Stress-actuated Flexible Microelectrode Arrays for Activity Recording in 3D Neuronal Cultures DOI Creative Commons
João Serra, José C. Mateus, Susana Cardoso

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

ABSTRACT Microelectrode arrays (MEAs) are instrumental in monitoring electrogenic cell populations, such as neuronal cultures, allowing high precision measurements of electrical activity. Although three-dimensional cultures replicate the behavior vivo systems better than two-dimensional models, conventional planar MEAs not well suited to capture activity within networks. Novel MEA geometries can overcome this difficulty, but often at cost increased fabrication complexity. Here, we used stress mismatch between thin film layers fabricate with vertical electrodes, using methods compatible established microfabrication protocols. A micrometric SiO 2 hinge enables control over bending angle flexible polyimide structures embedded electrodes. The performance patterned electrodes was assessed before and after actuation, through impedance measurements, voltage noise mapping, recordings. 3D 30×30 µm showed an 0.96 ± 0.07 MΩ per electrode detected spikes amplitudes 400 µV. These results demonstrate potential developed provide a scalable approach MEAs, enabling enhanced recording capabilities for vitro cultures.

Language: Английский

Brain-on-a-chip: an emerging platform for studying the nanotechnology-biology interface for neurodegenerative disorders DOI Creative Commons
Raquel O. Rodrigues,

Su-Ryon Shin,

Manuel Bañobre‐López

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Sept. 18, 2024

Language: Английский

Citations

9
Advancing Brain Organoid Electrophysiology: Minimally Invasive Technologies for Comprehensive Characterization DOI Creative Commons
Mujeeb Yousuf, Jean‐Christophe Rochet, Pushpapraj Singh

et al.

Advanced Materials Technologies, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Human brain organoids, which originate from pluripotent stem cells, serve as valuable tools for a wide range of research endeavors, replicating function. Their capacity to replicate cellular interactions, morphology, and division provides invaluable insights into development, disease modeling, drug screening. However, conventional morphological analysis methods are often invasive lack real‐time monitoring capabilities, posing limitations achieving comprehensive understanding. Therefore, advancing the comprehension organoid electrophysiology necessitates development minimally measurement technologies with long‐term, high‐resolution capabilities. This review highlights significance human organoids emphasizes need electrophysiological characterization. It delves assessment methods, particularly focusing on 3D microelectrode arrays, electrode insertion mechanisms, importance flexible arrays facilitate recordings. Additionally, various sensors tailored monitor properties introduced, enriching understanding their chemical, thermal, mechanical dynamics.

Language: Английский

Citations

1
Bioengineering tools for next-generation neural organoids DOI Creative Commons
Richard O’Laughlin,

F. C. Cheng,

Hongjun Song

et al.

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103011 - 103011

Published: March 24, 2025

Language: Английский

Citations

1
Flexible and stretchable bioelectronics for organoids DOI Creative Commons

Jaeyong Lee,

Jia Liu

Med-X, Journal Year: 2025, Volume and Issue: 3(1)

Published: Feb. 1, 2025

Abstract Organoids have gained significant interest due to their ability recapitulate the structural, molecular, and functional complexity of corresponding organs. While methods been developed characterize benchmark organoid structural molecular properties, capturing development maturation organoids remains challenging. To address this, multifunctional bioelectronics for interfacing with has actively pursued. However, conventional electronics face limitations in achieving recording control across entire three-dimensional (3D) volume a long-term stable manner large morphological cellular composition changes during development. In this review, we first discuss application interfacing. We then focus on flexible stretchable designed create organoid/electronics hybrids chronically interfaces. also review recent advancements charting multimodal cell activities throughout Furthermore, explore integration other characterization modalities comprehensive cells within 3D tissues. Finally, potential integrating artificial intelligence into system through embedded electronics, harnessing biosymbiotic computational systems. These could provide valuable tools characterizing maturation, establishing patient-specific models, developing therapeutic opportunities, exploring novel strategies. Graphical abstract

Language: Английский

Citations

0
3D Spatiotemporal Activation Mapping of Cardiac Organoids Using Conformal Shell Microelectrode Arrays (MEAs) DOI Creative Commons
Deok‐Ho Kim, Soo Jin Choi,

Z. Liu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Abstract Cardiac organoids have emerged as transformative models for investigating cardiogenesis and cardiac diseases. While traditional 2D microelectrode arrays (MEAs) been used to assess the functionality of organoids, they are limited electrophysiological measurements from a single plane do not capture 3D propagation electrical signals. Here, we present programmable, shape-adaptive shell MEA designed map activity across entire surface organoids. These MEAs fabricated on-chip, with tunable dimensions electrode layout, enabling precise encapsulation spherical Using MEAs, generated isochrone maps conduction velocity vectors, revealing speed trajectory signal in spontaneously beating The optical transparency allowed simultaneous calcium imaging, validating pattern. To demonstrate their utility cardiotoxicity screening, monitored changes treated isoproterenol E-4031 over nine days. We anticipate that combined spatiotemporal mapping, can significantly advance development spatially organized structural disease models, high-throughput drug screening platforms.

