Biomimetics, Journal Year: 2024, Volume and Issue: 9(11), P. 657 - 657
Published: Oct. 27, 2024
Shear stress plays a crucial role in modulating cell adhesion and signaling. We present microfluidic shear generator used to investigate the dynamics of
Language: Английский
Small Science, Journal Year: 2024, Volume and Issue: 4(4)
Published: Feb. 2, 2024
From deciphering infection and disease mechanisms to identifying novel biomarkers personalizing treatments, the characteristics of individual cells can provide significant insights into a variety biological processes facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited terms cost, contamination risks, sample volumes, times, throughput, sensitivity, selectivity. Although microfluidic approaches have been suggested as low‐cost, information‐rich, high‐throughput alternative conventional isolation methods, limitations such necessary off‐chip pre‐ post‐processing well systems designed for workflows restricted their applications. In this review, comprehensive overview recent advances integrated microfluidics on‐chip three prominent application domains provided: investigation somatic (particularly cancer immune cells), stem cells, microorganisms. Also, use cell separation (e.g., dielectrophoresis) unconventional or ways, which advance integration multiple systems, is discussed. Finally, critical discussion related current how they could be overcome provided.
Language: Английский
Citations
8
Applied Spectroscopy, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 11, 2024
We present an attenuated total reflection (ATR) correction scheme capable of rectifying ATR spectra while considering the polarization state for arbitrary angles incidence, provided that this angle exceeds critical entire spectrum. Due to its reliance on weak absorption approximation, it cannot achieve perfect spectra. However, comprehending functionality may offer valuable insights into concept behind approximation. Depending specific instrument accessory combination, outperform proprietary advanced authored by ThermoFisher being as user-friendly, but in contrast latter completely transparent with regard functionality.
Language: Английский
Citations
5
Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: unknown, P. 104716 - 104716
Published: April 1, 2025
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 1, 2025
Modern approaches and technical developments in attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy are leading to new analytical science opportunities. As ATR-FTIR solidifies its foothold as a mature tool, progressively implemented clinical applications, microstructured internal elements (μ-IREs) becoming increasingly important. Fabricated into microgroove structures through anisotropic etching of commercially available silicon wafers, μ-IREs demonstrate both low cost high performance thus gaining popularity across range disciplines alternatives traditional IREs. This review summarizes recent progress applications highlights their fabrication use quantitative electrochemistry. The integration microfluidic devices other systems is discussed with consideration device strategies, along suitability for chemical imaging. Increased commercial availability broadband IR laser sources microscale predicted drive continuing development innovative uses μ-IRE-based label-free diagnostic applications.
Language: Английский
Citations
0
Sensors, Journal Year: 2024, Volume and Issue: 24(5), P. 1529 - 1529
Published: Feb. 27, 2024
Microfluidics has emerged as a robust technology for diverse applications, ranging from bio-medical diagnostics to chemical analysis. Among the different characterization techniques that can be used analyze samples at microfluidic scale, coupling of photonic detection and on-chip configurations is particularly advantageous due its non-invasive nature, which permits sensitive, real-time, high throughput, rapid analyses, taking advantage special environments reduced sample volumes. Putting emphasis on integrated schemes, this review article explores most relevant advances in implementation UV–vis, near-infrared, terahertz, X-ray-based characterizations, punctual spectroscopic or scattering-based measurements types mapping/imaging. The principles their interest are discussed through application systems.
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(42), P. 16609 - 16620
Published: Oct. 12, 2024
In this work, we advance lab-on-chip electrochemistry and spectroscopy by combining these capabilities onto a single platform, thereby achieving mid-infrared spectroelectrochemistry (SEC) for the first time. The key feature of technique is use deterministic laminar flow patterns to precisely transport reacted solution from upstream electrodes downstream spectral detection region. Laminar (LF-SEC) therefore completely new approach, which derives its distinction advantage over traditional SEC physically separating electrode attenuated total reflection (ATR) elements. As such, functional elements retain optimal properties, such as inert, highly conductive bare ATR element sensitive Fourier transform infrared (FTIR) spectroscopy. By ATR-FTIR with scanning aperture system, LF-SEC provides additional spectroscopically monitoring reactions at individual electrodes. system design optimized through series targeted experiments using ferricyanide/ferrocyanide redox pair validate electrochemical functionality, undertake spectroscopic calibration, optimize experimental parameters, finally quantitative relationship between FTIR results reaction rate under galvanostatic control. After optimization, demonstrate oxidation therapeutic compound ascorbic acid (vitamin C) in presence biomolecular interference molecule an overlapping potential. We find that molecular availability causes switch pathways, could finely monitor LF-SEC. This work opens door future developments take microfluidic reactor setup, benefits ranging portability high-throughput studies precise conditions.
Language: Английский
Citations
2
Analytical Methods, Journal Year: 2023, Volume and Issue: 15(39), P. 5129 - 5138
Published: Jan. 1, 2023
Microfluidics has emerged as a powerful technology with diverse applications in microbiology, medicine, chemistry, and physics. While its potential for controlling studying chemical reactions is well recognized, the extraction analysis of useful information generated within microfluidic devices remain challenging. This mainly due to limited tools available situ measurements reactions. In this study, we present proof-of-concept spectIR-fluidic reactor design that combines microfluidics Fourier transform infrared (FTIR) spectroscopy kinetic studies fast By integrating multi-ridge silicon attenuated total reflection (ATR) wafer into device, enable multi-point precise reaction time monitoring. As such, work establishes validated foundation using on-chip ATR-FTIR environment, which enables simultaneous monitoring reagents, intermediates, products phosphate proton transfer reaction. The platform offers customizable designs, allowing investigation various scales, significantly advance exploring mechanisms optimization.
Language: Английский
Citations
6
Published: Nov. 21, 2023
This work addresses the need for kinetic studies to better understand mechanisms that are responsible outputs from flow-based bioelectrochemical systems (BES). Unlike most studies, which focus on electron transport, here we consider reaction kinetics with a chemical mass transport. To achieve this, used precision microfluidic reactor accurately controlled acetate concentration ([Ac]), flow rate (Q), and direction (tangential perpendicular), while output current (I) mature Geobacter sulfurreducens electroactive biofilm (EAB) was measured. A detailed analysis of effects control variables were evaluated over long timeframe, between 1 month nearly year. The results indicate all experimental parameters have outputs, but overall, age is dominant factor. After year, densities as high 29.5 m-1, higher than any other 3-electrode experiment G. EAB. main at early stages appeared be related EAB deacidification, whereas old EAB, increases significantly permeability due structural changes in Additionally, each mode exhibits complementary properties based quantitative apparent enzyme/substrate affinity (KM(app)) maximum (Imax) values. Therefore, addition providing valuable insights biosensors bioelectronics community, these findings open door practical approaches optimization BES device design operation.
Language: Английский
Citations
2
Biomimetics, Journal Year: 2024, Volume and Issue: 9(11), P. 657 - 657
Published: Oct. 27, 2024
Shear stress plays a crucial role in modulating cell adhesion and signaling. We present microfluidic shear generator used to investigate the dynamics of
Language: Английский
Citations
0