Microfluidics for the biological analysis of atmospheric ice-nucleating particles: Perspectives and challenges DOI Creative Commons
Mark D. Tarn, Kirsty J. Shaw, Polly B. Foster

et al.

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

Atmospheric ice-nucleating particles (INPs) make up a vanishingly small proportion of atmospheric aerosol but are key to triggering the freezing supercooled liquid water droplets, altering lifetime and radiative properties clouds having substantial impact on weather climate. However, INPs notoriously difficult model due lack information their global sources, sinks, concentrations, activity, necessitating development new instrumentation for quantifying characterizing in rapid automated manner. Microfluidic technology has been increasingly adopted by ice nucleation research groups recent years as means performing droplet analysis INPs, enabling measurement hundreds or thousands droplets per experiment at temperatures down homogeneous water. The potential microfluidics extends far beyond this, with an entire toolbox bioanalytical separation detection techniques developed over 30 medical applications. Such methods could easily be adapted biological biogenic INP revolutionize field, example, identification quantification bacteria fungi. Combined miniaturized sampling techniques, we can envisage deployment microfluidic sample-to-answer platforms automated, user-friendly field that would enable greater understanding seasonal activity. Here, review various components such platform incorporate highlight feasibility, challenges, endeavor, from assays separations bioanalysis.

Language: Английский

Light‐Responsive Materials in Droplet Manipulation for Biochemical Applications DOI Creative Commons
Guangyao Cheng,

Chit Yau Kuan,

Kuan Wen Lou

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 21, 2024

Miniaturized droplets, characterized by well-controlled microenvironments and capability for parallel processing, have significantly advanced the studies on enzymatic evolution, molecular diagnostics, single-cell analysis. However, manipulation of small-sized including moving, merging, trapping targeted droplets complex biochemical assays subsequent analysis, is not trivial remains technically demanding. Among various techniques, light-driven methods stand out as a promising candidate droplet in facile flexible manner, given features contactless interaction, high spatiotemporal resolution, biocompatibility. This review therefore compiles an in-depth discussion governing mechanisms underpinning manipulation. Besides, light-responsive materials, representing core light-matter interaction key character converting light into different forms energy, are particularly assessed this review. Recent advancements materials most notable applications comprehensively archived evaluated. Continuous innovations rational engineering expected to propel development manipulation, equip with enhanced functionality, broaden routine investigations.

Language: Английский

Citations

24
3D printing and artificial intelligence tools for droplet microfluidics: Advances in the generation and analysis of emulsions DOI

Sibilla Orsini,

Marco Lauricella, Andrea Montessori

et al.

Applied Physics Reviews, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 21, 2025

Droplet microfluidics has emerged as highly relevant technology in diverse fields such nanomaterials synthesis, photonics, drug delivery, regenerative medicine, food science, cosmetics, and agriculture. While significant progress been made understanding the fundamental mechanisms underlying droplet generation microchannels fabricating devices to produce droplets with varied functionality high throughput, challenges persist along two important directions. On one side, generalization of numerical results obtained by computational fluid dynamics would be deepen comprehension complex physical phenomena microfluidics, well capability predicting device behavior. Conversely, truly three-dimensional architectures enhance microfluidic platforms terms tailoring enhancing flow properties. Recent advancements artificial intelligence (AI) additive manufacturing (AM) promise unequaled opportunities for simulating behavior, precisely tracking individual droplets, exploring innovative designs. This review provides a comprehensive overview recent applying AI AM microfluidics. The basic properties multiphase flows production are discussed, current fabrication methods related introduced, together their applications. Delving into use technologies topics covered include AI-assisted simulations real-time within systems, AM-fabrication systems. synergistic combination is expected active matter expediting transition toward fully digital

Language: Английский

Citations

4
Recent advances in centrifugal microfluidics for point-of-care testing DOI
Huijuan Yuan,

Zeyu Miao,

Chao Wan

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.

