The AtHDA6‐AtSK2 module promotes cold tolerance by enhancing shikimate metabolism and antioxidant activity DOI

Jianxun Su,

Yongke Tian,

Shu-Yi Hao

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(3)

Published: May 1, 2025

SUMMARY Low temperature is an environmental factor that significantly impairs the normal development of plants by limiting yield and quality. Although histone deacetylase HDA6 involved in various biological processes, specific molecular mechanisms underlying its response to low temperatures remain unexplored Arabidopsis. In this study, we investigated expression pattern at discovered cold stress‐induced transcriptional activity increased protein level. Freezing experiments demonstrated functions as a positive regulator temperatures. The point mutant axe1‐5 CRISPR‐edited knockout mutants hda6 CR ‐1 ‐2 exhibited sensitivity temperature, while HDA6‐GFP/axe1‐5 complementation line successfully restored cold‐sensitive phenotype mutant. interacted with deacetylated shikimate kinase SK2. Furthermore, enhanced SK2 stability under stress. SK2‐mediated metabolic pathway crucial for synthesis aromatic amino acids, which are essential antioxidant precursors. Metabolomics analysis showed metabolites decreased stress were primarily concentrated acid synthetic pathway. Additionally, sk2 accumulated higher levels superoxide anion but lower substances stress, suggesting may enhance metabolism, downstream synthesis, accumulation stabilizing SK2, thereby improving tolerance. This study elucidated mechanism positively responds low‐temperature identified antifreeze genes . offers valuable genetic resources theoretical support breeding cold‐resistant varieties crop yield.

Language: Английский

Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products DOI Creative Commons
Ryo Yokoyama

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1914)

Published: Sept. 29, 2024

A diverse array of plant aromatic compounds contributes to the tremendous chemical diversity in kingdom that cannot be seen microbes or animals. Such chemodiversity natural products has emerged, occasionally a lineage-specific manner, adopt challenging environmental niches, as various specialized metabolites play indispensable roles development and stress responses (e.g. lignin, phytohormones, pigments defence compounds). These are synthesized from amino acids (AAAs), l -tyrosine, -phenylalanine -tryptophan. While acid metabolism is generally assumed conserved between animals, plants, recent phylogenomic, biochemical metabolomic studies have revealed AAA supports efficient carbon allocation downstream biosynthetic pathways AAA-derived plants. This review showcases intra- inter-kingdom diversification origin committed enzymes involved biosynthesis catabolism their potential application genetic tools for metabolic engineering. I also discuss evolutionary trends expands article part theme issue ‘The evolution metabolism’.

Language: Английский

Citations

4
Bamboo vinegar powder: Unveiling its antioxidant and antifungal efficacy through bioactive compound analysis and mechanistic insights DOI
Yu-Pei Chen, C.-Y. Oliver Chen, Hongtan Wu

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 470, P. 142718 - 142718

Published: Jan. 2, 2025

Language: Английский

Citations

0
Metabolic Profiling and Substrate Utilization of Hericium erinaceus (Lion’s Mane) Mycelia Isolate Using Biolog Phenotype Microarray DOI Creative Commons

Soumaya Boudagga,

Chadlia Hamdi, Henda Mahmoudi

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Abstract Hericium erinaceus, has long been recognized in traditional Chinese medicine for its extensive health benefits. H. erinaceus is notable production of various bioactive compounds. Among these, the terpenoid compounds hericenones and erinacines, derived from fruiting bodies cultured mycelia respectively, have particularly highlighted their ability to stimulate nerve growth factor (NGF) synthesis, contributing mushroom's neuroprotective properties. This study focuses on metabolic characterization mycelium using Biolog Phenotype MicroArray (PM) technology, a high-throughput method analyzing microbial phenotypes. For first time, PMs were applied elucidate comprehensive nutritional utilization profile erinaceus. The involved testing 950 different conditions, including 190 carbon sources, 380 nitrogen 94 biosynthetic pathways, 59 phosphorus 35 sulfur 96 osmotic pressures, pH conditions. results revealed that relatively restricted profile, efficiently utilizing only 23 out mainly simple sugars certain complex carbohydrates like D-Cellobiose, D-Xylose, Arbutin. also demonstrated effective Urea D-glucosamine, was capable growing all sources most with few exceptions. Additionally, exhibited under 65 56 conditions tested. detailed not enhances our understanding erinaceus' requirements capabilities but provides valuable insights into potential applications development functional foods alternative medicines.