Language: Английский

Citations

0
Soft 3D Bioelectrodes for Intraorganoid Signal Monitoring in Cardiac Models DOI
Young‐Geun Park, Sumin Kim, Sungjin Min

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Continuous monitoring of physiological activities within the internal regions three-dimensional (3D) organoids holds significant promise for advancing organoid-based research. However, conventional methods are constrained to capturing signals from peripheral surfaces organoids, limiting insights into dynamics. Here, we present a soft 3D bioelectrode platform continuous intraorganoid signal monitoring. These bioelectrodes, formed via printing liquid metal, designed with customizable geometric parameters, including height and diameter, adapt various organoid structures. The tissue-comparable softness electrodes minimizes damage cardiac ensuring stable interface reliable recording even under dynamic deformations caused by rhythmic contractions or displacements in aqueous environments. array configuration enables simultaneous electrocardiogram (ECG) recordings 32 organoids. Demonstrating real-time drug-induced ECG responses, this scalable highlights its potential high-throughput drug screening.

Language: Английский

Citations

0
Closed-loop rehabilitation of upper-limb dyskinesia after stroke: from natural motion to neuronal microfluidics DOI Creative Commons
Hong‐Gang Wang, J. Guo, Yanjie Zhang

et al.

Journal of NeuroEngineering and Rehabilitation, Journal Year: 2025, Volume and Issue: 22(1)

Published: April 19, 2025

This review proposes an innovative closed-loop rehabilitation strategy that integrates multiple subdomains of stroke science to address the global challenge upper-limb dyskinesia post-stroke. Despite advancements in neural remodeling and research, compartmentalization has limited effectiveness current strategies. Our approach unites key areas-including post-stroke brain, robotics, motion sensing, metrics, microfluidics, neuroelectronics-into a cohesive framework designed enhance outcomes. By leveraging cutting-edge technologies such as lightweight advanced microfluidic models, this enables real-time monitoring, adaptive interventions, personalized plans. Furthermore, we explore potential systems drive plasticity functional recovery, offering transformative perspective on rehabilitation. Finally, discuss future directions, emphasizing integration emerging interdisciplinary collaboration advance field. highlights promise strategies achieving unprecedented improving

Language: Английский

Citations

0
Contraindicated Drug Responses in Dravet Syndrome Brain Organoids Utilizing Micro Electrode Array Assessment Methods DOI Creative Commons

Remi Yokoi,

Nami Nagafuku,

Yuto Ishibashi

et al.

Organoids, Journal Year: 2023, Volume and Issue: 2(4), P. 177 - 191

Published: Oct. 26, 2023

Ensuring drug safety for patients with specific neurological disorders is of paramount importance. For instance, certain antiepileptic drugs (AEDs) are contraindicated in Dravet Syndrome (DS), which characterized by a deficiency Na+ channel function. Constructing vitro assessment methods capable detecting responses and medication effects on neurons derived from DS highly anticipated therapeutic innovation. This study used micro electrode array (MEA) measurements low-frequency analysis human iPSC-derived organoids to investigate AED responses. When exposed the carbamazepine phenytoin, number network oscillations increased while maintaining oscillation intensity. Furthermore, administration appeared enhance activities beyond partially consistent findings mouse model. Conversely, treatment sodium valproate resulted similar decrease activity both healthy organoids. The frequency characteristics spontaneous firings AEDs responsiveness demonstrated partial correlation typical electroencephalography patterns observed vivo. In conclusion, this study, employing MEA analysis, revealed disease-specific functional organoids, effective patient assessment, precision medicine, screening.

Language: Английский

Citations

2
Laser-patterned epoxy-based 3D microelectrode arrays for extracellular recording DOI Creative Commons
Peng Hu, Inola Kopic,

Shivani Ratnakar Potfode

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(30), P. 14295 - 14301

Published: Jan. 1, 2024

Epoxy-based 3D microelectrode arrays facilitate extracellular recordings in non-planar environments.

Language: Английский

Citations

0
Microfabricated sensors for non-invasive, real-time monitoring of organoids DOI Creative Commons

Yoojeong Kim,

Erick C. Chica-Carrillo,

Hyunjoo Lee

et al.

Micro and Nano Systems Letters, Journal Year: 2024, Volume and Issue: 12(1)

Published: Dec. 4, 2024

Abstract Organoids are three-dimensional cell clusters derived from stem cells and closely resemble the physiological characteristics of human tissues. As next-generation biological model, organoids provide new opportunities for drug discovery, disease modeling, personalized medicine. To fully harness potential organoids, real-time monitoring states functional evaluation crucial. This review highlights recent advances in real-time, situ biosensing technologies, including microelectrode arrays electrophysiological recordings, chemical sensors biochemical detection, strain mechanical properties. While development miniature non-invasive, long-term, is early stage, these an essential part organoid technology which would insights into developmental biology, pathophysiology, discovery. After reviewing seminal works on microfabricated we also outlook field a discussion remaining challenges future directions with focus integration multiple to facilitate research applications.

Language: Английский

Citations

0