Three-Dimensionally Printed Microsystems to Facilitate Flow-Based Study of Cells from Neurovascular Barriers of the Retina
Adam B. Leverant,
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Larissa M. Oprysk,
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Alexandra C. Dabrowski
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et al.
Micromachines,
Journal Year:
2024,
Volume and Issue:
15(9), P. 1103 - 1103
Published: Aug. 30, 2024
Rapid
prototyping
has
produced
accessible
manufacturing
methods
that
offer
faster
and
more
cost-effective
ways
to
develop
microscale
systems
for
cellular
testing.
Commercial
3D
printers
are
now
increasingly
adapted
soft
lithography,
where
elastomers
used
in
tandem
with
3D-printed
substrates
produce
vitro
cell
assays.
Newfound
abilities
prototype
have
begun
expand
fundamental
bioengineering
research
the
visual
system
complement
tissue
engineering
studies
reliant
upon
complex
microtechnology.
This
project
printing
elastomeric
devices
examined
responses
of
retinal
cells
flow.
Our
experiments
fabricated
molds
using
metal
milling,
resin
stereolithography,
fused
deposition
modeling
via
plastic
printing.
The
were
connected
flow
pumps
simulate
different
conditions
phenotypic
endothelial
neural
significant
neurovascular
barriers
retina.
results
indicated
microdevices
demonstrated
differences
survival
morphology
response
external
barrier
function.
Modern
technology
shows
great
potential
rapid
production
testing
will
contribute
both
our
understanding
development
new
therapies.
Future
incorporate
varied
stimuli
as
well
extracellular
matrices
expanded
subsets
cells.
Language: Английский
A Microfluidic Multiplex Sorter for Strain Development
Chiara Leal Alves,
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Sebastien Dumont,
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Zhiyang Deng
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et al.
Advanced Materials Technologies,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 11, 2024
Abstract
Selecting
strains
with
superior
traits
from
strain
improvement
strategies
is
challenging,
as
it
involves
navigating
the
fitness
landscape
by
applying
selective
pressures
that
drive
variants
peaks
of
to
valleys
over
time.
In
recent
years,
screening
and
selection
conducted
via
droplet
microfluidic
methods
due
its
high
throughput
capabilities.
However,
oft‐used
binary
strategy,
targeting
only
levels
improved
traits,
may
not
reflect
overall
enhancement.
A
multiplexed
sorting
method
capable
an
additional
threshold
sort
phenotypic
strength
reported.
The
novel
approach
uses
a
droplet‐digital
sorter
screen
different
volumes
droplets
using
same
device
design
parameters.
This
used
glucoamylase
enzyme
mutants
two
activity
(medium
high)
libraries
diastatic
yeast
have
been
mutated
non‐genetically
modified
techniques.
Using
multiplex
system,
medium‐performing
enhanced
(up
60%)
fermentation
kinetics
in
synthetic
beverage
media,
which
would
missed
approach,
are
identified.
strategy
efficiently
finds
without
requiring
extensive
rounds
mutations.
Language: Английский
Enhanced Cell Growth and Astaxanthin Production in Haematococcus lacustris by Mechanostimulation of Seed Cysts
Catherine Christabel,
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Bolam Kim,
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Aditya Lakshmi Narasimhan
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et al.
Applied Sciences,
Journal Year:
2024,
Volume and Issue:
14(22), P. 10434 - 10434
Published: Nov. 13, 2024
The
slow
growth
and
complex
life
cycle
of
Haematococcus
lacustris
pose
significant
challenges
for
cost-effective
astaxanthin
production.
This
study
explores
the
use
microfluidic
collision
treatment
to
stimulate
germination
dormant
seed
cysts,
thereby
improving
photosynthetic
cell
productivity
in
H.
cultivated
well
plate
flask
cultures.
flow
rate
(1.0–3.0
mL/min)
number
T-junction
loops
(3–30)
were
optimized
device.
Under
optimal
conditions
(a
2.0
mL/min
with
10
loops),
total
density
cultures
increased
by
44.5%
compared
untreated
controls,
reaching
28.9
±
×
104
cells/mL
after
72
h.
In
cultures,
treated
cysts
showed
a
21%
increase
30
d,
0.95
mg/L/d,
due
higher
biomass
concentrations,
while
content
per
remained
constant.
However,
excessive
physical
stress
at
rates
loop
numbers
resulted
reduced
viability
damage.
These
findings
suggest
that
carefully
controlled
cyst
mechanostimulation
can
be
an
effective
environmentally
friendly
strategy
biorefining,
enabling
production
multiple
bioactive
products.
Language: Английский
On‐Chip Engineered Living Materials as Field‐Deployable Biosensing Laboratories for Multiplexed Detection
Benfeng Xu,
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Hui Tian,
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Xinrui Li
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et al.
Advanced Functional Materials,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 8, 2024
Abstract
Engineered
living
materials
(ELMs)
harness
engineered
cells
to
fabricate
functional
with
lifelike
characteristics,
offering
unparalleled
potential
across
various
fields.
Nonetheless,
the
deployment
of
ELM‐based
biosensors
beyond
laboratory
settings
remains
challenging.
Herin,
ELMs
are
explored
as
field‐deployable
biosensing
laboratories
on
a
microfluidic
chip
(ELMlab‐on‐Chip)
for
simultaneous
detection
diverse
analytes
in
field.
This
approach
engages
bottom‐up
strategy
that
includes
molecular
engineering
biosensors,
construction
stimuli‐responsive
ELMs,
and
fabrication
an
integrated
device.
Specifically,
fine‐tuned
sensitivity
response
by
designing
chimeric
receptors
precisely
controlling
receptor
concentration.
Integrating
ionic
covalent
cross‐linking
strategies
manufacturing
ensures
good
substance
permeability
mechanical
robustness.
Moreover,
is
devised
tailored
orthogonally
creating
spatially
encoded
sensor
array
output
detected
miniaturized
smartphone‐based
The
ELMlab‐on‐Chip
platform
has
demonstrated
its
analysis
multiple
chemicals
from
single
environmental
sample
under
field
conditions,
effective
expedite
real‐world
application
materials.
Language: Английский