Pharmacogenomics, Journal Year: 2024, Volume and Issue: 25(8-9), P. 407 - 423
Published: June 12, 2024
To assess the accuracy and technical characteristics of
Language: Английский
Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(2)
Published: Jan. 14, 2025
Language: Английский
Citations
1
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Integration of low-frequency electrical impedance and broadband electromagnetic sensing with microfluidic devices enables high-throughput analysis cell size, membrane properties, intracellular characteristics.
Language: Английский
Citations
1
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: May 2, 2025
Over the years, evolution of point-of-care testing (POCT) has been driven by technological advancements in materials, design, and artificial intelligence, as well breakthrough developments wearable technologies. These innovations are shifting diagnostics from centralized medical facilities to individual homes, meeting growing demand for personalized healthcare. This Review explores recent binding-based assay technologies over past two focusing on platforms such traditional flow assays (lateral vertical flow), fully integrated microfluidic devices, biosensor-integrated systems POCT applications. It emphasizes role optical electrochemical detection methods, which essential ensuring sensitivity, specificity, reliability required a POCT. offer advantages including ease use, high diagnostic accuracy, rapid clinical assessment, cost-effectiveness manufacturing consumables. Additionally, highlights current challenges future perspectives delivering healthcare through portable that operate sample-in, result-out basis.
Language: Английский
Citations
0
Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
A post-pandemic perspective on paper-based microfluidics by George Whitesides and collaborators. Sketch generated artificial intelligence using an original photograph courtesy of Volker Steger (Science Photo Library).
Language: Английский
Citations
0
Micromachines, Journal Year: 2025, Volume and Issue: 16(2), P. 156 - 156
Published: Jan. 29, 2025
Point-of-care (POC) testing has revolutionized diagnostics by providing rapid, accessible solutions outside traditional laboratory settings. However, many POC systems lack the sensitivity or multiplexing capability required for complex diseases. This study introduces an LED-based fluorescence reader designed applications, enabling multiplex detection of lupus nephritis (LN) biomarkers using a biomarker microarray (BMA) slide. The integrates LED excitation source, neutral density (ND) filters precise intensity control, and onboard image processing with Gaussian smoothing centroid thresholding to enhance signal localization. Five LN (VSIG4, OPN, VCAM1, ALCAM, TNFRSF1B) were assessed, performance was validated against Genepix laser-based scanner. demonstrated strong correlation coefficients (r = 0.96-0.98) system both standard curves patient samples, achieving robust signal-to-noise ratios reproducibility across all biomarkers. format reduced sample volume allowed simultaneous analysis multiple These results highlight reader's potential bridge gap between laboratory-grade precision accessibility. By combining portability, cost-effectiveness, high analytical performance, this provides practical solution diagnostics, particularly in resource-limited settings, improving feasibility routine monitoring early intervention diseases requiring comprehensive analysis.
Language: Английский
Citations
0
Small, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 13, 2025
Abstract Point‐of‐care (POC) pathogen detection is highly desirable in diverse fields such as infectious disease diagnosis, food safety testing, and environmental monitoring. Herein, the study seeks to address this critical need by developing an automated microfluidic photothermal quantitative polymerase chain reaction (AMP‐qPCR) system a greatly simplified format. A key element of AMP‐qPCR architecture that combines design clockwork‐like, magnetically‐driven multi‐chamber cartridge with use cheap black tape beneath PCR chamber fast photothermal‐responsive engine. This not only enables unprocessed sample be lysed, purified, subjected real‐time fluorescence ultracompact autonomous manner but also eliminates for sophisticated photonic material/device fabrication frequently required performing ultrafast PCR. It shown can accomplish high‐efficient bacterial DNA extraction within 18.5 min, enabling accurate quantification bacteria concentration from 10 8 2 CFU·mL −1 . Furthermore, its practical applicability demonstrated detecting Neisseria gonorrhoeae sexually transmitted infection‐suspected patients using clinical urine cervical swab specimens, exhibiting matched performance benchtop machine. The presented platform enhances availability POC molecular diagnostics on‐site in‐home testing.
Language: Английский
Citations
0
Advanced Sensor and Energy Materials, Journal Year: 2025, Volume and Issue: unknown, P. 100139 - 100139
Published: Feb. 1, 2025
Language: Английский
Citations
0
Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 534, P. 216588 - 216588
Published: March 9, 2025
Language: Английский
Citations
0
Open Life Sciences, Journal Year: 2025, Volume and Issue: 20(1)
Published: Jan. 1, 2025
Abstract Hereditary hearing loss, an auditory neuropathy disorder, is characterized by its high prevalence and significant impact on the quality of life those affected. In Chinese populations, most prevalent gap junction beta-2 ( GJB2 ) mutation hotspot c.235delC. Currently available genetic tests require expensive instruments specialized technicians or have long testing cycles costs, therefore cannot meet point-of-care (POCT) requirements. The objective this study was to evaluate viability a POCT kit. only 42 min, we successfully identified site c.235delC integrating CRISPR-Cas nucleic acid detection with recombinase-aided amplification (RAA) lateral flow dipstick (LFD) method. This method has capacity detect low-abundance acids (as low as 10 2 copies/μL) frequency (20%), in addition accurately distinguishing wild-type, homozygous, heterozygous mutation. approach utilized assess blood samples from total 31 deaf patients 5 healthy volunteers. All results were subsequently confirmed through implementation Sanger sequencing. Our consistent sequencing results. diagnostic sensitivity specificity 100%. combination CRISPR-Cas13a LFD may be promising for deafness genes.
Language: Английский
Citations
0
Microsystems & Nanoengineering, Journal Year: 2024, Volume and Issue: 10(1)
Published: Nov. 29, 2024
Abstract Early and accurate diagnosis of human immunodeficiency virus (HIV) infection is essential for timely initiation antiretroviral therapy (ART) prevention new infections. However, conventional nucleic-acid-based tests HIV detection require sophisticated laboratory equipment trained personnel, which are often unavailable at the point-of-care (POC) or unaffordable in resource-limited settings. We report our development a low-cost, integrated platform POC testing HIV. The integrates viral nucleic acid extraction on paper substrate reverse transcription loop-mediated isothermal amplification (RT-LAMP) portable, battery-powered heating device with real-time detection. does not infrastructure such as power outlets. assay showed limit 30 copies/mL RNA 140 μL serum 4 copies/reaction using 50 serum, no cross-reactivity hepatitis C (HCV). validated both plasma samples spiked clinical from HIV-positive individuals, compared it standard assays based polymerase chain reaction (PCR). These results demonstrate feasibility POC.
Language: Английский
Citations
2