Comparative Analysis of Serum and Saliva Iron, Ferritin, and TIBC in Women with Iron Deficiency Anemia: Highlighting Saliva's Screening Potential DOI Open Access
Taraneh Farrokhnia, Reyhaneh Shoorgashti,

Mohammadreza Sadeghian

et al.

Middle East Journal of Rehabilitation and Health Studies, Journal Year: 2024, Volume and Issue: 11(4)

Published: July 21, 2024

Background: Iron deficiency anemia is a prevalent form of anemia, particularly among women. Diagnosing and tracking this condition typically requires various measurements serum iron, ferritin, total iron-binding capacity (TIBC) through blood testing. Objectives: This study aims to evaluate the utility saliva as predictive marker for monitoring iron levels in by comparing correlating TIBC anemic healthy Methods: In comparative analysis, 40 women aged 20 - with similar periodontal status (anemic subjects = 20) were examined. After complete hematological oral examination, salivary samples collected using spitting method, measured compared levels. Data analyzed SPSS 24. Results: The results showed that differences ferritin insignificant between two groups (P-value 0.07 0.26, respectively), but those statistically significant 0.0001 0.0001, respectively). Additionally, difference was found level 0.001), which negligible 0.118). Conclusions: underscore efficacy integrating both analyses comprehending iron-related parameters. While analysis cannot entirely substitute it represents convenient approach may serve an initial step or be employed large-scale screenings.

Language: Английский

Fill, Fold, Photo: Preconcentration and Multiplex Detection of Trace Level Heavy Metals in Water DOI
Prakash Aryal,

Jason Boes,

Eric Brack

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(10), P. 5479 - 5488

Published: Sept. 23, 2024

Heavy metal contamination is an increasing global threat to human and environmental health, particularly in resource-limited areas. Traditional platforms for heavy detection are labor intensive expensive require lab facilities. While paper-based colorimetric sensors offer a simpler approach, their sensitivity limitations prevent them from meeting legislative requirements many metals. Existing preconcentration systems, on the other hand, can achieve lower limits but typically focus analyzing only one metal, making comprehensive monitoring difficult. We address these by introducing low-cost system coupled with analysis simultaneous of seven ions at low ppb levels without need external equipment outside smartphone. The achieved 15 (Ni(II)), 7 (Cu(II)), 2 (Fe(III)), 20 (Cr(VI)), 13 (Pb(II)), 26 (Hg(II)), (Mn(II)) six out values falling well below EPA regulatory guidelines drinking water. user-friendly Fill, Fold, Photo approach eliminates complex pretreatment steps. Smartphone-based offers portable quantification within seconds. Employing masking strategies ensured higher selectivity each assay card, while our packaging protocols enable stability over 4 weeks study, facilitating mass production deployment realistic time frame. To validate sensor's performance real-world scenarios, sensor was tested water samples. demonstrated good recovery, ranging 77% 94% compared standard ICP-MS method. Furthermore, spike recovery confirmed accuracy, relative deviation (RSD) less than 15%. This technology holds significant promise future development as convenient, solution field-based broad spectrum contaminants, including pesticides, PFAS, fertilizers, beyond.

Language: Английский

Citations

7
Capillary-flow driven microfluidic sensor based on tyrosinase for fast user-friendly assessment of pesticide exposures DOI Creative Commons
Claire E. Hefner, Prakash Aryal, Eric Brack

et al.

The Analyst, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Assessment of pesticide exposure using a fast-flow capillary device (created with Bio.Render.com).

Language: Английский

Citations

6
Advancements and challenges in microfluidic paper-based analytical devices: design, manufacturing, sustainability, and field applications DOI Creative Commons
Prakash Aryal, Charles S. Henry

Frontiers in Lab on a Chip Technologies, Journal Year: 2024, Volume and Issue: 3

Published: Dec. 20, 2024

Microfluidic paper-based analytical devices (µPADs) have gained significant attention in science and technology due to their numerous advantages over traditional techniques, including cost-effectiveness, miniaturization, sustainability. µPADs are widely used point-of-care diagnostics, health monitoring, environmental detection, food safety, forensics, security. Since the first report 2007, there been substantial advancements fabrication detection methods, applications. Over years, efforts made improve manufacturing scalability, user-friendliness, sustainability of µPADs. In this review, we explore general trends fabrication, sensing applications µPADs, highlighting key improvements innovations. We also examine eco-friendliness present real-world success stories from field studies citizen initiatives. Additionally, address challenges associated with transitioning lab field. Finally, outlook for propose strategies usefulness various fields.

