Microfluidic creep experiment for measuring linear viscoelastic mechanical properties of microparticles in a cross-slot extensional flow device DOI Creative Commons

Sara Ghanbarpour Mamaghani,

Joanna B. Dahl

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 9, 2024

ABSTRACT The micromechanical measurement field has struggled to establish repeatable techniques, likely because the deforming stresses can be complicated and difficult model. Here we demonstrate experimentally ability of cross-slot microfluidic device create a quasi-steady deformation state in agarose hydrogel microparticles replicate traditional uniaxial creep test at microscale relatively high throughput. A recent numerical study by Lu et al. [Lu, Guo, Yu, Sui. J. Fluid Mech. , 2023, 962, A26] showed that viscoelastic capsules flowing through achieve strain near extensional flow stagnation point is equal equilibrium static strain, thereby implying continuous operation accurately capture capsule elastic mechanical behavior addition transient behavior. However, no studies have reported strains for suspended cells or particles, our knowledge. By using large dimension cross-slots relative microparticle diameter, implementation created an region was enough plateau while dwelling point. This will key precisely measuring linear properties small biological objects. performed regime, so analytical model derived elastic-viscoelastic correspondence principle proposed extract from observed particle histories. Particle image velocimetry measurements unperturbed velocity were used determine where particles experienced should applied. throughput this work 1 – 2 achieving per second, though yield increased with particle-centering upstream design features. Finally, provide recommendations applying experiment other biomaterials criteria identify achieved state.

Language: Английский

Transient flow-induced deformation of cancer cells in microchannels: a general computational model and experiments DOI Creative Commons
Ruixin Lu, Junning Li, Zhengxiao Guo

et al.

Biomechanics and Modeling in Mechanobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Language: Английский

Citations

0
Advancing micromixing techniques: the role of surface acoustic waves and fluid–structure interaction in non-newtonian fluids DOI

Vahid Rabiei Faradonbeh,

Soheil Salahshour, Davood Toghraie

et al.

Microfluidics and Nanofluidics, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 4, 2025

Language: Английский

Citations

0
Microfluidic Constriction-Based Mechanoprofiling of Alginate Microgels and Bladder Cancer Cells DOI
Ingrid H. Øvreeide,

Jacob Sturdy,

Renata Szydlak

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Conditions for a microfluidic creep experiment for microparticles using a cross-slot extensional flow device DOI

Sara Ghanbarpour Mamaghani,

Joanna B. Dahl

Biomicrofluidics, Journal Year: 2025, Volume and Issue: 19(2)

Published: March 1, 2025

The micromechanical measurement field has struggled to establish repeatable techniques because the deforming stresses can be difficult model. A recent numerical study [Lu et al., J. Fluid Mech. 962, A26 (2023)] showed that viscoelastic capsules flowing through a cross-slot achieve quasi-steady strain near extensional flow stagnation point is equal equilibrium static strain, thereby implying capsule's elastic behavior captured in continuous device operation. However, no experimental microfluidic studies have reported strains for suspended cells or particles our knowledge. Here, we demonstrate experimentally conditions necessary replicate uniaxial creep test at microscale and relatively high throughput. By using large dimension cross-slots relative microparticle diameter, implementation creates an region enough agarose hydrogel microparticles plateau while dwelling point. This will key accurately precisely measuring properties of small biological objects. We propose analytical mechanical model extract linear from observed particle histories. Particle image velocimetry measurements unperturbed velocity used estimate where experienced might applied property measurements. Finally, provide recommendations applying experiment other biomaterials criteria identify likely achieved state.

Language: Английский

Citations

0
The role of extracellular matrix viscoelasticity in development and disease DOI Creative Commons
Olivia Courbot,

Alberto Elósegui-Artola

npj Biological Physics and Mechanics., Journal Year: 2025, Volume and Issue: 2(1)

Published: April 3, 2025

Abstract For several decades, research has studied the influence of extracellular matrix (ECM) mechanical properties in cell response, primarily emphasising its elasticity as main determinant and tissue behaviour. However, ECM is not purely elastic; it viscoelastic. viscoelasticity now emerged a major regulator collective dynamics. This review highlights recent findings on role development pathology.

Language: Английский

Citations

0
Biophysical assays to test cellular mechanosensing: moving towards high throughput DOI Creative Commons

Marta Cubero-Sarabia,

Anna Maria Kapetanaki,

Massimo Vassalli

et al.

Biophysical Reviews, Journal Year: 2024, Volume and Issue: 16(6), P. 875 - 882

Published: Dec. 1, 2024

Mechanosensitivity is the ability of cells to sense and respond mechanical stimuli. In order do this, are endowed with different components that allow them react a broad range stimuli, such as compression or shear forces, pressure, vibrations. This sensing process, mechanosensing, involved in fundamental physiological mechanisms, stem cell differentiation migration, but it also central development pathogenic states. Here, we review approaches have been proposed quantify mechanosensation living cells, specific focus on methodologies enable higher experimental throughput. aspect crucial fully understand nuances how impacts physiology pathology systems. We will discuss traditional methods for studying mechanosensing at level single particular attention activation mechanosensitive ion channel piezo1. Moreover, present recent attempts push analysis towards

Language: Английский

Citations

2
Microfluidic creep experiment for measuring linear viscoelastic mechanical properties of microparticles in a cross-slot extensional flow device DOI Creative Commons

Sara Ghanbarpour Mamaghani,

Joanna B. Dahl

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 9, 2024

ABSTRACT The micromechanical measurement field has struggled to establish repeatable techniques, likely because the deforming stresses can be complicated and difficult model. Here we demonstrate experimentally ability of cross-slot microfluidic device create a quasi-steady deformation state in agarose hydrogel microparticles replicate traditional uniaxial creep test at microscale relatively high throughput. A recent numerical study by Lu et al. [Lu, Guo, Yu, Sui. J. Fluid Mech. , 2023, 962, A26] showed that viscoelastic capsules flowing through achieve strain near extensional flow stagnation point is equal equilibrium static strain, thereby implying continuous operation accurately capture capsule elastic mechanical behavior addition transient behavior. However, no studies have reported strains for suspended cells or particles, our knowledge. By using large dimension cross-slots relative microparticle diameter, implementation created an region was enough plateau while dwelling point. This will key precisely measuring linear properties small biological objects. performed regime, so analytical model derived elastic-viscoelastic correspondence principle proposed extract from observed particle histories. Particle image velocimetry measurements unperturbed velocity were used determine where particles experienced should applied. throughput this work 1 – 2 achieving per second, though yield increased with particle-centering upstream design features. Finally, provide recommendations applying experiment other biomaterials criteria identify achieved state.

Language: Английский

Citations

0