Language: Английский

Citations

0
Association Analysis of the Genomic and Functional Characteristics of Halotolerant Glutamicibacter endophyticus J2-5-19 from the Rhizosphere of Suaeda salsa DOI Creative Commons
Longhao Sun, S. S. Sun,

Tianyang Liu

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 208 - 208

Published: Jan. 18, 2025

Halotolerant plant growth-promoting bacteria (HT-PGPB) have attracted considerable attention for their significant potential in mitigating salt stress crops. However, the current exploration and development of HT-PGPB remain insufficient to meet increasing demands agriculture. In this study, an isolated from coastal saline-alkali soil Yellow River Delta was identified as Glutamicibacter endophyticus J2-5-19. The strain capable growing media with up 13% NaCl producing proteases, siderophores, hormone IAA. Under 4‰ stress, inoculation J2-5-19 significantly increased wheat seed germination rate 37.5% 95%, enhanced dry weight maize seedlings by 41.92%, notably improved root systems. Moreover, work presented first whole-genome endophyticus, revealing that G. resisted expelling sodium ions taking potassium through Na+/H+ antiporters uptake proteins, while also accumulating compatible solutes such betaine, proline, trehalose. Additionally, genome contained multiple key genes, including those involved IAA biosynthesis, siderophore production, GABA synthesis. findings provide a theoretical foundation microbial resources specialized inoculants soils.

Language: Английский

Citations

0
Engineered Biosynthesis of Phenol Using Acetate as the Carbon Source in Escherichia coli DOI
Feng Jiao, Yan Zhao,

Zhendi Liu

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Language: Английский

Citations

0
Exploratory Analysis of Free and Glycosidically Bound Volatile Compounds in Australian-Grown and Imported Dragon Fruit: Implications for Industry Standard Development DOI Creative Commons

S. Chen,

Cheng‐Yuan Xu, Muhammad Sohail Mazhar

et al.

Chemosensors, Journal Year: 2025, Volume and Issue: 13(2), P. 70 - 70

Published: Feb. 15, 2025

Dragon fruit (Hylocereus spp.), a globally popular tropical fruit, is highly regarded for its unique sensory attributes and potential health benefits. However, the volatile organic compound (VOC) profiles that define aroma flavor are underexplored. This exploratory study investigates VOC of Australian-grown imported dragon focusing on free volatiles glycosidically bound released through acid enzymatic hydrolysis. The analysis aims to uncover competitive advantages providing scientific foundation establishing industry standards in Australia, where such currently absent. Using gas chromatography–mass spectrometry (GC-MS) statistical as principal component (PCA), this revealed significant differences influenced by variety growing region. Northern Territory-grown white-fleshed (NTW) displayed most diverse profile, with key contributors acetoin, phenylethyl alcohol, prenol, highlighting premium product. Despite regional similarities, Queensland-grown white- (QLDW) red-fleshed (QLDR) exhibited distinct profiles, compounds farnesol linoleic ethyl ester serving distinguishing markers. Overseas (OverseasW) (OverseasR) samples had less complex likely related earlier harvesting postharvest treatments, emphasizing impact practices complexity. Glycosidically were identified latent precursors enhance during ripening processing. research underscores importance profiling potentially assisting Australian enabling differentiation domestic varieties from imports enhancing market competitiveness. As novel study, future should prioritize identification unknown refine methodologies better understand dynamic changes VOCs storage ripening. These findings provide valuable insights optimizing developing support industry’s growth global positioning.

