Decoding the molecular pathways governing trophoblast migration and placental development; a literature review DOI Creative Commons
Lianlian Liu, Lin Tang, Daiwen Chen

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 27, 2024

Placental development is a multifaceted process critical for fruitful pregnancy, reinforced by complex network of molecular pathways that synchronize trophoblast migration, differentiation, and overall placental function. This review provides an in-depth analysis the key signaling pathways, such as Wnt, Notch, TGF-β, VEGF, which play fundamental roles in proliferation, invasion, complicated vascular development. For instance, Wnt pathway essential to balance stem cell proliferation while Notch stimulates fate decisions invasive behavior. TGF-β plays role invasion predominantly response low oxygen environment early regulated hypoxia-inducible factors (HIFs). These promote adaptation, ensure proper attachment vascularization, facilitate adequate fetal-maternal exchange. Further, we explore epigenetic post-transcriptional regulatory mechanisms regulate function, including DNA methylation contribution non-coding RNAs, contribute fine-tuning gene expression during Dysregulation these associated with severe pregnancy complications, preeclampsia, intrauterine growth restriction, recurrent miscarriage, emphasizing need targeted therapeutic strategies. Finally, emerging technologies like organoids, single-cell RNA sequencing, placenta-on-chip models are discussed innovative tools hold promise advancing our understanding biology developing novel interventions improve outcomes. emphasizes importance better address dysfunctions disorders.

Language: Английский

The effect of Polybrominated diphenyl ethers at the fetal blood-brain-barrier: evaluation using a microphysiological system DOI Creative Commons
Sourabh Sharma, Manuel S. Vidal, Souvik Paul

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: March 12, 2025

Background Glutamate dysregulation leading to neuronal excitotoxicity and neuroinflammation are associated with neurological disorders, specifically autism spectrum disorders (ASD) in preterm neonates. The lack of physiologically relevant vitro models has limited mechanistic understanding glutamate during pregnancy. This study evaluated the effect environmental pollutant flame retardant, Polybrominated Diphenyl Ethers (PBDE) 99 47, on cell viability, dysregulation, using a microphysiologic system (MPS) human fetal blood-brain-barrier organ chip (FB-OOC). Methods FB-OOC is composed 3-cell culture chambers, connected by microchannels, containing 1) brain microvessel endothelial cells (HBMEC), 2) vascular pericytes (HBVP), 3) triculture neurons, astrocytes, microglia 5:2:1 ratio, respectively. To assess toxicants neuroinflammation, control (standard media) were exposed PBDE 47 (150 ng/mL). mimic passage through placenta, conditioned PDBE media (1:1) derived from placenta-OOC. In parallel, directly treated 96-well plate. Dextran propagation over 72 h confirmed FB barrier function. activation status was determined immunocytochemistry for CD11 b Iba1, Cell morphology (microscopy), cytotoxicity (Lactate Dehydrogenase assays), cytokines (multiplex assay) measured. Results Physiologic FB-OOCs characterized viable cultures expressing standard morphologies cell-specific markers, formation decreased dextran h, baseline pro-inflammatory cytokine production. On-chip placenta-derived metabolites treatment chamber induced significant upregulation but did not induce nor compared controls. Conversely, 2D experiments showed direct treatment-induced (TNF-α, GM-CSF, IL-8) or Conclusion established an model that recreated intercellular interactions. We report PBDE-induced often development ASD, independent neuroinflammation.

Language: Английский

Citations

0
Organ‐on‐Chip: The Future of Nutrition Research in a One Health World DOI Creative Commons
Manuela Cassotta, María Zabaleta, Sandra Cano

et al.

