Macrophage Membrane-Coated Nanoparticles for the Delivery of Natamycin Exhibit Increased Antifungal and Anti-Inflammatory Activities in Fungal Keratitis DOI
Xing Liu, Yunfeng Zhang, Fang Peng

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(44), P. 59777 - 59788

Published: Oct. 28, 2024

This study aims to explore the efficacy and safety of macrophage membrane-coated nanoparticles for delivery natamycin (NAT) in therapy fungal keratitis (FK). Macrophage membranes were isolated identified by immunofluorescence staining (IFS). NAT was encapsulated into poly(lactic-co-glycolic acid) (PLGA). Fungal stimulated (M1) or unstimulated (M) separately mixed sonicated with PLGA nanoparticles. The biocompatible (PLGA-NAT, PLGA-NAT@M, PLGA-NAT@M1) characterized zeta-sizer analysis, transmission electron microscopy (TEM), Western blot. Drug encapsulation loading efficiency release detected ultraviolet spectrophotometry. cytotoxicity, ocular surface toxicity irritability, systemic different concentrations assessed. In vitro, we examined antifungal properties eye retention time, drug release, curative effects on FK evaluated vitro vivo. IFS results showed separation membrane nucleus. prepared had a typical "core–shell" structure uniform nanometer size, proteins retained allowing exert functional macrophage. efficiencies PLGA-NAT@M PLGA-NAT@M1 7.6 6.7%, respectively. 51.2 41.5%, could gradually reduce clearance surface. enhanced activity PLGA-NAT. Furthermore, coated increased biocompatibility decreased corneal vivo, significantly alleviated severity FK. PLGA@M PLGA@M1 reduced protein levels inflammatory cytokines after stimulation. has good physical biosafety. It evade clearance, gradually, achieve high anti-inflammatory clinically have application potential treatment

Language: Английский

The Emerging Role of Cell Membrane-coated Nanomaterials in Cancer Therapy DOI
Sankha Bhattacharya, Paul Beninger

Current Pharmaceutical Design, Journal Year: 2024, Volume and Issue: 30(10), P. 727 - 741

Published: March 1, 2024

Abstract: This review investigates the revolutionary application of cell membrane-coated nanoparticles (CMNPs) as a promising avenue for cancer therapy within embryonic landscape nanotechnology. Nanoparticles, pivotal in treatment, are systematically examined their diverse physicochemical structures, categorized organic (lipid-based, protein-based, and polymer-assisted) inorganic (carbon-based metal) varieties. A significant focus is placed on CMNPs, which serve an innovative drug delivery vehicle, overcoming limitations associated with conventional nanoparticle therapies. manuscript accurately explores advantages challenges various membranes, including those derived from cells, red blood platelets, stem white cells. Importance roles enhancing precision, immune system circumvention, targeted recognition. Detailed insights into crafting CMNPs provided, elucidating membrane extraction fusion techniques, such sonication, extrusion, co-extrusion, microfluidic electroporation. Maintaining integrity during benefits coating techniques augmenting biocompatibility underscored. comprehensive resource consolidates latest advancements delivery, positioning itself at forefront nanotechnology biomedicine research. Encapsulating methodologies like electrospray, chemical conjugation, this showcases expanding toolbox available to researchers dynamic field. Focusing unique characteristics multifaceted applications biomedical research, particularly tumour therapy. It provides indepth analysis stability, evasion capabilities, increased payload capacity, retained biological functionality. The outlines current future prospects chemotherapy, photothermal photodynamic therapy, immunotherapy, gene therapeutic methods. concludes by highlighting transformative potential reshaping treatment.

Language: Английский

Citations

2
ACYP2 functions as an innovative nano-therapeutic target to impede the progression of hepatocellular carcinoma by inhibiting the activity of TERT and the KCNN4/ERK pathway DOI Creative Commons
Yixuan Wu,

Hongyi Bao,

Jinran Wu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 12, 2024

An increasing body of evidence suggests that acylphosphatase-2 (ACYP2) polymorphisms are correlated with an increased susceptibility to a range malignancies. Nevertheless, its potential functions, molecular mechanisms in hepatocellular carcinoma (HCC) and whether it can be act as therapeutic target remain uninvestigated. Herein, ACYP2 was found lowly expressed HCC negatively tumor size, differentiation, microvascular invasion the prognosis patients. Functional investigations revealed overexpression inhibited proliferation metastasis cells while promoting apoptosis; knockdown had exact opposite effect. Additionally, observed distributed both cytoplasm nucleus cells. According mechanistic studies, expression potassium calcium-activated channel subfamily N member 4 (KCNN4) regulated by cytoplasmic ACYP2, resulting inhibition K

Language: Английский

Citations

2
Electrostatic attachment of exosome onto a 3D-fabricated calcium silicate/polycaprolactone for enhanced bone regeneration DOI Creative Commons

Ju Hyun Yun,

Hyeyoung Lee, Se Hyun Yeou

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 29, P. 101283 - 101283

Published: Oct. 1, 2024

Language: Английский

Citations

2
Macrophage Membrane-Coated Nanoparticles for the Delivery of Natamycin Exhibit Increased Antifungal and Anti-Inflammatory Activities in Fungal Keratitis DOI
Xing Liu, Yunfeng Zhang, Fang Peng

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(44), P. 59777 - 59788

Published: Oct. 28, 2024

This study aims to explore the efficacy and safety of macrophage membrane-coated nanoparticles for delivery natamycin (NAT) in therapy fungal keratitis (FK). Macrophage membranes were isolated identified by immunofluorescence staining (IFS). NAT was encapsulated into poly(lactic-co-glycolic acid) (PLGA). Fungal stimulated (M1) or unstimulated (M) separately mixed sonicated with PLGA nanoparticles. The biocompatible (PLGA-NAT, PLGA-NAT@M, PLGA-NAT@M1) characterized zeta-sizer analysis, transmission electron microscopy (TEM), Western blot. Drug encapsulation loading efficiency release detected ultraviolet spectrophotometry. cytotoxicity, ocular surface toxicity irritability, systemic different concentrations assessed. In vitro, we examined antifungal properties eye retention time, drug release, curative effects on FK evaluated vitro vivo. IFS results showed separation membrane nucleus. prepared had a typical "core–shell" structure uniform nanometer size, proteins retained allowing exert functional macrophage. efficiencies PLGA-NAT@M PLGA-NAT@M1 7.6 6.7%, respectively. 51.2 41.5%, could gradually reduce clearance surface. enhanced activity PLGA-NAT. Furthermore, coated increased biocompatibility decreased corneal vivo, significantly alleviated severity FK. PLGA@M PLGA@M1 reduced protein levels inflammatory cytokines after stimulation. has good physical biosafety. It evade clearance, gradually, achieve high anti-inflammatory clinically have application potential treatment

Language: Английский

Citations

2