Oral Administration of Lactobacillus gasseri and Lacticaseibacillus rhamnosus Ameliorates Amyloid Beta (Aβ)-Induced Cognitive Impairment by Improving Synaptic Function Through Regulation of TLR4/Akt Pathway

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 139 - 139

Published: Jan. 24, 2025

This study investigated the anti-amnesic effects of Lactobacillus gasseri (L. gasseri) MG4247 and Lacticaseibacillus rhamnosus rhamnosus) MG4644 in amyloid beta (Aβ)-induced mice. We confirmed that oral administration L. ameliorated cognitive impairment Aβ-induced mice using Y-maze, passive avoidance, Morris water maze tests. Oral protected antioxidant system by regulating superoxide dismutase levels, reduced glutathione malondialdehyde contents. Similarly, they attenuated mitochondrial function decreasing reactive oxygen species levels increasing membrane potential ATP levels. In addition, regulated neuroinflammation neurotoxicity modulating Toll-like receptor 4 (TLR4)/protein kinase B (Akt) pathway. As a result, enhanced synaptic acetylcholine contents, acetylcholinesterase activity, expression synaptic-function-related proteins such as AChE, ChAT, SYP, PSD-95, GAP-43. Furthermore, improved dysbiosis promoting growth beneficial bacteria while suppressing harmful bacteria. Therefore, these results suggest may be used probiotics to prevent impairment.

Language: Английский

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Lycium ruthenicum Murray exosome-like nanovesicles alleviated Alzheimer's disease–like symptoms induced by Aβ protein in transgenic Caenorhabditis elegans through the DAF-16 pathway

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 304, P. 140758 - 140758

Published: Feb. 6, 2025

Language: Английский

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Gut-Brain Axis-Based Polygala Tenuifolia and Magnolia Officinalis Improve D-gal-Induced Cognitive Impairment in Mice Through cAMP and NF-κB Signaling Pathways

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 1869 - 1894

Published: March 1, 2025

Polygala tenuifolia Willd. (PT) is commonly used to address cognitive impairment (CI), while Magnolia officinalis Rehd. et Wils (MO) often prescribed for gastrointestinal issues as well CI. This study seeks explore the impacts and mechanisms behind combined therapy of PT MO (PM) in treating CI, based on concept gut-brain axis. The characteristic components PT, MO, PM were identified using ultra-high performance liquid chromatography-tandem triple quadrupole mass Spectrometry (UPLC-MS/MS). A mouse model was established by D-gal induction, effects CI evaluated through behavioral tests, pathological staining, Enzyme-Linked Immunosorbent Assay (ELISA). Subsequently, network pharmacology analyze potential which improves followed validation Western blotting (WB), traditional Chinese medicine (TEM), Immunofluorescence (IF), 16S rRNA. can each alleviate decline neuropathological damage mice varying degrees, reduce expression pro-inflammatory factors (TNF-α, IL-1β, IL-6, IFN-γ, LPS) serum or hippocampal tissue, increase SOD GSH levels. Network analysis molecular experiments confirmed that upregulates tight junction s (TJs), enhances proteins cAMP pathway, inhibits p-NF-κB-p65 expression. reverses D-gal-induced gut microbiota dysbiosis, increases abundance SCFA-producing bacteria, decreases LPS-producing bacteria. alleviates reducing inflammation oxidative stress, protecting blood-brain barrier (BBB) intestinal barrier, inhibiting NF-κB activating regulating microbiota.

Language: Английский

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Emulsion-Enhanced Synergy of Walnut-Derived Hexapeptide (EPEVLR) and DHA in Mitigating D-Galactose-Induced Cognitive Decline in Mice

Food Hydrocolloids for Health, Journal Year: 2025, Volume and Issue: unknown, P. 100215 - 100215

Published: April 1, 2025

Language: Английский

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