Dalton Transactions, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 28, 2024
Schematic diagram of the Ce-doped MOF nanozymes that mimic peroxidase enzyme. The corresponding mechanistic cycle is also shown.
Language: Английский
ChemElectroChem, Journal Year: 2024, Volume and Issue: 11(11)
Published: April 8, 2024
Abstract Chirality plays a significant role in the manufacture of pharmaceuticals and fine chemicals. The use chemical catalysts to control stereoselectivity relies on chiral with labor–intensive synthesis purification. Natural enzymes offer inherent stereoselectivity, making them attractive for this purpose. We report here biocatalytic oxidations microemulsions driven by horseradish peroxidase coupled synthetic Cu 2+ ‐polymer catalyst. This hybrid system features crosslinked layer–by–layer (LBL) films composed polyions ‐containing pyrene–labelled poly(2‐hydroxy‐3‐dipicolylamino) propyl methacrylate (Py−PGMADPA) drive oxygen reduction form hydrogen peroxide. Peroxide turn activates (HRP) LbL magnetic particle beads biocatalytically oxidize styrene, ethylbenzene, methyl phenylacetate products. R‐stereoisomers these reactants were selectively formed high enantiomeric excess ≥80 % at 90 °C. enzyme show thermal stability °C cetyltrimethylammonium bromide microemulsion. Reactions essentially complete 2 hr. approach opens door new designs syntheses using separate electrocatalyst activation.
Language: Английский
Citations
1
ChemCatChem, Journal Year: 2024, Volume and Issue: 16(13)
Published: May 9, 2024
Abstract In chemical industrial process, conversion of alkenes to functional oxygenates plays a key role in bridging between the feedstock and downstream application. Traditionally, oxidation required highly‐active oxygen sources or molecular under high temperature pressure. This poses challenges green safe production oxygenates. To circumvent issues massive consumption fossil fuel serious CO 2 footprints, approaches electro‐oxidation are developed ambient conditions, adopting O H as source. Utilizing electricity from renewable resources involved electron transfer, performance is closely related interface characteristics electrodes electrolytes. review presents progress catalysts design, electrolytes optimization, cells integration effectively regulate microenvironment interfaces. Further discussion mechanism analysis potential emphasized, based on in‐situ characterization techniques, isotopic experiments quenching tests. We hope this can provide an outline for via electrocatalytic pathways.
Language: Английский
Citations
1
Metabolites, Journal Year: 2024, Volume and Issue: 14(8), P. 429 - 429
Published: Aug. 3, 2024
Human liver subcellular fractions, including microsomes (HLM), cytosol and S9 are extensively utilized in vitro assays to predict metabolism. The fractions supernatants of human homogenates that contain both cytosol, which include most cytochrome P450 (CYP) enzymes soluble phase II such as glucuronosyltransferases sulfotransferases. This study reports on the direct electrochemistry biocatalytic features redox-active for first time. We investigated electrochemical properties films by immobilizing them onto a high-purity graphite (HPG) electrode performing cyclic voltammetry under anaerobic (Ar-saturated) aerobic (O2-saturated) conditions. heterogeneous electron transfer rate between film HPG was found be 14 ± 3 s-1, with formal potential -0.451 V vs. Ag/AgCl reference electrode, confirmed activation FAD/FMN cofactor containing CYP450-reductase (CPR) receiver from electrode. have also demonstrated catalytic oxygen reduction conditions, identical HLM attached similar electrodes. Additionally, we CYP activity biofilm I metabolism using diclofenac hydroxylation probe reaction identified metabolic products liquid chromatography-mass spectrometry (LC-MS). Investigating feasibility utilizing offers significant advantages pharmacological toxicological evaluations new drugs development while providing valuable insights efficient biosensor bioreactor platforms.
Language: Английский
Citations
1
Biosensors, Journal Year: 2023, Volume and Issue: 13(9), P. 897 - 897
Published: Sept. 21, 2023
Breast cancer (BC) is one of the most common types disease worldwide and it accounts for thousands deaths annually. Lapatinib among preferred drugs treatment breast cancer. Possible drug toxicity effects lapatinib can be controlled by real-time determination appropriate dose a patient at point care. In this study, novel highly sensitive polymeric nanobiosensor presented. A composite poly(anilino-co-4-aminobenzoic acid) co-polymer {poly(ANI-co-4-ABA)} coffee extract-based green-synthesized indium nanoparticles (InNPs) was used to develop sensor platform on screen-printed carbon electrode (SPCE), i.e., SPCE||poly(ANI-co-4-ABA-InNPs). Cytochrome P450-3A4 (CYP3A4) enzyme polyethylene glycol (PEG) were incorporated modified produce SPCE||poly(ANI-co-4-ABA-InNPs)|CYP3A4|PEG nanobiosensor. Experiments electrochemical response characteristics performed with cyclic voltammetry (CV) differential pulse (DPV). The calibration 0–100 ng/mL linear gave limit detection (LOD) values 13.21 18.6 in physiological buffer human serum, respectively. LOD are much lower than peak plasma concentration (Cmax) (2.43 µg/mL), which attained 4 h after administration daily 1250 mg lapatinib. also exhibited excellent anti-interference performance stability.
Language: Английский
Citations
3
Published: Jan. 1, 2024
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
Language: Английский
Citations
0
BioNanoScience, Journal Year: 2024, Volume and Issue: 14(3), P. 2930 - 2939
Published: July 12, 2024
Language: Английский
Citations
0
Biomedical Chemistry Research and Methods, Journal Year: 2024, Volume and Issue: 7(3), P. e00225 - e00225
Published: Jan. 1, 2024
The review is devoted to new highly effective methods for analyzing the catalytic activity of enzymes medical significance, such as cytochromes P450, trypsin, asparaginase, beta-lactamase, and nucleases. are based on registration specific using electroanalytical methods. analyzes experimental data obtained by authors. Two platforms have been developed that allow quantitative measurement electrochemical properties enzyme (cytochrome bactosomes, asparaginase) or substrate (trypsin, nucleases, restriction enzymes, beta-lactamase).
Language: Английский
Citations
0
ACS Catalysis, Journal Year: 2024, Volume and Issue: 14(21), P. 16344 - 16352
Published: Oct. 22, 2024
We herein report a design of artificial enzymes by incorporating synthetic copper complex into noncatalytic bovine serum albumin (Cu-BSA) to carry out stereoselective oxidation. This Cu-BSA catalyst with stably bound Cu as cofactor shows peroxidase-like activity catalyze epoxidation styrene high chiral selectivity (>99%) R-styrene epoxide. With the electrochemical conversion Cu2+ Cu+, also exhibits oxidase-like selectively reduce oxygen hydrogen peroxide (H2O2), which can be combined its peroxidase function drive oxidation C═C bonds using air. enzymatic system holds promise for chiral-selective transformations non-natural substances and highlights versatility proteins in enzymes.
Language: Английский
Citations
0
Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136476 - 136476
Published: Nov. 12, 2024
Language: Английский
Citations
0
Dalton Transactions, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 28, 2024
Schematic diagram of the Ce-doped MOF nanozymes that mimic peroxidase enzyme. The corresponding mechanistic cycle is also shown.
Language: Английский
Citations
0