Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: unknown, P. 343569 - 343569
Published: Dec. 1, 2024
Language: Английский
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.
Language: Английский
Citations
0
Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)
Published: March 1, 2025
The presence of circulating tumor cells (CTCs) in the bloodstream patients with cancer offers crucial insights into progression and early diagnosis. In recent research, detection separation CTCs using microfluidic devices have become as a primary point interest, primarily because their cost-effectiveness facile implementation. This study introduces an omega-shaped device designed for label-free rapid enrichment CTCs. Initial investigations employed numerical simulations to optimize geometry enhanced quality. results indicated that configuration featuring seven Omega channels at velocity 0.5 m/s achieved optimal separation, yielding 100% recovery rate 96.8% purity Subsequently, comprising was constructed evaluate impact varying flow velocities on CTC from blood cells. Experimental findings revealed facilitated efficiency. Notably, experimental demonstrated 88.8% 83.7% CTCs, aligning predictions. Furthermore, surrogate modeling explore range cell types aspect ratio parameter. 0.2 within microchannel, resulting superior performance. addition, it observed diameter greater than 20 μm over 97%.
Language: Английский
Citations
0
TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118240 - 118240
Published: March 1, 2025
Citations
0
Physics of Fluids, Journal Year: 2025, Volume and Issue: 37(3)
Published: March 1, 2025
Detecting circulating tumor cells (CTCs) in cancer patients’ blood and distinguishing them from are crucial for early diagnosis controlling therapy effectiveness. Scientists have increasingly turned to microfluidic devices achieve this separation. Various methods exist separating CTCs cells, including the use of inertial forces, which is a well-established technique based on cell size. Although numerous computational experimental investigations been studied circle-base spiral microchannel, effect spiral's shape has not extensively explored during This study introduces square-based microchannel label-free rapid separation CTCs. In addition, geometric parameters [i.e., radius fillet (R), number fillets aspect ratio (AR)] practical (i.e., Reynolds sample sheath flow) investigated. Efficiency purity significant that demonstrate performance microchannel. The results showed at Re = 6 inlet 15 flow with eleven efficiency was 100%, over 90% Furthermore, surrogate optimization employed investigate different sizes (12–30 μm) optimize AR R. square cross section (AR 1) R 150 μm yielded high purity.
Language: Английский
Citations
0
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: March 27, 2025
Monitoring immune cell function is increasingly being recognized as a more relevant biomarker than traditional white blood counts, yet the need for repeated relatively large volumes still continues to pose significant challenge. To overcome this, we developed sample-sparing platform using inertial microfluidics that can process little 10 μL of isolate leukocytes downstream functional analysis. Our isolates with ∼80% purity, >90% in-device recovery, and >95% viability. Neutrophils were our primary focus due their sensitivity external stimuli critical role in responses. isolated through new method did not show inadvertent activation, evidenced by unchanged expression activation markers CD62L CD11b, phenotypes comparable control cells whole blood. We conducted range assays, including phagocytosis, ROS production, NETosis all tests confirming neutrophils maintained functionality after isolation. These assays performed standard laboratory workflows, demonstrating platform's compatibility techniques such flow cytometry culture assays. Furthermore, showed versatility successfully isolating from challenging samples, mouse vena cava or tail vein, well human capillary obtained fingerstick. This adaptability highlights potential this clinical research applications, particularly frequent monitoring cases where sample volume limited.
Language: Английский
Citations
0
Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)
Published: June 17, 2024
Abstract Biomechanical attributes have emerged as novel markers, providing a reliable means to characterize cellular and subcellular fractions. Numerous studies identified correlations between these factors patients’ medical status. However, the absence of thorough overview impedes their applicability in contemporary state-of-the-art therapeutic strategies. In this context, we provide comprehensive analysis dimensions, configuration, rigidity, density, electrical characteristics normal abnormal circulating cells. Subsequently, discussion broadens encompass bioparticles, such extracellular vesicles (EVs) enriched either from blood cells or other tissues. Notably, cell sizes vary significantly, 2 μm for platelets 25 tumor (CTCs), enabling development size-based separation techniques, microfiltration, specific diagnostic applications. Although density is relatively constant among different it allows gradient centrifugation isolate without altering native state. Additionally, variations EV surface charges (-6.3 -45 mV) offer opportunities electrophoretic electrostatic methods. The distinctive mechanical properties cells, compared counterparts, present an exceptional opportunity diverse biotechnological approaches. This review also aims holistic view current understanding popular techniques domain that transcend conventional boundaries, focusing on early harvesting malignant body fluids, designing effective options, targeting, resonating with tissue genetic engineering principles. Graphical provides clear size/shape, stiffness, cellular/noncellular
Language: Английский
Citations
2
Chemical Engineering Science, Journal Year: 2024, Volume and Issue: 300, P. 120575 - 120575
Published: Aug. 3, 2024
Language: Английский
Citations
2
Fundamental Research, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
1
International Journal of Mechanical Sciences, Journal Year: 2024, Volume and Issue: 278, P. 109471 - 109471
Published: June 9, 2024
Language: Английский
Citations
1
Micromachines, Journal Year: 2024, Volume and Issue: 15(7), P. 818 - 818
Published: June 25, 2024
In the field of biomedicine, efficiently and non-invasively isolating target cells has always been one core challenges. Optical fiber tweezers offer precise non-invasive manipulation within a medium can be easily integrated with microfluidic systems. Therefore, this paper investigated mechanism cell using scattering force optical tweezers. We employed flat-ended single-mode to drive sort derived corresponding formula based on T-matrix model. A system for sorting was developed, an optofluidic experimental platform constructed that effectively integrates chips. The chip, featuring expanded cross-channel design, successfully achieved continuous separation yeast (8~10 µm in diameter) polystyrene microspheres (15~20 diameter), efficiency up 86% maintaining viability approximately 90% cells. Compared other systems, does not require labeling achieve at lower cost instrumentation.
Language: Английский
Citations
1