Reducing Inert Materials for Optimal Cell–Cell and Cell–Matrix Interactions within Microphysiological Systems DOI Creative Commons
Claudia Olaizola‐Rodrigo, Héctor Castro-Abril, Ismael Perisé-Badía

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(5), P. 262 - 262

Published: April 25, 2024

In the pursuit of achieving a more realistic in vitro simulation human biological tissues, microfluidics has emerged as promising technology. Organ-on-a-chip (OoC) devices, product this technology, contain miniature tissues within microfluidic chips, aiming to closely mimic vivo environment. However, notable drawback is presence inert material between compartments, hindering complete contact tissues. Current membranes, often made PDMS or plastic materials, prevent full interaction cell types and nutrients. Furthermore, their non-physiological mechanical properties composition may induce unexpected responses. Therefore, it essential minimize area cells materials while simultaneously maximizing direct matrices different compartments. The main objective work chip preserving proper cellular distribution. Two devices were designed, each with specific focus on cell–matrix cell–cell interactions. first chip, designed increase interactions, incorporates nylon mesh regular pores 150 microns. second minimizes interference from thereby contact. It features an membrane optimized macropores 1 mm diameter for collagen hydrogel deposition. Biological validation both been conducted through implementation migration cell-to-cell assays, well development epithelia, isolated spheroids. This endeavor contributes advancement aimed at enhancing precision relevance simulations biomimetic models.

Language: Английский

Bone and Joint‐on‐Chip Platforms: Construction Strategies and Applications DOI
Chengcheng Du, Jiacheng Liu, Senrui Liu

et al.

Small Methods, Journal Year: 2024, Volume and Issue: unknown

Published: May 19, 2024

Organ-on-a-chip, also known as "tissue chip," is an advanced platform based on microfluidic systems for constructing miniature organ models in vitro. They can replicate the complex physiological and pathological responses of human organs. In recent years, development bone joint-on-chip platforms aims to simulate processes occurring bones joints, including cell-cell interactions, interplay various biochemical factors, effects mechanical stimuli, intricate connections between multiple future, will integrate advantages disciplines, bringing more possibilities exploring disease mechanisms, drug screening, personalized medicine. This review explores construction application Organ-on-a-chip technology joint research, proposes a modular concept, discusses new opportunities future challenges platforms.

Language: Английский

Citations

6
A Novel Multicompartment Barrier‐Free Microfluidic Device Reveals the Impact of Extracellular Matrix Stiffening and Temozolomide on Immune‐Tumor Interactions in Glioblastoma DOI Open Access
Clara Bayona, Claudia Olaizola‐Rodrigo,

Vira Sharko

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract The immune system plays a crucial role in shaping the glioblastoma tumor microenvironment, characterized by its complexity and dynamic interactions. Understanding tumor‐immune crosstalk is essential for advancing cancer research therapeutic development. Here, novel multicompartment, barrier‐free microfluidic device presented that overcomes limitations of existing models enabling direct interactions without physical barriers, preserving natural cell infiltration. This platform supports independent simultaneous culture cells, replicating healthy‐tumoral stroma interface, allows investigating effect matrix stiffness chemotherapy on both populations. findings reveal increased collagen concentration promotes invasiveness while impairing Additionally, temozolomide treatment reduces motility but enhances anti‐tumor responses. These insights highlight critical roles extracellular mechanics progression modulation, establishing this as powerful tool studying glioblastoma‐immune dynamics evaluating strategies.

Language: Английский

Citations

0
Treatment with trypLE before freezing improves thawing integrity and functionality of sheep ovarian tissue DOI Creative Commons

Alicia Marco,

Marta Gargallo,

Jesús Ciriza

et al.

Journal of Ovarian Research, Journal Year: 2025, Volume and Issue: 18(1)

Published: May 3, 2025

To study innovative approaches to ovarian tissue cryopreservation, a critical issue for fertility preservation in pediatric cancer patients. Despite historical attempts, recent advances treatment have underscored the urgent need more effective and reliable cryopreservation methods. Our research aims evaluate if decreasing rigidity of stroma before by investigating pre-treatments with enzymes can enhance quality post-thawing. evaluated use five commonly used disaggregate (trypLE, collagenase, dispase, accutase hyaluronidase) freezing decrease facilitate cryopreservation. Sheep ovaries, high similarity human were as an animal model. Tissue structure, cell proliferation, apoptosis viability assessed after thawing. findings showed that enzymatic trypLE offered immediate benefits post-thawing highest values percentage intact follicles. A was observed thawing culturing samples. The pretreatment damaged severely also lowest values. Ki67-positive follicles stromal cells fresh samples, but only hyaluronidase maintained antral 2 days culture. Besides, all negative caspase-3 thawing, 7 culture primordial apoptotic treatments apart from trypLE. our suggest could provide beneficial approach maintaining functions cryopreserved ovaries Further is needed fully understand their impact optimize protocols this important clinical context.

Language: Английский

Citations

0
Development of an organ-on-chip model for the detection of volatile organic compounds as potential biomarkers of tumour progression DOI Creative Commons
Clara Bayona, Magdalena Wrona, Teodora Ranđelović

et al.

Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 045002 - 045002

Published: June 12, 2024

Abstract Early detection of tumours remains a significant challenge due to their invasive nature and the limitations current monitoring techniques. Liquid biopsies have emerged as minimally diagnostic approach, wherein volatile organic compounds (VOCs) show potential compelling candidates. However, distinguishing tumour-specific VOCs is difficult presence gases from non-tumour tissues environmental factors. Therefore, it essential develop preclinical models that accurately mimic intricate tumour microenvironment induce cellular metabolic changes secretion tumour-associated VOCs. In this study, microfluidic device was used recreate ischaemic environment within solid for tumour-derived The system represents advance in understanding role biomarkers early holds improve patient prognosis; particularly inaccessible rapidly progressing such glioblastoma.

Language: Английский

Citations

1
Reducing Inert Materials for Optimal Cell–Cell and Cell–Matrix Interactions within Microphysiological Systems DOI Creative Commons
Claudia Olaizola‐Rodrigo, Héctor Castro-Abril, Ismael Perisé-Badía

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(5), P. 262 - 262

Published: April 25, 2024

In the pursuit of achieving a more realistic in vitro simulation human biological tissues, microfluidics has emerged as promising technology. Organ-on-a-chip (OoC) devices, product this technology, contain miniature tissues within microfluidic chips, aiming to closely mimic vivo environment. However, notable drawback is presence inert material between compartments, hindering complete contact tissues. Current membranes, often made PDMS or plastic materials, prevent full interaction cell types and nutrients. Furthermore, their non-physiological mechanical properties composition may induce unexpected responses. Therefore, it essential minimize area cells materials while simultaneously maximizing direct matrices different compartments. The main objective work chip preserving proper cellular distribution. Two devices were designed, each with specific focus on cell–matrix cell–cell interactions. first chip, designed increase interactions, incorporates nylon mesh regular pores 150 microns. second minimizes interference from thereby contact. It features an membrane optimized macropores 1 mm diameter for collagen hydrogel deposition. Biological validation both been conducted through implementation migration cell-to-cell assays, well development epithelia, isolated spheroids. This endeavor contributes advancement aimed at enhancing precision relevance simulations biomimetic models.

Language: Английский

Citations

0