Bioactive Zn–V–Si–Ca Glass Nanoparticle Hydrogel Microneedles with Antimicrobial and Antioxidant Properties for Bone Regeneration in Diabetic Periodontitis

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Periodontitis is a chronic inflammatory condition affecting the periodontal tissue. This worsens in diabetic patients due to oxidative stress and inflammation. Herein, we investigated treatment using bioactive Zn–V–Si–Ca glass nanoparticle hydrogel microneedles. The microneedles contain nanoparticles codoped with zinc vanadium ions. They also include gallic acid oxidized methacrylated hyaluronic acid. These address bacterial dysbiosis periodontitis. provide antibacterial antioxidant effects. deliver therapeutic agents directly into gingival enhances drug retention absorption by penetrating mucosal barrier. In vitro studies demonstrated biocompatibility, excellent properties, acceptable mechanical properties. Meanwhile, microneedle patches properties effective against Gram-negative pathogen as well Gram-positive oral bacterium. vivo experiments were performed rat model Results showed significant improvement alveolar bone regeneration. modulated microenvironment effectively. Ribonucleic sequencing revealed downregulation of JAK-STAT NF-κB inflammation signaling pathways. work presents distinctive approach suppressing response modulate immune responses for purpose treating periodontitis early.

Language: Английский

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Dual-Pathway Targeted Therapy for Parkinson's Disease: Biomimetic Nanosomes Inhibit Ferroptosis and Pyroptosis Through NLRP3 Inflammasome Regulation

Published: Jan. 1, 2025

Language: Английский

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An Antibacterial, Antioxidant Adherent Sponge Constructed for Control of Arterial Bleeding Via Gallic Acid-Mediated Robust Assembly of Fibrous Clay in Collagen

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Acute hemorrhage death on battlefields, during clinical surgeries, and in major accidents is a widespread worldwide problem. Clay-based hemostatic materials have received considerable attention for their low cost reliable clotting activity, especially cases of severe bleeding, such as QuikClot, which kaolin-based gauze that preferred battlefield resuscitation. However, the easy detachment clay particles associated risk thrombosis seriously hindered development clay-based materials. Here, inexpensive palygorskite (Pal) nanoclay was integrated into collagen (COL) matrix by loading Ca2+ further using gallic acid (GA) to mediate robust assembly COL matrix. This targeted interfacial design simple gentle method effectively improves dispersion Pal reduces shedding. Unlike QuikClot where aqueous solution significantly turbid after 2 min ultrasonic washing, composite sponge (Ca-Pal-GA-COL) remained clear accompanied 82.71% mass residue 10 washing. The also exhibited excellent antibacterial (87.93% inhibition rate Escherichia coli), antioxidant, tissue adhesion properties. Importantly, Ca-Pal-GA-COL less blood loss (632 mg) shorter hemostasis time (151 s) rat femoral artery model than medical (3850 mg 299 s), pure (1627 201 Pal-COL (1494 193 co-mingled mode, are comparable those (559 142 s). Furthermore, certain properties render more suitable active bleeding scenarios. Cellular experiments confirmed dressing has biosafety.

Language: Английский

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Silk fibroin methacryloyl hydrogel loaded with silver-gallic acid nanoparticles for enhanced diabetic wound healing

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142108 - 142108

Published: March 1, 2025

Language: Английский

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Post-Stroke Recovery: A Review of Hydrogel-Based Phytochemical Delivery Systems

Gels, Journal Year: 2025, Volume and Issue: 11(4), P. 260 - 260

Published: April 1, 2025

Stroke remains a leading cause of disability worldwide, underscoring the urgent need for novel and innovative therapeutic strategies to enhance neuroprotection, support regeneration, improve functional recovery. Previous research has shown that phytochemicals such as curcumin, tannic acid, gallic ginsenosides, resveratrol, isorhamnetin display extensive neuroprotective properties, including antioxidant, anti-inflammatory, anti-apoptotic effects. These natural compounds could also promote neurogenesis, angiogenesis, preservation blood–brain barrier. Despite their promising bioactivities, clinical application is often limited by poor solubility, bioavailability, suboptimal pharmacokinetics. Hydrogels offer solution encapsulating controlling gradual release these directly at site injury. Recent advancements in hydrogel formulations, constructed from biopolymers functionalized using nanotechnological approaches, significantly stability, targeted delivery phytochemicals. Controlled profiles pH-sensitive environment-responsive hydrogels ensure compounds’ effects are optimally timed with individual critical stages post-stroke repair. Moreover, scaffolds tailored material properties biocompatibility can create favorable microenvironment, reducing secondary inflammation, enhancing tissue potentially improving cognitive outcomes following stroke. This review explores potential integrating within hydrogel-based systems specifically designed The design synthesis biocompatible, biodegradable especially applications discussed. Lastly, we emphasize additional robust translatable preclinical studies.

