Biomaterials Science, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
A novel IL-based delivery system effectively delivered siBcl-2 into melanoma cells and led to significant apoptosis both in vitro vivo , providing compelling evidence for remarkable antitumor effects.
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1739 - 1739
Published: Feb. 1, 2024
Single-stranded messenger ribonucleic acid (mRNA) plays a pivotal role in transferring genetic information, and tremendous effort has been devoted over the years to utilize its transcription efficacy therapeutic interventions for variety of diseases with high morbidity mortality. Lipid nanocarriers have extensively investigated mRNA delivery enabled rapid successful development vaccines against SARS-CoV-2. Some constraints lipid encouraged alternative systems, such as polymer-based soft nanoparticles, which offer modular gene platform. Such macromolecule-based can be synthetically articulated tailored parameters including protection, loading efficacy, targeted release. In this review, we highlight recent advances polymeric architectures delivery, their limitations, challenges that still exist, aim expediting further research clinical translation formulations.
Language: Английский
Citations
16
International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 25 - 52
Published: Jan. 1, 2025
Abstract: The effective clinical translation of messenger RNA (mRNA), small interfering (siRNA), and microRNA (miRNA) for therapeutic purposes hinges on the development efficient delivery systems. Key challenges include their susceptibility to degradation, limited cellular uptake, inefficient intracellular release. Polymeric drug conjugates (PDCs) offer a promising solution, combining benefits polymeric carriers agents targeted treatment. This comprehensive review explores nucleic acid therapeutics, focusing conjugates. It investigates how these address obstacles, enhance systemic circulation, reduce immunogenicity, provide controlled release, improving safety profiles. delves into conjugation strategies, preparation methods, various classes PDCs, as well strategic design, highlighting role in delivery. Applications PDCs treating diseases such cancer, immune disorders, fibrosis are also discussed. Despite significant advancements, adoption persist. concludes with insights future directions this transformative technology, underscoring potential advance acid-based therapies combat infectious significantly. Keywords: polymer conjugates,
Language: Английский
Citations
1
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 7099 - 7121
Published: July 1, 2024
Abstract: Introduction of exogenous genes into target cells to overcome various tumor diseases caused by genetic defects or abnormalities and gene therapy, a new treatment method, provides promising strategy for treatment. Over the past decade, therapy has made exciting progress; however, it still faces challenge low nucleic acid delivery release efficiencies. The emergence nonviral vectors, primarily nanodelivery systems (NDRS), resulted in historic breakthrough application therapy. NDRS, especially stimulus-responsive NDRS that can respond timely manner changes internal external microenvironment (eg, pH, high concentration glutathione/reactive oxygen species, overexpressed enzymes, temperature, light, ultrasound, magnetic field), shown excellent loading advantages precision efficiency been widely applied. only disadvantage is poor transfection limits in-depth clinical practice, owing presence biological barriers body. Therefore, this review first introduces development history current obstacles faced delivery, strategies these obstacles, conventional then focuses on latest research progress improving efficiency. Finally, future challenges prospects may face transformation are discussed provide references enhancing Keywords: treatment, efficiency, microenvironment, physiological barrier, efficacy improvement
Language: Английский
Citations
5
Journal of Functional Biomaterials, Journal Year: 2024, Volume and Issue: 15(11), P. 324 - 324
Published: Oct. 31, 2024
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats associated with protein 9) was first identified as a component of the bacterial adaptive immune system and subsequently engineered into genome-editing tool. The key breakthrough in this field came realization that could be used mammalian cells to enable transformative genetic editing. This technology has since become vital tool for various manipulations, including gene knockouts, knock-in point mutations, regulation at both transcriptional post-transcriptional levels. holds great potential human medicine, particularly curing disorders. However, despite significant innovation advancement genome editing, still possesses critical limitations, such off-target effects, immunogenicity issues, ethical considerations, regulatory hurdles, need efficient delivery methods. To overcome these obstacles, efforts have focused on creating more accurate reliable Cas9 nucleases exploring innovative Recently, functional biomaterials synthetic carriers shown effective vehicles components. In review, we attempt provide comprehensive survey existing CRISPR-Cas9 strategies, viral delivery, biomaterials-based carriers, physical techniques. We underscore urgent systems fully unlock power realize seamless transition from benchtop research clinical applications.