Language: Английский

Citations

6
Advanced Microfluidic‐Based Wearable Electrochemical Sensors for Continuous Biochemical Monitoring DOI Creative Commons

Sehyun Park,

Seongyeop Kim,

Soo-Jin Lee

et al.

Advanced Electronic Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Abstract Microfluidic‐based wearable electrochemical sensors represent a transformative approach to non‐invasive, real‐time health monitoring through continuous biochemical analysis of body fluids such as sweat, saliva, and interstitial fluid. These systems offer significant potential for personalized healthcare disease management by enabling detection key biomarkers. However, challenges remain in optimizing microfluidic channel design, ensuring consistent biofluid collection, balancing high‐resolution fabrication with scalability, integrating flexible biocompatible materials, establishing standardized validation protocols. This review explores advancements techniques, integrated that have improved sensitivity, selectivity, durability. Conventional photolithography, 3D printing, laser‐based methods are compared, highlighting their mechanisms, advantages, trade‐offs production. The application section summarizes strategies overcome variability composition, sensor drift, user adaptability innovative solutions hybrid material integration, self‐powered systems, AI‐assisted data analysis. By analyzing recent breakthroughs, this paper outlines critical pathways expanding technologies achieving seamless operation diverse real‐world settings, paving the way new era digital health.

Language: Английский

Citations

0
Smartphone-Assisted Dual-Sided Capillary Microfluidic Device for Multiplex Detection of Heavy Metals and Nutrients in Drinking Water DOI

Pornchanok Punnoy,

Prakash Aryal, Claire E. Hefner

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1356, P. 344031 - 344031

Published: April 10, 2025

Language: Английский

Citations

0
Citizen-Based Water Quality Monitoring: Field Testing a User-Friendly Sensor for Phosphate Detection in Global Surface Waters DOI
Prakash Aryal, Claire E. Hefner, Brandaise Martinez

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(46), P. 18369 - 18376

Published: Nov. 1, 2024

Widespread concern over surface water pollution has led to interest in developing easy-to-use accurate tools for citizen-based measurements that provide high spatial and temporal resolution while maintaining accuracy. Excessive anthropogenic phosphate significantly contributes global eutrophication necessitates regular on-site monitoring waters. Traditional instrumentation quantifying is labor-intensive, expensive, performed laboratories. Existing testing methods relying on phosphomolybdenum blue (PMB) have limited sensitivity stability under ambient conditions. To overcome these limitations, a novel low-cost, rapid, user-friendly sensor citizen-led introduced demonstrated with sampling campaign. The fast-flow microfluidic device provides operation, achieving an environmentally relevant limit of detection (LoD) 190 ppb, which near the EPA-recommended maximum phosphate. dip-and-read operation reduces procedural steps delivering sample volume, making it well-suited science initiatives. This exhibits selectivity prolonged two months sensor's performance was validated using industry-standard UV–Vis method 90% correlation. More than 1000 sensors were deployed different continents, facilitating mapping diverse sources across multiple continents. initiative covered much globe, including Thailand, Nepal, Brazil, Chile, USA, Germany. In some cases, levels exceeded legislative guidelines by 100-fold. Through collaboration citizen scientists, we analyzed regional topography socioeconomic practices sources, identifying potential could contribute areas.

Language: Английский

Citations

2
Comparative Analysis of Serum and Saliva Iron, Ferritin, and TIBC in Women with Iron Deficiency Anemia: Highlighting Saliva's Screening Potential DOI Open Access
Taraneh Farrokhnia, Reyhaneh Shoorgashti,

Mohammadreza Sadeghian

et al.

Middle East Journal of Rehabilitation and Health Studies, Journal Year: 2024, Volume and Issue: 11(4)

Published: July 21, 2024

Background: Iron deficiency anemia is a prevalent form of anemia, particularly among women. Diagnosing and tracking this condition typically requires various measurements serum iron, ferritin, total iron-binding capacity (TIBC) through blood testing. Objectives: This study aims to evaluate the utility saliva as predictive marker for monitoring iron levels in by comparing correlating TIBC anemic healthy Methods: In comparative analysis, 40 women aged 20 - with similar periodontal status (anemic subjects = 20) were examined. After complete hematological oral examination, salivary samples collected using spitting method, measured compared levels. Data analyzed SPSS 24. Results: The results showed that differences ferritin insignificant between two groups (P-value 0.07 0.26, respectively), but those statistically significant 0.0001 0.0001, respectively). Additionally, difference was found level 0.001), which negligible 0.118). Conclusions: underscore efficacy integrating both analyses comprehending iron-related parameters. While analysis cannot entirely substitute it represents convenient approach may serve an initial step or be employed large-scale screenings.

Language: Английский

Citations

0