Microbial electrosynthesis: opportunities for microbial pure cultures

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(8), P. 1035 - 1047

Published: March 1, 2024

Microbial electrosynthesis (MES) is an emerging technology that couples renewable electricity to microbial production processes. Although advances in MES performance have been driven largely by mixed cultures, we see a great limitation the diversity, and hence value, of products can be achieved undefined cultures. By contrast, metabolic control pure cultures genetic engineering could greatly expand scope MES, even broader electrobiotechnology, include targeted high-value products. To leverage this potential, advocate for more efforts activities develop engineered electroactive microbes synthesis, highlight need standardized electrobioreactor infrastructure allows establishment electrobioprocesses with these novel biocatalysts.

Language: Английский

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Electrobiocorrosion by microbes without outer‐surface cytochromes

mLife, Journal Year: 2024, Volume and Issue: 3(1), P. 110 - 118

Published: March 1, 2024

Abstract Anaerobic microbial corrosion of iron‐containing metals causes extensive economic damage. Some microbes are capable direct metal‐to‐microbe electron transfer (electrobiocorrosion), but the prevalence electrobiocorrosion among diverse methanogens and acetogens is poorly understood because a lack tools for their genetic manipulation. Previous studies have suggested that respiration with 316L stainless steel as donor indicative electrobiocorrosion, because, unlike pure Fe 0 , does not abiotically generate H 2 an intermediary carrier. Here, we report all ( Methanosarcina vacuolata, Methanothrix soehngenii Methanobacterium strain IM1) Sporomusa ovata Clostridium ljungdahlii ) evaluated respired donor, only M. Mx. S. were electrobiocorrosion. The electrobiocorrosive required acetate additional energy source in order to produce methane from steel. Cocultures demonstrated how can provide during corrosion. Not was IM1 it also did accept electrons Geobacter metallireducens effective electron‐donating partner interspecies directly . finding despite outer‐surface c ‐type cytochromes previously found be important other microbes, demonstrates there multiple strategies making electrical contact

Language: Английский

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Electrical-energy storage into chemical-energy carriers by combining or integrating electrochemistry and biology

Energy & Environmental Science, Journal Year: 2024, Volume and Issue: 17(11), P. 3682 - 3699

Published: Jan. 1, 2024

Our societies must reconsider current industrial practices and find carbon-neutral alternatives to avoid the detrimental environmental effects that come with release of greenhouse gases from fossil-energy carriers.

Language: Английский

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Advanced Electroanalysis for Electrosynthesis

ACS Organic & Inorganic Au, Journal Year: 2023, Volume and Issue: 4(2), P. 141 - 187

Published: Nov. 29, 2023

Electrosynthesis is a popular, environmentally friendly substitute for conventional organic methods. It involves using charge transfer to stimulate chemical reactions through the application of potential or current between two electrodes. In addition electrode materials and type reactor employed, strategies controlling have an impact on yields, product distribution, reaction mechanism. this Review, recent advances related electroanalysis applied in electrosynthesis were discussed. The first part study acts as guide that emphasizes foundations electrosynthesis. These essentials include instrumentation, selection, cell design, methodologies. Then, electroanalytical techniques organic, enzymatic, microbial are illustrated with specific cases studied literature. To conclude, discussion future possibilities intend advance academic industrial areas presented.

Language: Английский

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Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: July 11, 2024

Acetogenic bacteria (acetogens) are a class of microorganisms with conserved Wood-Ljungdahl pathway that can utilize CO and 2 /H as carbon source for autotrophic growth convert these substrates to acetate ethanol. Acetogens have great potential the sustainable production biofuels bulk biochemicals using C1 gases (CO ) from industrial syngas waste gases, which play an important role in achieving neutrality. In recent years, development improvement gene editing methods, metabolic engineering acetogens is making rapid progress. With introduction heterogeneous pathways, improve capacity native products or obtain ability synthesize non-native products. This paper reviews application acetogens. addition, challenges indicated, strategies address also discussed.

Language: Английский

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Integration of biocompatible hydrogen evolution catalyst developed from metal-mix solutions with microbial electrosynthesis

Bioelectrochemistry, Journal Year: 2024, Volume and Issue: 158, P. 108724 - 108724

Published: May 3, 2024

Microbial conversion of CO

Language: Английский

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Effect of different hydrogen evolution rates at cathode on bioelectrochemical reduction of CO2 to acetate

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 913, P. 169744 - 169744

Published: Jan. 3, 2024

Language: Английский

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Nonelectroactive clostridium obtains extracellular electron transfer-capability after forming chimera with Geobacter

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Extracellular electron transfer (EET) of microorganisms is a major driver the microbial growth and metabolism, including reactions involved in cycling C, N, Fe anaerobic environments such as soils sediments. Understanding mechanisms EET, well knowing which organisms are EET-capable (or can become so) fundamental to electromicrobiology geomicrobiology. In general, Gram-positive bacteria very seldomly perform EET due their thick non-conductive cell wall. Here, we report that Clostridium intestinale (C.i) attained EET-capability for ethanol metabolism only after forming chimera with electroactive Geobacter sulfurreducens (G.s). Mechanism analyses demonstrated was possible fusion two species achieved. Under these conditions, pathway C.i integrated by G.s, achieved oxidation subsequent reduction extracellular acceptors coculture. Our study displays new approach via recruiting an bacterium, suggests previously unanticipated prevalence world. These findings also provide perspectives understand energetic coupling between bacterial ecology interspecies mutualisms.

Language: Английский

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Mechanism, performance enhancement, and economic feasibility of CO2 microbial electrosynthesis systems: A data-driven analysis of research topics and trends

Renewable and Sustainable Energy Reviews, Journal Year: 2024, Volume and Issue: 202, P. 114704 - 114704

Published: June 28, 2024

Language: Английский

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Advanced aspects of acetogens

Bioresource Technology, Journal Year: 2024, Volume and Issue: unknown, P. 131913 - 131913

Published: Dec. 1, 2024

Language: Английский

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