Language: Английский
Citations
5
Polymer Chemistry, Journal Year: 2024, Volume and Issue: 15(34), P. 3436 - 3468
Published: Jan. 1, 2024
Lipid–polymer hybrid nanoparticles are rapidly emerging as a major class of efficient delivery systems for biomedical applications. This review showcases and discusses the designs advances lipid–polymer hybrids genome editing strategies.
Language: Английский
Citations
3
Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(2)
Published: Sept. 24, 2023
Mitochondrial potassium ion channels have become a promising target for cancer therapy. However, in malignant tumors, their low expression or inhibitory regulation typically leads to undesired therapy, even induces drug resistance. Herein, this work develops an situ mitochondria-targeted artificial K
Language: Английский
Citations
9
Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 110282 - 110282
Published: July 1, 2024
Language: Английский
Citations
1
Advances in Natural Sciences Nanoscience and Nanotechnology, Journal Year: 2024, Volume and Issue: 15(4), P. 045003 - 045003
Published: Sept. 6, 2024
Abstract Purpose . Gene therapy is a strategy to provide therapeutic advantages by modifying genes, and CRISPR/Cas9 the simplest most efficient gene editing technology. Appropriate smart nanocarriers are promising way deliver these tools into cells efficiently. Methods In this study, three novel were prepared for delivery PC12 cells. We designed polyplex using synthetic redox-responsive polyethyleneimine (rPEI) made crosslinked PEI ∼ 2 KD pCRISPR electrostatic interactions. Then, avoid unwanted interactions with blood ingredients natural polysaccharides negative charge, hyaluronic acid (HA), chondroitin sulfate (CS), alginate(ALG) have been used separately as outer shells. Additionally, characterized in terms of zeta potential, size distribution, loading efficiency. Finally, cytotoxicity GFP expression evaluated. Results The average coats HA, ALG, CS was around 47, 66.5, 309 nm, respectively. Furthermore, indicated high efficiency, capacity cellular uptake (>90%), no significant toxicity. amount estimated up 21%. Conclusion These polymeric suggest carriers delivery.
Language: Английский
Citations
1
Biomaterials Science, Journal Year: 2024, Volume and Issue: 12(12), P. 3068 - 3085
Published: Jan. 1, 2024
Non-viral vector-based CRISPR-Cas-mediated immunoengineering in tumor cells and immune for cancer immunotherapy.
Language: Английский
Citations
0
Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 25, 2024
Intracellular delivery of proteins has attracted significant interest in biological research and cancer treatment, yet it continues to face challenges due the lack effective approaches. Herein, we developed an efficient strategy via cationic α-helical polypeptide-mediated anionic proprotein delivery. The protein was reversibly modified with adenosine triphosphate dynamic covalent chemistry prepare (A-protein) abundant phosphate groups. A guanidyl-decorated polypeptide (LPP) employed not only encapsulate A-protein through electrostatic attraction hydrogen bonding, forming stable nanocomplexes, but also enhance cell membrane penetration its rigid conformation. Consequently, this mediated various different isoelectric points molecular weights, including α-chymotrypsin, bovine serum albumin, ribonuclease A, cytochrome C, saporin, horseradish peroxidase, β-galactosidase, anti-phospho-Akt, into cells. More importantly, enabled CRISPR-Cas9 ribonucleoproteins elicit robust polo-like kinase 1 genome editing for inhibiting growth. This rationally designed system may benefit development intracellular protein-based therapy.
Language: Английский
Citations
0