alternating current (AC) voltages 120° phase shift applied across electrodes enable vertical simultaneous rotation cells. observed peak rate as function frequency, frequency spectrum shifting lower frequencies increased. A positive correlation was identified between capacitance, so monitoring low‐frequency range advantageous. Although n‐DEP at risks removing from continuous ROT achieved by regulating height center demonstrated induced Ca 2+ influx ionomycin. simple configuration statistical analysis rates without fluorescent labeling, making it suitable for label‐free assessments white blood cells’ responses stimuli.

Language: Английский

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High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(17), P. 6764 - 6773

Published: April 15, 2024

Tremendous efforts have been made to develop practical and efficient microfluidic cell particle sorting systems; however, there are technological limitations in terms of system complexity low operability. Here, we propose a sheath flow generator that can dramatically simplify operational procedures enhance the usability sorters. The device utilizes an embedded polydimethylsiloxane (PDMS) sponge with interconnected micropores, which is direct contact microchannels seamlessly integrated into platform. high-density micropores on surface facilitated fluid drainage, drained was used as for downstream processes. To fabricate device, new process sponge-embedded substrates developed through accumulation, incorporation, dissolution PMMA microparticles sacrificial porogens. effects microchannel geometry velocity generation were investigated. Furthermore, asymmetric lattice-shaped network cell/particle connected series, performances model particles, blood cells, spiked tumor cells technique this study expected streamline conventional cell-sorting systems it improves versatility

Language: Английский

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In-depth Exploration of the Basics of Microfluidic Cell Deformability to Unveil its Applications in the Modern Era

Current Pharmaceutical Design, Journal Year: 2024, Volume and Issue: 30(21), P. 1625 - 1629

Published: May 15, 2024

Language: Английский

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A Microfluidic Impedance Cytometer for Accurate Detection and Counting of Circulating Tumor Cells by Simultaneous Mechanical and Electrical Sensing

2022 IEEE International Symposium on Circuits and Systems (ISCAS), Journal Year: 2024, Volume and Issue: unknown, P. 1 - 5

Published: May 19, 2024

Language: Английский

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Cell elasticity measurement and sorting based on microfluidic techniques: advances and applications

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 116985 - 116985

Published: Nov. 25, 2024

Language: Английский

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Challenges in the processing and preservation of adipose-derived stem cell subpopulations for clinical use

Regenerative Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 3

Published: Dec. 20, 2024

KEYWORDS: Adipose-derived stem cellssubpopulation selectionheterogeneityimmunophenotypespersonalized medicine

Language: Английский

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