Optimizing locally delivered periodontitis therapy: Development of chitosan‐hydroxyapatite‐encapsulated drug via electrospraying DOI
Binpeng Zhang,

Shaofu Yan,

Yao Zhang

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 23, 2024

Abstract This study presents the development of an optimized drug delivery system for periodontitis treatment utilizing chitosan‐hydroxyapatite nanoparticles. Employing electrospraying technique, researchers encapsulated antibiotic amoxicillin within composite matrices while varying concentrations chitosan and hydroxyapatite The resulting microparticles displayed sizes ranging from 276 to 386 μm. Characterization produced encapsulations indicated that higher resulted in formation larger particles with rougher surfaces, increased mechanical strength, enhanced thermal stability. Notably, nanoindentation analysis revealed increase hardness 0.21 0.35 GPa, elastic modulus 5.32 8.72 GPa escalating content, meeting or surpassing requirements load‐bearing regenerative biomaterials. In vitro release assessments exhibited sustained over 24 h, predominantly through diffusion dissolution mechanisms. Formulations lower polymer mineral content showcased elevated rates, F1 encapsulation (0.5% chitosan, 0.2% HAP) achieving a cumulative 78.3 ± 3.2% contrast 65.1 2.7% F5 formulation (2.5% 1% HAP). These selectively modulated overproduction proinflammatory cytokines, including IL‐1β, IL‐6, TNF‐α, gingival fibroblasts osteoblasts without compromising cell viability. An artificial neural network (ANN) model accurately forecasted material properties biological performance formulations based on their compositional variations, yielding correlation coefficients exceeding 0.97. computational platform offers virtual screening tool refining therapies. developed microparticulate demonstrates promising potential prolonged controlled delivery, improved properties, targeted regulation inflammatory response.

Language: Английский

Physical strategies to engineer supramolecular composite hydrogels for advanced biomedical applications DOI

Sravan Baddi,

Auphedeous Y. Dang-i,

Fengli Gao

et al.

Progress in Materials Science, Journal Year: 2025, Volume and Issue: 151, P. 101428 - 101428

Published: Jan. 9, 2025

Language: Английский

Citations

4
A comprehensive review on recent progress in chitosan composite gels for biomedical uses DOI
Smita Bonde, Chandni Chandarana, Parixit Prajapati

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 272, P. 132723 - 132723

Published: May 31, 2024

Language: Английский

Citations

13
3D-Printed Chitosan-Based Scaffolds with Scutellariae baicalensis Extract for Dental Applications DOI Creative Commons
Magdalena Paczkowska-Walendowska, Ioanna Koumentakou, Maria Lazaridou

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(3), P. 359 - 359

Published: March 4, 2024

The plant material Scutellariae baicalensis radix, which is rich in flavones (baicalin), possesses antibacterial, antifungal, antioxidant, and anti-inflammatory properties. This work aimed to develop a 3D-printed chitosan-based hydrogel extract as an innovative approach for the personalized treatment of periodontal diseases. Chitosan-based hydrogels were prepared, printability prepared was determined. with 2.5% w/v high molecular-weight chitosan (CS), 2% gelatin (Gel), 10% w/w (Ex) presented best printability, producing smooth uniform scaffolds. It proved that CS/Gel/Ex stabilized by hydrogen bonds remained amorphous dispersion structures (confirmed ATR-FTIR XRPD). Due amorphization active substance, significant increase release baicalin vitro observed. demonstrated there initial burst continuous profile (n = 3). Higuchi kinetic most likely kinetic. second fit, Korsmeyer–Peppas kinetics model, showed coupled diffusion ingredient hydrated matrix polymer relaxation regulated release, n values ranging from 0.45 0.89. properties scaffolds assessed ability inhibit activity hyaluronidase enzyme. Activity IC50 63.57 ± 4.98 mg hydrogel/mL 6). Cytotoxicity tests biocompatibility material. After 24 h exposure 2.5CS/2Gel/10Ex scaffold, fibroblasts migrated toward scratch, closed “wound” 97.1%, significantly accelerated wound healing process. results render CS/Gel/extract potential candidates treating

Language: Английский

Citations

11
Dual-functional Hydroxyapatite scaffolds for bone regeneration and precision drug delivery DOI
Ashkan Farazin, Soroush Mahjoubi

Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials, Journal Year: 2024, Volume and Issue: 157, P. 106661 - 106661

Published: July 14, 2024

Language: Английский

Citations

11
Biofabrication Approaches for Peri-Implantitis Tissue Regeneration: A Focus on Bioprinting Methods DOI Open Access
Dobromira Shopova, Anna Mihaylova, Antoniya Yaneva

et al.

Prosthesis, Journal Year: 2024, Volume and Issue: 6(2), P. 372 - 392

Published: April 16, 2024

Dental implant utilization has emerged as a contemporary strategy for rectifying dental arch anomalies. However, the effective management of potential complications is paramount. Peri-implantitis, characterized by inflammation and bone resorption around implants, resembles periodontitis but specifically affects sites. Restoring lost peri-implant tissues poses multifaceted challenge, with bioprinting methods showing promise viable solution. Three-dimensional represents forefront advancement in tissue engineering, traditionally focusing on scaffolds, cells, signaling pathways. This systematic review aims to aggregate synthesize data concerning bioprinting’s application peri-implantitis treatment. Adhering PRISMA guidelines, conducted an extensive literature search across PubMed, Scopus, Google Scholar, ScienceDirect. Importantly, timeframe was not limited, reflecting scarcity available information subject. Bioprinting advancements offer auspicious avenues refining treatment modalities, prompting clinicians explore optimal solutions establishing ideal anatomical conditions. In essence, this underscores 3D management, highlighting its pivotal role medicine capacity reshape clinical approaches toward achieving outcomes.

