Polyyne production is regulated by the transcriptional regulators PgnC and GacA in Pseudomonas protegens Pf-5

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Polyynes produced by bacteria have promising applications in agriculture and medicine due to their potent antimicrobial activities. Polyyne biosynthetic genes been identified Pseudomonas Burkholderia. However, the molecular mechanisms underlying regulation of polyyne biosynthesis remain largely unknown. In this study, we used a soil bacterium protegens Pf-5, which was recently reported produce called protegenin, as model investigate bacterial production. Our results show that Pf-5 controls production at both pathway-specific level higher global level. Mutation pgnC, transcriptional regulatory gene located cluster, abolished Gene expression analysis revealed PgnC directly activates promoter genes. The also requires regulator GacA. gacA decreased translation PgnC, is consistent with result pgnC leader mRNA bound RsmE, an RNA-binding protein negatively regulated These suggest GacA induces regulator, turn biosynthesis. Additionally, polyyne-producing strain but not polyyne-nonproducing strain, could inhibit broad spectrum including Gram-negative Gram-positive bacteria.IMPORTANCEAntimicrobial metabolites are widely control plant, animal, human pathogens. Although bacteria-derived polyynes antimicrobials for decades, regulate understudied. found activated induced through RsmE protegens. To our knowledge, work first comprehensive study discovered can help us optimize agricultural or medical applications.

Language: Английский

Elucidation of Antimicrobials and Biofilm Inhibitors Derived from a Polyacetylene Core

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5945 - 5945

Published: Dec. 17, 2024

The development of new antibiotics with unique mechanisms action is paramount to combating the growing threat antibiotic resistance. Recently, based on inspiration from natural products, an asymmetrical polyacetylene core structure was examined for its bioactivity and found have differential specificity different bacterial species substituents around conjugated alkyne. This research further probes structural requirements through a systematic synthesis investigation compounds variable carbon chain length, alkynyl subunits, alcohol substitution. Furthermore, examines activity towards inhibition biofilm formation. Overall, several key polyyne been identified in both decreasing viability disrupting pre-formed biofilms. These properties are fight against infections will be helpful agents.

Language: Английский