Extracellular Vesicles Derived from Selenium-Deficient MAC-T Cells Aggravated Inflammation and Apoptosis by Triggering the Endoplasmic Reticulum (ER) Stress/PI3K-AKT-mTOR Pathway in Bovine Mammary Epithelial Cells

Antioxidants, Journal Year: 2023, Volume and Issue: 12(12), P. 2077 - 2077

Published: Dec. 5, 2023

Selenium (Se) deficiency disrupts intracellular REDOX homeostasis and severely deteriorates immune anti-inflammatory function in high-yielding periparturient dairy cattle. To investigate the damage of extracellular vesicles derived from Se-deficient MAC-T cells (SeD-EV) on normal mammary epithelial cells, an vitro model Se was established. produced many ROS, promoting apoptosis release inflammatory factors. Extracellular were successfully isolated by ultrahigh-speed centrifugation identified transmission electron microscopy, particle size analysis, surface markers (CD63, CD81, HSP70, TSG101). RNA sequencing performed exosomal RNA. A total 9393 lncRNAs 63,155 mRNAs transcripts SeC SeD groups, respectively, which 126 955 differentially expressed. Furthermore, SeD-EV promoted TUNEL analysis. significantly inhibited Bcl-2, while Bax Cleaved Caspase3 greatly increased. Antioxidant capacity (CAT, T-AOC, SOD, GSH-Px) SeD-EV-treated cells. Additionally, p-PERK, p-eIF2α, ATF4, CHOP, XBP1 all elevated supplemented with SeD-EV. In addition, p-PI3K, p-Akt, p-mTOR decreased strikingly conclusion, caused oxidative stress, thus triggering inflammation through endoplasmic reticulum stress PI3K-Akt-mTOR signaling pathway, contributed to explaining mechanism causing mastitis.

Language: Английский

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Advances of Selenium in Poultry Nutrition and Health

Journal of Nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Eleutheroside B Ameliorates Cardiomyocytes Necroptosis in High-Altitude-Induced Myocardial Injury via Nrf2/HO-1 Signaling Pathway

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 190 - 190

Published: Feb. 7, 2025

This study was designed to evaluate the protective effects of eleutheroside B (EB) in high-altitude-induced myocardial injury (HAMI) and unravel underlying molecular mechanisms. SD rats were used for vivo experiments. Following pretreatment with EB, exposed a hypobaric environment within chamber 48 h. Electrocardiograms, H&E staining, serum biochemical indices measured EB on HAMI. Immunofluorescence Western blotting utilized detect expression associated proteins. In parallel, hypoxic cell incubator establish an vitro model hypoxia-induced injury. The anti-necroptotic effect its potential mechanisms investigated verified vitro. Exposure hypoxia led electrocardiogram disorders, pathological changes tissue, increased concentrations BNP CK-MB, elevated levels oxidative stress indicators inflammatory factors. Additionally, necroptosis-related proteins upregulated. Pretreatment effectively ameliorated caused by hypoxia, mitigated inflammation, suppressed necroptosis. Furthermore, facilitated translocation Nrf2 into nucleus. conclusion, this provides evidence suggesting that may exert against HAMI inhibiting cardiomyocyte necroptosis via Nrf2/HO-1 signaling pathway.

Language: Английский

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Cytomorphological assessment of the effect of selenium nanoparticles on SPEV-2 oncovirus cells ‎

Journal Of Advanced Pharmacy Education And Research, Journal Year: 2024, Volume and Issue: 14(1), P. 84 - 90

Published: Jan. 1, 2024

Language: Английский

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