Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
17(1), P. 7 - 7
Published: Dec. 24, 2024
Background:
Traumatic
hemorrhage
and
infection
are
major
causes
of
mortality
in
wounds
caused
by
battlefield
injuries,
hospital
procedures,
traffic
accidents.
Developing
a
multifunctional
nano-drug
capable
simultaneously
controlling
bleeding,
preventing
infection,
promoting
wound
healing
is
critical.
This
study
aimed
to
design
evaluate
nanoparticle-based
solution
address
these
challenges
effectively.
Methods:
Using
one-pot
assembly
approach,
we
prepared
series
nanoparticles
composed
poly-L-lysine
hyodeoxycholic
acid
(PLL-HDCA
NPs).
Theoretical
simulations
experimental
studies
were
combined
optimize
their
structure
functionality.
In
vitro
platelet
aggregation,
antibacterial
assays,
cytotoxicity
tests,
hemolysis
evaluations
performed.
vivo
efficacy
was
assessed
various
models,
full-thickness
skin
defect
model,
irritation
test.
Results:
PLL-HDCA
NPs
demonstrated
effective
induction
aggregation
significantly
reduced
bleeding
time
blood
loss
mouse
including
tail
vein,
femoral
artery,
liver
bleeding.
Antibacterial
assays
revealed
strong
activity
against
E.
coli
S.
aureus.
Wound
showed
that
promoted
tissue
repair
model.
Cytotoxicity
tests
indicated
minimal
impact
on
human
cells
rates
compared
PLL
alone.
Skin
confirmed
the
safety
for
external
application.
Conclusions:
represent
safe,
efficient,
suitable
topical
applications
control
combat
facilitate
healing,
making
them
promising
candidates
use
settings.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 21, 2025
Dentin
hypersensitivity
caused
by
the
exposure
of
dentinal
tubules
is
affecting
a
significant
portion
population.
With
promising
prospects,
biomimetic
mineralization
materials
used
in
treating
dentin
are
expected
to
possess
metastable
characteristic,
for
which
they
can
easily
penetrate
and
surrounding
tissues,
but
then
occlude
them
via
transformation
size
phase
immediately.
Herein,
this
study
develops
calcium
phosphate
cluster
(MCPC)-involved
process,
regulated
dual
biological
macromolecules:
bovine
serum
albumin
(BSA)
poly-L-lysine
(PLL).
BSA
functions
stabilize
primary
clusters;
PLL
further
tunes
cluster's
evolution
(toward
larger
crystalline
particles)
into
fashion,
meanwhile
inhibits
local
bacteria.
Upon
treatments,
system
generates
amorphous
MCPC
with
ultrasmall
(1-2
nm);
enter
deep
tubules,
subsequently
aggregate
immobile
particles,
finally
seal
exposed
tubules.
The
effective
occlusion
as
well
antibacterial
performance
confirmed
both
vivo
vitro.
This
has
devised
not
only
regulatory
approach
mineralization-active
clusters
also
established
an
efficient
method
managing
hypersensitivity.
Hydrogel
wound
dressings
have
emerged
as
a
promising
solution
for
healing
due
to
their
excellent
mechanical
and
biochemical
properties.
Over
recent
years,
there
has
been
significant
progress
in
expanding
the
variety
of
raw
materials
used
hydrogel
formulation
along
with
development
advanced
modification
techniques
design
approaches
that
enhance
performance.
However,
comprehensive
review
encompassing
diverse
polymer
strategies
innovations
is
still
lacking
literature.
This
summarizes
use
natural
polymers
(e.g.,
chitosan,
gelatin,
sodium
alginate,
hyaluronic
acid,
dextran)
synthetic
poly(vinyl
alcohol),
polyethylene
glycol,
Pluronic
F-127,
poly(N-isopropylacrylamide),
polyacrylamide,
polypeptides)
dressings.
We
further
explore
advantages
limitations
these
discuss
various
strategies,
including
cationic
modification,
oxidative
double-bond
catechol
etc.
The
also
addresses
principles
synthesis
methods,
aligning
modifications
specific
requirements
healing.
Finally,
we
future
challenges
opportunities
hydrogel-based
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2025,
Volume and Issue:
113(1)
Published: Jan. 1, 2025
ABSTRACT
Massive
bleeding
and
bacterial
infection
of
wounds
may
be
life‐threatening
or
even
lead
to
death.
Nowadays,
gelatin‐based
hemostatic
sponges
have
been
widely
used,
but
gelatin
is
not
antibacterial
has
poor
structural
stability.
In
this
study,
we
mixed
an
polypeptide,
ε‐poly‐L‐lysine
(EPL),
into
gelatin.
A
gelatin/ε‐poly‐L‐lysine
(Gel/EPL)
sponge
with
functions
was
prepared
by
ultraviolet
(UV)
crosslinking
lyophilized
Gel/EPL
composite.
Scanning
electron
microscopy
showed
that
the
had
interconnected
porous
structure.
The
incorporation
EPL
increased
hydrophilicity
water
absorption
capacity
sponge.
better
stability
after
UV
crosslinking.
assay
bacteria
could
grow
normally
around
contact
between
blood
components
initiated
coagulation
via
exogenous
pathway
activation,
no
hemolysis
occurred.
addition,
in
vivo
experiments
confirmed
a
faster
clotting
time
lower
loss.
These
findings
show
developed
great
potential
as
novel
agent
can
quickly
stop
fight
infections.
Hydrogels,
particularly
multifunctional
hydrogels,
are
attractive
wound
dressing
due
to
their
abundant
exudate
absorption
performance,
oxygen
permeability,
comparable
moisturizing
ability,
and
performance
of
locally
delivering
therapeutic
drugs.
However,
it
is
challenging
achieve
hydrogels
that
enable
mechanical
properties
human
skin
can
uniformly
load
hydrophobic
drugs
as
dressings
for
management.
Herein,
we
report
a
simple
rapid
fabrication
by
directly
inducing
sericin
protein
gelation
using
acetic
acid.
The
resulting
demonstrate
high
transparency,
excellent
physicochemical
biological
properties,
promising
materials.
Notably,
this
hydrogel
system
could
encapsulate
during
gelation,
thus
overcoming
the
limitation
inadequate
amount
non-uniform
loading
in
hydrogels.
Further,
selectively
encapsulated
ciprofloxacin
(CIP)
into
obtain
with
favorable
antimicrobial
activities.
obtained
CIP-loaded
exhibited
antibacterial
activity
against
both
Gram-positive
bacteria
(S.
aureus)
Gram-negative
(P.
aeruginosa)
vitro.
vivo
studies
demonstrated
effectively
eliminated
wounds
significantly
accelerated
healing.
Taken
together,
potentially
material
Biomaterials Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
Massive
hemorrhage
and
chronic
wounds
caused
by
bacterial
infections
after
trauma
are
significant
challenges
in
clinical
practice.
An
ideal
hemostatic
wound
dressing
should
simultaneously
manage
bleeding
prevent
also
hold
excellent
biocompatibility
bioactivities
to
successfully
modulate
immune
microenvironments
promote
healing.
In
this
study,
a
silk
fibroin-based
light-responsive
film
was
demonstrated
possess
effective
capacity
of
light-induced
non-compressible
hemostasis
on
liver
tail