Language: Английский

Citations

3
Integrating machine learning and biosensors in microfluidic devices: A review DOI Creative Commons
Gianni Antonelli, Joanna Filippi, Michele D’Orazio

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 263, P. 116632 - 116632

Published: Aug. 3, 2024

Microfluidic devices are increasingly widespread in the literature, being applied to numerous exciting applications, from chemical research Point-of-Care devices, passing through drug development and clinical scenarios. Setting up these microenvironments, however, introduces necessity of locally controlling variables involved phenomena under investigation. For this reason, literature has deeply explored possibility introducing sensing elements investigate physical quantities biochemical concentration inside microfluidic devices. Biosensors, particularly, well known for their high accuracy, selectivity, responsiveness. However, signals could be challenging interpret must carefully analysed carry out correct information. In addition, proper data analysis been demonstrated even increase biosensors' mentioned qualities. To regard, machine learning algorithms undoubtedly among most suitable approaches undertake job, automatically highlighting biosensor signals' characteristics at best. Interestingly, it was also benefit themselves, a new paradigm that is starting name "intelligent microfluidics", ideally closing benefic interaction disciplines. This review aims demonstrate advantages triad microfluidics-biosensors-machine learning, which still little used but great perspective. After briefly describing single entities, different sections will benefits dual interactions, applications where reviewed employed.

Language: Английский

Citations

12
Microfluidic Technology: A New Strategy for Controllable Synthesis of Metal Nanomaterials DOI Creative Commons
Dongtang Zhang, Shuang Jia, Jin Zhao

et al.

ChemElectroChem, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Abstract Microfluidic technology has exhibited remarkable potential and significance in the precise preparation of multifunctional nanomaterials. Thanks to its small reaction volume superior hydrodynamic control, this emerged as an essential tool for synthesizing nanomaterials with precisely tunable microstructures morphologies. This paper reviews latest advancements controllable synthesis metal nano‐electrocatalysts utilizing microfluidic technology. Firstly, it systematically elucidates fundamental principles distinctive parameter control strategies. Subsequently, delves deeply into mechanism enhancement process nanoparticles environment. Through analysis specific cases, extensive application prospects advantages system have been further elucidated. Finally, summarizes looks forward challenges future development directions that faces nano‐electrocatalysts. review aims provide valuable insights morphology design technological innovation electrocatalysts.

Language: Английский

Citations

1
A comprehensive understanding on droplets DOI
Gang Chen, Guanhua Lin

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: unknown, P. 103490 - 103490

Published: March 1, 2025

Language: Английский

Citations

1
Droplets in open microfluidics: generation, manipulation, and application in cell analysis DOI
Jiaxu Lin, Ying Hou, Qiang Zhang

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review focuses on droplets in open microfluidics, covering their generation, manipulation and application cell analysis. It could serve as a comprehensive guide for readers to understand explore droplet systems.

Language: Английский

Citations

1
Droplet microfluidics: unveiling the hidden complexity of the human microbiome DOI

Yibin Xu,

Zhiyi Wang, Caiming Li

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The human microbiome is vital for health. Droplet microfluidics offers a versatile toolbox research, enabling single-cell sequencing, cultivation, and functional analyses to deepen our understanding drive innovations.

Language: Английский

Citations

1
Unveiling the complex morphologies of sessile droplets on heterogeneous surfaces DOI Creative Commons
Yanchen Wu, Hongmin Zhang, Fei Wang

et al.

Communications Physics, Journal Year: 2025, Volume and Issue: 8(1)

Published: Jan. 24, 2025

Language: Английский

Citations

1
Production of Monodisperse Oil-in-Water Droplets and Polymeric Microspheres Below 20 μm Using a PDMS-Based Step Emulsification Device DOI Creative Commons
Naotomo Tottori, Seungman Choi, Takasi Nisisako

et al.

Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 132 - 132

Published: Jan. 24, 2025

Step emulsification (SE) is renowned for its robustness in generating monodisperse emulsion droplets at arrayed nozzles. However, few studies have explored poly(dimethylsiloxane) (PDMS)-based SE devices producing oil-in-water (O/W) and polymeric microspheres with diameters below 20 µm-materials broad applicability. In this study, we present a PDMS-based microfluidic device designed to achieve goal. Two 264 nozzles each were fabricated, featuring straight triangular nozzle configurations, both height of 4 µm minimum width 10 µm. The rendered hydrophilic via oxygen plasma treatment. A photocurable acrylate monomer served as the dispersed phase, while an aqueous polyvinyl alcohol solution acted continuous phase. produced polydisperse exceeding 30 coefficient-of-variation (CV) values above 10%. contrast, nozzles, opening 38 µm, consistently generated CVs 4%, maximum throughput 0.5 mL h-1. Off-chip photopolymerization these yielded acrylic microspheres. low-cost, disposable, scalable offers significant potential applications spanning from laboratory-scale research industrial-scale particle manufacturing.

Language: Английский

Citations

1