Unravelling the stabilization mechanism of mono-, di and tri-cholinium citrate-ethylene glycol DESs towards α-chymotrypsin for preservation and activation of the enzyme DOI

Bindu Yadav,

Niketa Yadav,

Pannuru Venkatesu

et al.

Physical Chemistry Chemical Physics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The present study demonstrates the biocompatibility of new green solvents to achieve increased stability, activity and prolonged storage α-CT.

Language: Английский

Novel alcohol dehydrogenase activation by the choline component of Deep eutectic solvents DOI
Ebin K. Baby, Rangasamy Savitha, Gemma K. Kinsella

et al.

Journal of Molecular Liquids, Journal Year: 2025, Volume and Issue: unknown, P. 127025 - 127025

Published: Jan. 1, 2025

Language: Английский

Citations

1
Solvent Tolerance Improvement of Lipases Enhanced Their Applications: State of the Art DOI Creative Commons

Mei Chen,

Tongtong Jin,

Binbin Nian

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2444 - 2444

Published: May 22, 2024

Lipases, crucial catalysts in biochemical synthesis, find extensive applications across industries such as food, medicine, and cosmetics. The efficiency of lipase-catalyzed reactions is significantly influenced by the choice solvents. Polar organic solvents often result a decrease, or even loss, lipase activity. Conversely, nonpolar induce excessive rigidity lipases, thereby affecting their While advent new like ionic liquids deep eutectic has somewhat improved activity stability it fails to address fundamental issue lipases' poor solvent tolerance. Hence, rational design lipases for enhanced tolerance can boost industrial performance. This review provides comprehensive summary structural characteristics properties various systems emphasizes strategies protein engineering non-aqueous media improve study theoretical foundation further enhancing lipases.

Language: Английский

Citations

7
Computer-assisted enzyme cocktails enhance fermentation by overcoming toxic inhibitors from pretreatment processes DOI
Minghui Wang,

Yibo Song,

Hu Meng

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: 419, P. 132076 - 132076

Published: Jan. 18, 2025

Language: Английский

Citations

0
Recent advancements in Deep Eutectic Solvent (DES) pretreatment: Applications, mechanisms, and integration with emerging technologies for biorefinery DOI Creative Commons
Minghui Wang,

Xianshen Fu,

Yu‐Ping Chang

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 229, P. 121028 - 121028

Published: April 22, 2025

Language: Английский

Citations

0
Optimization of Lipase Activity in Aspergillus niger C2J6 Whole Cells Using Choline Chloride Ethylene Glycol Deep Eutectic Solvent DOI

Qingxiu Ma,

MuGuo Tang,

Yinsong Wan

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Abstract Deep eutectic solvents (DES), recognized as tunable green solvents, show significant potential for enhancing enzyme activity in biocatalytic applications. This study investigated the effect of choline chloride–ethylene glycol DES on lipase Aspergillus niger C2J6 whole cells, employing a self-isolated endophytic strain. By varying molar ratio (1:2–1:4) and water content (0–80%), highest (142.31%) was observed at 1:1.55 with 46% content. Mathematical models were developed to connect composition key properties, including surface tension, conductivity, density, viscosity, refractive index, polarity, activity. Statistical analysis revealed that among physicochemical properties DES, polarity exhibited most impact enzymatic activity, followed by conductivity. provides valuable insights designing optimized systems improve efficiency precision.

Language: Английский

Citations

0
Protein language model empowered the robust ASR-driven PET hydrolase featured with two PET binding motifs DOI Creative Commons

Yibo Song,

Anni Li,

Haiyang Cui

et al.

Green Carbon, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Critical Role of Water beyond the Media to Maintain Protein Stability and Activity in Hydrated Deep Eutectic Solvent DOI
Tanmoy Khan, Nilimesh Das, Suman Bhowmik

et al.

The Journal of Physical Chemistry B, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Hydrated deep eutectic solvents (DESs) are recognized for their potential in biocatalysis due to tunability, biocompatibility, greenness, and ability keep protein stable active. However, the mechanisms governing enzyme stability activity DES remain poorly understood. Herein, using bromelain as model acetamide (0.5)/urea(0.3)/sorbitol(0.2) DES, we provide experimental evidence that modulation of associated water plays a key role dictating hydrated DES. Specifically, rigid at higher concentrations (beyond 40% v/v) stabilizes through entropy but destabilizes it enthalpy. On other hand, flexible dynamics lower result an opposite thermodynamic outcome. Importantly, bulk cannot explain trend, which emphasizes critical near surface. Strikingly, also correlates strongly with bromelain's proteolytic activity. An increasing flexibility leads enhancement This is first study experimentally link behavior offering insights could guide future developments solvent engineering catalysis.

Language: Английский

Citations

1
Unravelling the stabilization mechanism of mono-, di and tri-cholinium citrate-ethylene glycol DESs towards α-chymotrypsin for preservation and activation of the enzyme DOI

Bindu Yadav,

Niketa Yadav,

Pannuru Venkatesu

et al.

Physical Chemistry Chemical Physics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

The present study demonstrates the biocompatibility of new green solvents to achieve increased stability, activity and prolonged storage α-CT.

Language: Английский

Citations

0