Aging-related changes in the mechanical properties of single cells DOI Creative Commons
Amarnath Singam, Chandrabali Bhattacharya, Seungman Park

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e32974 - e32974

Published: June 1, 2024

Mechanical properties, along with biochemical and molecular play crucial roles in governing cellular function homeostasis. Cellular mechanics are influenced by various factors, including physiological pathological states, making them potential biomarkers for diseases aging. While several methods such as AFM, particle-tracking microrheology, optical tweezers/stretching, magnetic tweezers/twisting cytometry, microfluidics, micropipette aspiration have been widely utilized to measure the mechanical properties of single cells, our understanding how aging affects these remains limited. To fill this knowledge gap, we provide a brief overview commonly used single-cell properties. We then delve into effects on different cell types. Finally, discuss importance studying viscous viscoelastic well induced stressors gain deeper process aging-related diseases.

Language: Английский

Microfluidic Constriction-Based Mechanoprofiling of Alginate Microgels and Bladder Cancer Cells DOI
Ingrid H. Øvreeide,

Jacob Sturdy,

Renata Szydlak

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Revealing Mechanopathology Induced by Dengue NS1 Using Organ Chips and Single-Cell Force Spectroscopy DOI Creative Commons
Huaqi Tang, Tom M. J. Evers, Mehrad Babaei

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Capillary leakage is a hallmark of severe dengue, yet its precise mechanisms remain elusive. Emerging evidence highlights the dengue virus's targeting mechanically active endothelial cells as key contributor to shock syndrome. The viral nonstructural protein 1 (NS1) has been identified central player, disrupting integrity and inducing vascular hyperpermeability independently pro-inflammatory cytokines. This study provides direct assessment NS1-induced pathology by combining single-cell force spectroscopy microvessel-on-a-chip platform. We demonstrate that NS1 significantly alters cell mechanics, reducing stiffness compromising junctional integrity, thereby directly linking these mechanical alterations dysfunction. These findings establish framework for understanding mechano-pathology offer platform developing targeted therapeutic strategies mitigate disease outcomes.

Language: Английский

Citations

0
Aging-related changes in the mechanical properties of single cells DOI Creative Commons
Amarnath Singam, Chandrabali Bhattacharya, Seungman Park

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(12), P. e32974 - e32974

Published: June 1, 2024

Mechanical properties, along with biochemical and molecular play crucial roles in governing cellular function homeostasis. Cellular mechanics are influenced by various factors, including physiological pathological states, making them potential biomarkers for diseases aging. While several methods such as AFM, particle-tracking microrheology, optical tweezers/stretching, magnetic tweezers/twisting cytometry, microfluidics, micropipette aspiration have been widely utilized to measure the mechanical properties of single cells, our understanding how aging affects these remains limited. To fill this knowledge gap, we provide a brief overview commonly used single-cell properties. We then delve into effects on different cell types. Finally, discuss importance studying viscous viscoelastic well induced stressors gain deeper process aging-related diseases.

Language: Английский

Citations

0