Language: Английский

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Bone Morphogenetic Protein-2–Derived Peptide-Conjugated Nanozyme-Integrated Photoenhanced Hybrid Hydrogel for Cascade-Regulated Bone Regeneration

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Critical-sized bone defects present a clinical challenge due to their limited self-repair capacity. Application of tissue-engineering scaffolds often overlooks the dynamic modulation microenvironment, resulting in unsatisfactory bone-regeneration outcomes. In this study, morphogenetic protein-2-derived peptide-loaded honeycomb manganese dioxide (BHM) nanozyme was incorporated into composite hydrogel (BHM@CG) composed l-arginine-modified methacrylated carboxymethyl chitosan and gallic acid-grafted gelatin. This demonstrated cascade-regulated enhancement hemostasis, antibacterial activity, anti-inflammatory effects, osteogenesis. Initially, BHM@CG achieved rapid hemostasis by quickly adhering irregular upon injury. Subsequently, it displayed robust activity through synergistic hydrogen bonding, hydrophobic interactions, cationic interactions. Meanwhile, BHM polyphenol groups from acid effectively eliminated reactive oxygen species, enabling long-term inflammation regulation. Finally, sustained release bioactive components promoted cell migration, angiogenesis, osteogenesis, achieving bone-formation rate nearly 40% critical-sized calvarial defect model week 8. More interestingly, also efficient capabilities an infected model. Overall, dynamically modulated bone-defect microenvironment enhanced regeneration, offering promising strategy for repair.

Language: Английский

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Antioxidant and Anti‐Senescence Polyvinyl Alcohol‐Gallic Acid Supramolecular Hydrogels for Stem Cell Culture

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Abstract Replicative senescence presents a significant challenge in mesenchymal stem cell (MSC) expansion due to high reactive oxygen species (ROS) levels generated during culture. Elevated ROS lead oxidative stress, cellular damage, and senescence, limiting the biomedical applications of MSCs. In this study, supramolecular thermo‐reversible hydrogel composed natural polyphenolic compound gallic acid (GA) polyvinyl alcohol (PVA) was designed scavenge mitigate MSC senescence. The PVA‐GA hydrogel, stabilized by strong hydrogen bonding forces, exhibited an elastic modulus comparable that human soft tissue facilitated sustained release GA over 14 days. It enhanced survival, protected against reduced intracellular levels, diminished mitochondrial decreased maintained multilineage differentiation potential typical phenotype Additionally, it preserved vascular endothelial growth factor (VEGF) secretion from MSCs under stress their pro‐angiogenic effect. conditioned medium derived group promoted migration tube formation umbilical vein cells (HUVECs). These findings suggest holds promise for MSCs, potentially addressing challenges posed

Language: Английский

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Chitosan-Gallic Acid Conjugate with Enhanced Functional Properties and Synergistic Wound Healing Effect

Carbohydrate Research, Journal Year: 2025, Volume and Issue: unknown, P. 109496 - 109496

Published: April 1, 2025

Language: Английский

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Light-Mediated 3D-Printed Wound Dressings Based on Natural Polymers with Improved Adhesion and Antioxidant Properties

Polymers, Journal Year: 2025, Volume and Issue: 17(8), P. 1114 - 1114

Published: April 20, 2025

The lack of personalized wound dressings tailored to individual needs can significantly hinder healing. Hydrogels offer a promising solution, as they be engineered mimic the extracellular matrix (ECM), providing an optimal environment for repair. integration digital light processing (DLP), high-resolution 3D printing process, allows precise customization hydrogel-based dressings. In this study, gelatin methacrylate (GelMA)-based formulations were prepared in combination with three different polymeric precursors: methacrylated hyaluronic acid (HAMA), poly (ethylene glycol) diacrylate (PEGDA) and allyl cellulose (MCCA). These precursors used print micropatterned patches. printed constructs revealed high gel content good resistance hydrolytic degradation. To improve adhesive antioxidant properties patches, gallic (GA) was incorporated through surface functionalization. This enabled scavenging approximately 80% free radicals within just 4 h. also improved, further enhancement observed upon addition Fe3+ ions. vitro cytocompatibility tests using fibroblast (NHDF) cell line confirmed suitability materials biomedical applications. Thus, study demonstrates potential DLP-printed hydrogels advanced dressing materials.

Language: Английский

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Gallic acid regulates primary root elongation via modulating auxin transport and signal transduction

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 2, 2024

Gallic acid is an important secondary metabolite in plants, with great value medicine, food, and chemical industry. However, whether how this widely existing natural polyphenolic compound affects the growth development of plants themselves remains elusive. In study, we revealed that exogenous application gallic has a dual effect on elongation primary root Arabidopsis. While lower concentrations slightly stimulate growth, excessive profoundly reduces length meristem size dose-dependent manner, probably via suppressing cell division as indicated by CYCB1;1::GUS . Moreover, suggested DR5::GFP line analysis confirmed LC-MS assay, auxin contents tips were dramatically decreased upon treatment. Additional IAA partially rescued shortened treatment, suggesting required for acid-caused inhibition. Then, further down-regulated expression transporters PIN1 , PIN2 PIN3 PIN7 triple mutant pin1 pin3 pin7 exhibited reduced sensitivity to Meanwhile, degradation AXR3/IAA17 protein HS::AXR3NT-GUS reporter line. Auxin signaling tir1 afb2 afb3 axr3-3 also less sensitive treatment terms size. Taken together, these findings inhibits modulating transport

Language: Английский

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