Three-Dimensionally Printed Microsystems to Facilitate Flow-Based Study of Cells from Neurovascular Barriers of the Retina

Micromachines, Journal Year: 2024, Volume and Issue: 15(9), P. 1103 - 1103

Published: Aug. 30, 2024

Rapid prototyping has produced accessible manufacturing methods that offer faster and more cost-effective ways to develop microscale systems for cellular testing. Commercial 3D printers are now increasingly adapted soft lithography, where elastomers used in tandem with 3D-printed substrates produce vitro cell assays. Newfound abilities prototype have begun expand fundamental bioengineering research the visual system complement tissue engineering studies reliant upon complex microtechnology. This project printing elastomeric devices examined responses of retinal cells flow. Our experiments fabricated molds using metal milling, resin stereolithography, fused deposition modeling via plastic printing. The were connected flow pumps simulate different conditions phenotypic endothelial neural significant neurovascular barriers retina. results indicated microdevices demonstrated differences survival morphology response external barrier function. Modern technology shows great potential rapid production testing will contribute both our understanding development new therapies. Future incorporate varied stimuli as well extracellular matrices expanded subsets cells.

Language: Английский

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A Microfluidic Multiplex Sorter for Strain Development

Advanced Materials Technologies, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Selecting strains with superior traits from strain improvement strategies is challenging, as it involves navigating the fitness landscape by applying selective pressures that drive variants peaks of to valleys over time. In recent years, screening and selection conducted via droplet microfluidic methods due its high throughput capabilities. However, oft‐used binary strategy, targeting only levels improved traits, may not reflect overall enhancement. A multiplexed sorting method capable an additional threshold sort phenotypic strength reported. The novel approach uses a droplet‐digital sorter screen different volumes droplets using same device design parameters. This used glucoamylase enzyme mutants two activity (medium high) libraries diastatic yeast have been mutated non‐genetically modified techniques. Using multiplex system, medium‐performing enhanced (up 60%) fermentation kinetics in synthetic beverage media, which would missed approach, are identified. strategy efficiently finds without requiring extensive rounds mutations.

Language: Английский

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Enhanced Cell Growth and Astaxanthin Production in Haematococcus lacustris by Mechanostimulation of Seed Cysts

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(22), P. 10434 - 10434

Published: Nov. 13, 2024

The slow growth and complex life cycle of Haematococcus lacustris pose significant challenges for cost-effective astaxanthin production. This study explores the use microfluidic collision treatment to stimulate germination dormant seed cysts, thereby improving photosynthetic cell productivity in H. cultivated well plate flask cultures. flow rate (1.0–3.0 mL/min) number T-junction loops (3–30) were optimized device. Under optimal conditions (a 2.0 mL/min with 10 loops), total density cultures increased by 44.5% compared untreated controls, reaching 28.9 ± × 104 cells/mL after 72 h. In cultures, treated cysts showed a 21% increase 30 d, 0.95 mg/L/d, due higher biomass concentrations, while content per remained constant. However, excessive physical stress at rates loop numbers resulted reduced viability damage. These findings suggest that carefully controlled cyst mechanostimulation can be an effective environmentally friendly strategy biorefining, enabling production multiple bioactive products.

Language: Английский

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On‐Chip Engineered Living Materials as Field‐Deployable Biosensing Laboratories for Multiplexed Detection

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

Abstract Engineered living materials (ELMs) harness engineered cells to fabricate functional with lifelike characteristics, offering unparalleled potential across various fields. Nonetheless, the deployment of ELM‐based biosensors beyond laboratory settings remains challenging. Herin, ELMs are explored as field‐deployable biosensing laboratories on a microfluidic chip (ELMlab‐on‐Chip) for simultaneous detection diverse analytes in field. This approach engages bottom‐up strategy that includes molecular engineering biosensors, construction stimuli‐responsive ELMs, and fabrication an integrated device. Specifically, fine‐tuned sensitivity response by designing chimeric receptors precisely controlling receptor concentration. Integrating ionic covalent cross‐linking strategies manufacturing ensures good substance permeability mechanical robustness. Moreover, is devised tailored orthogonally creating spatially encoded sensor array output detected miniaturized smartphone‐based The ELMlab‐on‐Chip platform has demonstrated its analysis multiple chemicals from single environmental sample under field conditions, effective expedite real‐world application materials.

Language: Английский

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