Language: Английский

Citations

0
Aspirin Eugenol Ester Alleviates Energy Metabolism Disorders by Reducing Oxidative Damage and Inflammation in the Livers of Broilers Under High-Stocking-Density Stress DOI Open Access
Cheng Guo, Yi Zhang, Dongying Bai

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1877 - 1877

Published: Feb. 21, 2025

This study aimed to evaluate the effects of aspirin eugenol ester (AEE) on growth performance, oxidative liver damage, inflammation, and metabolomics in broilers under high-stocking-density (HSD) stress. A total 360 were divided into four groups: normal density (ND, 14/m2), high (HD, 22/m2), ND-AEE (ND + 0.01% AEE), HD-AEE (HD AEE). HSD decreased antioxidant capacity, increased malondialdehyde (MDA) levels, elevated expression cyclooxygenase-2 (COX-2) microsomal prostaglandin E synthase-1 (mPGES-1) mRNA, which contributed reduced performance broilers. Specifically, caused abnormalities linoleic acid metabolism, leading levels Prostaglandin E2 (PGE2) Leukotriene B4 (LTB4) synthesis, aggravated lipid impaired ATP production. AEE counteracted decline broiler production induced by enhancing reducing MDA protecting from maintaining mitochondrial phosphorylation. positively regulated metabolism promoting synthesis γ-linolenic phosphatidylcholine, COX-2 mPGES-1. alleviated metabolic imbalance stress enhanced efficiency fatty oxidation, excess accumulation promoted In summary, this provides strong support for dietary addition alleviate energy disorders

Language: Английский

Citations

0
Transcriptome and nutritional composition analysis of stacked transgenic maize with insect resistance and herbicide tolerance DOI Creative Commons
Xiaoxing Yu, Hongyu Gao, Pengfei Wang

et al.

GM crops & food, Journal Year: 2025, Volume and Issue: 16(1), P. 216 - 234

Published: Feb. 27, 2025

The safety assessment of stacked transgenic crops is essential for their commercial cultivation. A crucial element the nutritional evaluation crops. Currently, profiling methods like transcriptome are employed as supplemental analytical tools to find unintended effects In this study, maize ZDRF8×nCX-1 was produced by crossing two events ZDRF8 and nCX-1. This expresses five genes: cry1Ab, cry2Ab g10evo-epsps (from ZDRF8), well cp4 epsps P450-N-Z1 nCX-1). Molecular analysis showed that insertion sites target genes were not changed during stack breeding, effectively expressed at both RNA protein levels in ZDRF8×nCX-1. Target trait exhibits tolerant glyphosate, flazasulfuron MCPA, resistant damage corn borers. Transcriptome revealed gene-stacked did significantly alter profiles compared Nutritional composition grain profile substantially equivalent non-transgenic counterpart. These results suggest hybrid stacking does cause beyond providing intended beneficial traits.

Language: Английский

Citations

0
Shenqi granules enhance recovery from cerebral ischemia-reperfusion injury by modulating tryptophan and tyrosine metabolism and activating NFE2L2/NRF2 DOI

Hai‐Xin Liu,

Mihi Yang, Yuchang Li

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 140, P. 156623 - 156623

Published: March 10, 2025

Language: Английский

Citations

0
Physiological Response of Penicillium chrysogenum to Mimicked Local and Global Perturbations of Substrate and Dissolved Oxygen Gradients at Industrial‐Scale DOI Open Access
Yining Chen, Cees Haringa, Zejian Wang

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

ABSTRACT Industrial‐scale microbial fermentation processes often face limitations in mixing and mass transfer, leading to the formation of environmental gradients within bioreactor. These expose microbes heterogeneous conditions over time space. In this study, we evaluated effects combined substrate dissolved oxygen (DO) on metabolic response Penicillium chrysogenum at an industrial scale. Three representative environments were simulated scale‐down systems: (1) feed inlet (high glucose, low (HGLO): C S > 20 mM, DO < 0.012 mM), (2) aeration oxygen, glucose (HOLG): 0.8 0.2 (3) global environment (periodic 360 s fluctuation cycle with 45 HGLO 75 HOLG conditions). Results showed that prolonged exposure led a complete loss penicillin production capacity, accompanied by significant excretion intracellular metabolites, effect was largely irreversible. While, cells randomly walking under top impeller zone did not lose capacity but signs premature degeneration due increased energy demand. When exposed environment, finely tuned their metabolism periodical manner, nearly 50% productivity. summary, alone cause irreversible effects, large contributed reduced Oxygen gradients, however, only also caused cellular damage. findings provide valuable insights for developing scale‐up criteria strain engineering strategies aimed improving large‐scale culture performance.

Language: Английский

Citations

0