Organic & Biomolecular Chemistry,
Journal Year:
2024,
Volume and Issue:
22(19), P. 3876 - 3881
Published: Jan. 1, 2024
Predicting
selectivity
and
conversion
in
a
confined
reaction
medium
under
photochemical
conditions
is
highly
challenging
as
compared
to
the
corresponding
conventional
synthesis.
Herein,
we
report
use
of
simple
carbohydrate-derived
eutectogel
facilitate
LED-light-induced
regioselective
synthesis
4-arylamino-1,2-naphthoquinones
good
yield.
This
methodology,
by
including
reusable
medium,
proved
have
potential
affording
formation
various
desired
products
yields.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(2), P. 284 - 284
Published: Jan. 13, 2025
Essential
oils
(EOs)
are
highly
valued
in
the
cosmetic
and
food
industries
for
their
diverse
properties.
However,
traditional
extraction
methods
often
result
low
yields,
inconsistent
compositions,
lengthy
times,
use
of
potentially
harmful
solvents.
Natural
deep
eutectic
solvents
(NADES)
have
emerged
as
promising
alternatives,
offering
advantages
such
higher
efficiency,
cost-effectiveness,
biodegradability,
tunable
This
review
explores
application
NADES
enhancing
EO
extraction,
focusing
on
current
methodologies,
key
insights,
practical
applications.
It
examines
factors
that
influence
with
NADES,
including
optimization
physicochemical
properties,
techniques,
operational
conditions,
role
sample
pretreatment
improving
efficiency.
Additionally,
this
covers
chemical
characterization
biological
activities
EOs
extracted
using
NADES.
By
providing
a
comprehensive
overview,
it
highlights
potential
to
improve
suggests
directions
future
research
field.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 29, 2025
Summary
Establishment
of
N.
benthamiana
as
a
robust
biofactory
is
complicated
by
issues
such
product
toxicity
and
proteolytic
degradation
target
proteins
/
introduced
enzymes.
Here
we
investigate
whether
biomolecular
condensates
can
be
used
to
address
these
problems.
We
engineered
in
leaves
using
transient
expression
synthetic
modular
scaffolds.
The
vivo
properties
the
that
resulted
were
consistent
with
them
being
liquid-like
bodies
thermodynamic
features
typical
multicomponent
phase-separating
systems.
show
recruitment
enzymes
led
several
fold
yield
increases
one-
three-step
metabolic
pathways
(citramalate
biosynthesis
poly-3-hydroxybutyrate
(PHB)
biosynthesis,
respectively).
This
enhanced
could
for
reasons
including
improved
enzyme
kinetics,
metabolite
channelling
or
avoidance
cytotoxicity
retention
pathway
within
condensate,
which
was
demonstrated
PHB.
However,
also
observed
several-fold
increase
amount
accumulated
when
they
targeted
condensates.
suggests
more
stable
localised
condensate
than
freely
diffusing
cytosol.
hypothesise
this
stability
likely
main
driver
increased
production.
Our
findings
provide
foundation
leveraging
plant
engineering
advance
versatile
industrial
applications.
Plant Biotechnology Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 9, 2025
Summary
The
establishment
of
Nicotiana
benthamiana
as
a
robust
biofactory
is
complicated
by
issues
such
product
toxicity
and
proteolytic
degradation
target
proteins/introduced
enzymes.
Here
we
investigate
whether
biomolecular
condensates
can
be
used
to
address
these
problems.
We
engineered
in
N.
leaves
using
transient
expression
synthetic
modular
scaffolds.
vivo
properties
the
that
resulted
were
consistent
with
them
being
liquid‐like
bodies
thermodynamic
features
typical
multicomponent
phase‐separating
systems.
show
recruitment
enzymes
led
several‐fold
yield
increases
one‐
three‐step
metabolic
pathways
(citramalate
biosynthesis
poly‐3‐hydroxybutyrate
(PHB)
biosynthesis,
respectively).
This
enhanced
could
for
several
reasons
including
improved
enzyme
kinetics,
metabolite
channelling
or
avoidance
cytotoxicity
retention
pathway
within
condensate,
which
was
demonstrated
PHB.
However,
also
observed
increase
amount
accumulated
when
they
targeted
condensates.
suggests
more
stable
localised
condensate
than
freely
diffusing
cytosol.
hypothesise
this
stability
likely
main
driver
increased
production.
Our
findings
provide
foundation
leveraging
plant
engineering
advance
versatile
industrial
applications.
Separation Science Plus,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 1, 2024
ABSTRACT
Piper
betle
L.
(Piperaceae)
is
a
herbal
plant
used
in
Thai
traditional
medicine
for
its
antifungal
and
antiallergic
properties,
particularly
through
topical
application.
This
study
aimed
to
optimize
the
extraction
of
leaves
using
natural
deep
eutectic
solvents
enhance
efficiency
eugenol,
active
compound
leaves.
Various
types
hydrogen
bond
donors—including
glycolic
acid,
lactic
malic
tartaric
ascorbic
citric
acid—were
combined
with
acceptor,
choline
chloride,
prepare
solvents.
The
results
indicated
that
acid
was
most
effective
donor
eugenol
extraction.
Subsequently,
chloride:lactic
molar
ratios,
solvent:water
mass
ultrasonication
times
were
varied.
Results
showed
optimal
conditions
based
on
one‐factor‐at‐a‐time
approach
ratio
1:5,
2:1,
an
time
30
min.
Under
these
conditions,
concentration
reached
457.22
±
21.68
µg/mL,
compared
224.76
6.99
µg/mL
when
ethanol
used.
In
conclusion,
this
successfully
optimized
from
These
findings
suggest
could
be
more
solvent
eugenol.