Food Frontiers, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

ABSTRACT The One Health approach emphasizes the interconnectedness of human, animal, and environmental health, recognizing that health each is interdependent influenced by shared ecosystems. Nutrition research plays a critical role in improving outcomes across these domains, with implications for sustainability food security. Organ‐on‐chip (OoC) technologies have emerged as innovative tools replicating key organ functions, supporting disease modeling, drug discovery, personalized medicine. They also hold promise alternatives to traditional animal models. This systematic review examines potential OoC within framework nutrition research, focusing on (1) their ability replicate human (2) applications safety ecotoxicology, (3) use studying components’ effects. Challenges future directions adoption are discussed. Although fully complexity vivo physiology remains challenge, OoCs offer promising platform simulate functions interactions. These systems significant advancing assessments, impacts addressing systems. such standardization, scalability, accessibility, biases toward models remain. Despite hurdles, current advancements underscore versatility OoCs, positioning them valuable driving innovation feed safety, ecotoxicology. With continued progress, poised make contributions goals framework.

Language: Английский

Citations

0
Modeling reproductive and pregnancy-associated tissues using organ-on-chip platforms: challenges, limitations, and the high throughput data frontier DOI Creative Commons

Nina Truong,

Abir Zahra,

Ryan C. V. Lintao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: April 1, 2025

Over the past decade, organ-on-chip technology (microphysiological systems or tissue chips) has reshaped in-vitro physiological and pathological modeling pharmaceutical drug assessment. FDA Modernization Act 2.0 allows for alternatives to animal testing use of appropriate non-animal models/new approach methods (NAMs), such as Organ-on-chips (OC) platforms in silico simulation models, generate pre-clinical trial data regulatory purposes primes microfluidic field have exponential growth coming years. The changes approaches agencies could significantly impact development therapeutics during pregnancy. However, limitations devices molecular biochemical assay shortfalls hinder progress OOC field. This review describes available reproductive pregnancy-related platforms, current methodologies utilized endpoint datasets (e.g., microscopic imaging, immunocytochemistry, real-time polymerase chain reaction, cytokine multiplex analysis). Microfluidic platform limitations, fewer number cells low supernatant volumes restrictions regarding fabrication materials, are described. Novel spatial transcriptomics, imaging cytometry by time flight, exosomes analysis using Exoview) overcome these challenges primed provide biologically relevant clinically translational that can revolutionize modeling, discovery, toxicologic risk engineering adaptations increase throughput (i.e., device arrays) biological advancements improve both needed reach their full potential.

Language: Английский

Citations

0
Analysis of the evolution of placental oxidative stress research from a bibliometric perspective DOI Creative Commons
Ailing Chen,

Meng‐Yuan Tian,

Zhanfu Luo

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 17, 2024

Research on placental oxidative stress is pivotal for comprehending pregnancy-related physiological changes and disease mechanisms. Despite recent advancements, a comprehensive review of current status, hotspots, trends remains challenging. This bibliometric study systematically analyzes the evolution research, offering reference future studies.

Language: Английский

Citations

1
Decoding the molecular pathways governing trophoblast migration and placental development; a literature review DOI Creative Commons
Lianlian Liu, Lin Tang, Daiwen Chen

et al.

Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 27, 2024

Placental development is a multifaceted process critical for fruitful pregnancy, reinforced by complex network of molecular pathways that synchronize trophoblast migration, differentiation, and overall placental function. This review provides an in-depth analysis the key signaling pathways, such as Wnt, Notch, TGF-β, VEGF, which play fundamental roles in proliferation, invasion, complicated vascular development. For instance, Wnt pathway essential to balance stem cell proliferation while Notch stimulates fate decisions invasive behavior. TGF-β plays role invasion predominantly response low oxygen environment early regulated hypoxia-inducible factors (HIFs). These promote adaptation, ensure proper attachment vascularization, facilitate adequate fetal-maternal exchange. Further, we explore epigenetic post-transcriptional regulatory mechanisms regulate function, including DNA methylation contribution non-coding RNAs, contribute fine-tuning gene expression during Dysregulation these associated with severe pregnancy complications, preeclampsia, intrauterine growth restriction, recurrent miscarriage, emphasizing need targeted therapeutic strategies. Finally, emerging technologies like organoids, single-cell RNA sequencing, placenta-on-chip models are discussed innovative tools hold promise advancing our understanding biology developing novel interventions improve outcomes. emphasizes importance better address dysfunctions disorders.

Language: Английский

Citations

1