LPL-RH suppresses bone loss in ovariectomised rat models DOI Creative Commons
Wen‐jie Chen,

Xinliang Wang,

Yufan Wang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Evidence has revealed that oestrogen deprivation-induced osteolysis is microbiota-dependent and can be treated by probiotics. However, the underlying mechanism require further investigation. This study aims to provide additional evidence supporting use of probiotics as an adjuvant treatment explore pathophysiology oestrogen-deprived osteolysis. Forty-five SD rats were randomly divided into five groups (n = 9). Rats from four ovariectomised with NS, calcium, probiotics, or calcium + while one group underwent a sham operation was NS. The osteometabolic effects evaluated, mechanistic role probiotic supplement explored. Intragastric administration Bifidobacterium animalis subsp. lactis LPL-RH (LPL-RH) markedly suppressed osteoclastic activation bone loss downregulating TRAP enzymatic activity, OPG/RANKL ratio, downstream signalling pathway RANKL/TRAF6/NF-κB/NFATc1/TRAP in rats. also reduced CD4+IL-17 A+ TH17 cells marrow, pro-osteoclastogenic cytokine IL-17 A, pro-inflammatory molecules (LPS), its binding protein (LBP) blood. restored intestinal ZO-1, occludin, claudin 2, 12, 15, which improved ileal histopathology, oxidative stress, attenuated LPS-responsive TLR4/MyD88/NF-κB pathway. Furthermore, 16 S rRNA sequencing altered faecal microbiome reducing relative abundance S24-7 at family level promoting Prevotella Bacteroides genus level. Collectively, osteoclastogenesis modulating type 17 immunity gut microbiome.

Language: Английский

LPL-RH suppresses bone loss in ovariectomised rat models DOI Creative Commons
Wen‐jie Chen,

Xinliang Wang,

Yufan Wang

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 28, 2024

Evidence has revealed that oestrogen deprivation-induced osteolysis is microbiota-dependent and can be treated by probiotics. However, the underlying mechanism require further investigation. This study aims to provide additional evidence supporting use of probiotics as an adjuvant treatment explore pathophysiology oestrogen-deprived osteolysis. Forty-five SD rats were randomly divided into five groups (n = 9). Rats from four ovariectomised with NS, calcium, probiotics, or calcium + while one group underwent a sham operation was NS. The osteometabolic effects evaluated, mechanistic role probiotic supplement explored. Intragastric administration Bifidobacterium animalis subsp. lactis LPL-RH (LPL-RH) markedly suppressed osteoclastic activation bone loss downregulating TRAP enzymatic activity, OPG/RANKL ratio, downstream signalling pathway RANKL/TRAF6/NF-κB/NFATc1/TRAP in rats. also reduced CD4+IL-17 A+ TH17 cells marrow, pro-osteoclastogenic cytokine IL-17 A, pro-inflammatory molecules (LPS), its binding protein (LBP) blood. restored intestinal ZO-1, occludin, claudin 2, 12, 15, which improved ileal histopathology, oxidative stress, attenuated LPS-responsive TLR4/MyD88/NF-κB pathway. Furthermore, 16 S rRNA sequencing altered faecal microbiome reducing relative abundance S24-7 at family level promoting Prevotella Bacteroides genus level. Collectively, osteoclastogenesis modulating type 17 immunity gut microbiome.

Language: Английский

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