Language: Английский

Citations

5
Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering DOI Creative Commons
Deniz Atila, Ali Deniz Dalgıç, Agnieszka Krzemińska

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

An injectable hydrogel formulation is developed utilizing low- and high-molecular-weight chitosan (LCH HCH) incorporated with curcumin α-tocopherol-loaded liposomes (Lip/Cur+Toc). Cur Toc releases are delayed within the hydrogels. The injectability of hydrogels proved via rheological analyses. In vitro studies conducted using human dental pulp stem cells (hDPSCs) gingival fibroblasts (hGFs) to examine biological performance toward endodontics periodontics, respectively. viability hDPSCs treated Lip/Cur+Toc highest till day 14, compared neat During odontogenic differentiation tests, alkaline phosphatase (ALP) enzyme activity induced in Cur-containing groups. Biomineralization enhanced mostly incorporation. hGFs HCH combined while wound healing occurs almost 100% both (Lip/Cur+Toc@LCH Lip/Cur+Toc@HCH) after 2 days. Antioxidant Lip/Cur+Toc@LCH on significantly among Antimicrobial tests demonstrate that more effective against Escherichia coli whereas so Lip/Cur+Toc@HCH Staphylococcus aureus. antimicrobial mechanism investigated for first time through various computational models. LCH loaded promising candidates multi-functional features periodontics.

Language: Английский

Citations

5
Injectable gelatin-pectin hydrogel for dental tissue engineering: Enhanced angiogenesis and antibacterial efficacy for pulpitis therapy DOI

Chau My Phan,

Cuong Hung Luu, Mohanapriya Murugesan

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137939 - 137939

Published: Nov. 1, 2024

Language: Английский

Citations

5
Guidance on biomaterials for periodontal tissue regeneration: Fabrication methods, materials and biological considerations DOI Creative Commons
Nicholas G. Fischer, Isaac Jordão de Souza Araújo, Arwa Daghrery

et al.

Dental Materials, Journal Year: 2025, Volume and Issue: 41(3), P. 283 - 305

Published: Jan. 9, 2025

Regeneration of the multiple tissues and interfaces in periodontal complex necessitates multidisciplinary evaluation to establish structure/function relationships. This article, an initiative Academy Dental Materials, provides guidance for performing chemical, structural, mechanical characterization materials tissue regeneration, outlines important recommendations on methods testing bioactivity, biocompatibility, antimicrobial properties biomaterials/scaffolds engineering. First, we briefly summarize engineering fabrication methods. We then highlight critical variables consider when evaluating a material fundamental tests used investigate them. The recommended designs incorporate relevant international standards provide framework characterizing newly developed focusing applicability those regeneration. most common biofabrication (electrospinning, injectable hydrogels, fused deposition modelling, melt electrowriting, bioprinting) their specific applications are reviewed. techniques morphological, different classes regeneration described. major advantages drawbacks each assay, sample sizes, guidelines specimen preparation also highlighted. From biological standpoint, biocompatibility materials, experimental models potential included this guidance. In conclusion, researchers studies will have as tool assess essential characteristics materials/scaffold-based strategies.

Language: Английский

Citations

0
Cytotoxic and Antibiofilm Properties of Antibiotic-Loaded Thermoresponsive Hydrogels for Root Canal Therapy DOI Open Access
Cristiane Duque, Gabriela Pacheco de Almeida Braga, Juliana Machado de Carvalho

et al.

Processes, Journal Year: 2025, Volume and Issue: 13(3), P. 661 - 661

Published: Feb. 26, 2025

Dental infections can disrupt root development in immature permanent teeth, making traditional endodontic treatment challenging. Apexogenesis, a regenerative approach that promotes natural development, offers potential solution. However, issues related to disinfection and material biocompatibility still remain. The objective of this study was evaluate the synergistic antimicrobial antibiofilm properties double triple antibiotic combinations against common oral pathogens, incorporate most effective combination into thermosensitive hydrogel, develop an alternative intracanal medication. Antibiotics were tested alone planktonic biofilm conditions bacteria Candida albicans. with synergy on Enterococcus faecalis biofilms radicular dentin by confocal microscopy. Metronidazole (ME), ciprofloxacin (CI), fosfomycin (FO) incorporated poly(N-vinylcaprolactam) (PNVCL) hydrogels, their activity compared PNVCL hydrogels containing chlorhexidine (CHX) or calcium hydroxide (CH). cytotoxicity assessed MDPC-23 odontoblast-like cells using metiltetrazolium assays. A statistical analysis performed ANOVA followed Tukey’s test (p < 0.05). ME + CI FO showed superior effects mono- dual-species inside dentinal tubules, comparable CHX. significantly reduced E. exhibiting higher efficacy than CH. Cytotoxicity tests revealed minimal cell viability for both without antibiotics. In conclusion, potent and, when loaded demonstrated significant low cytotoxicity. These findings emphasize formulation as biocompatible medication, especially teeth.

Language: Английский

Citations

0
Futuristic Dentistry: Towards Medical–Dental Integration and Minimalistic Approaches DOI

Andrea Lorena Basualdo Allende

Published: Jan. 1, 2025

Language: Английский

Citations

0