Freeze-Driven Adsorption of Oligonucleotides with polyA-Anchors on Au@Pt Nanozyme DOI Open Access

Nikita E. Lapshinov,

С. М. Придворова,

Anatoly V. Zherdev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10108 - 10108

Published: Sept. 20, 2024

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation remarkable catalytic properties. This study aimed explore the freeze–thaw method functionalizing with oligonucleotides featuring a polyadenine anchor. Spherical gold nanoparticles ([Au]NPs) were synthesized subsequently used as seeds produce urchin-like Au@Pt ([Au@Pt]NPs) an average diameter 29.8 nm. The conjugated series non-thiolated DNA oligonucleotides, each consisting three parts: 5′-polyadenine anchor (An, n = 3, 5, 7, 10; triple-branched A3, or A5), random sequence 23 nucleotides, linear polyT block seven deoxythymine residues. resulting conjugates characterized using transmission electron microscopy, spectroscopy, dynamic light scattering, emission detection fluorescent label at 3′-end oligonucleotide. stability was found depend on type oligonucleotide, decreased in row [Au@Pt]NP A7 > A5 3A3 3A5 A10 A3 anchors. These [Au@Pt]NP–oligonucleotide further evaluated lateral flow test strips assess fluorescein-specific binding peroxidase-like activity. Conjugates A5, A7, anchors showed highest levels signals bound labels strips, exceeding sensitivity by up nine times. findings hold significant potential broad application bioanalytical systems.

Language: Английский

Programmable DNA‐Based Nanodevices for Next‐Generation Clinical and Healthcare Applications DOI Open Access
Krupa Kansara,

Anwesha Laha,

Ashutosh Kumar

et al.

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Abstract Deoxyribonucleic acid (DNA) nanotechnology has brought an unparalleled set of possibilities for self‐assembled structures emerging as independent branch synthetic biology. The field science uses the molecular properties DNA to build nanoparticles and nanodevices that have potential bring breakthroughs in medical science. On one hand, their biocompatibility, precision, ease, programmability make them ideal choice drug delivery healthcare. other, lack proper biodistribution profiles, stability inside system, enzymatic cleavage, immune recognition, translational barriers are some hurdles it faces. Many recent technological advancements progress tackle these challenges, while already been used. These tools technologies need be understood studied successful transition intelligent nanostructures (DNs) healthcare applications. This review thus, highlights challenges being faced by DNs Additionally, provides overview trends using devices disease detection remission finally talks about future scope opportunities effective from bench bedside.

Language: Английский

Citations

1
DNA Nanostructures‐Based In Situ Cancer Vaccines: Mechanisms and Applications DOI Open Access
Bingyu Lin, Yanfei Liu, Qi‐Wen Chen

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Abstract Current tumor vaccines suffer from inadequate immune responsive due to the insufficient release of antigens, low infiltration, and immunosuppressive microenvironment. DNA nanostructures with their ability precisely engineer, controlled release, biocompatibility, capability augment immunogenicity microenvironment, have gained significant attention for potential revolutionize vaccine designing. This review summarizes various applications in construction situ cancer vaccines, which can generate tumor‐associated antigens directly damaged tumors immune‐stimulation. The mechanisms components are listed, specific strategies constructing using explored underlying action elucidated. immunogenic cell death (ICD) induced by chemotherapeutic agents, photothermal therapy (PTT), photodynamic (PDT), radiation (RT) related building systematically summarized. different immunotherapy elaborated, exerts precise, long‐lasting, robust responses. current challenges future prospectives proposed. provides a holistic understanding evolving role development.

Language: Английский

Citations

0
The nucleic acid reactions on the nanomaterials surface for biomedicine DOI Creative Commons

Zhenrui Xue,

Lu Wang, Shengnan Pan

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 23, 2025

Integrating nucleic acids (NAs) with nanomaterials has substantially advanced biomedical research, enabling critical applications in biosensing, drug delivery, therapeutics, and the synthesis of nanomaterials. At core these advances are reactions NAs on nanomaterial surfaces, encompassing conjugation (covalent non-covalent), detachment (physical chemical), signal amplification (enzyme-mediated amplification, enzyme-free DNA Walker). Here, we review fundamental mechanisms recent progress acid discuss emerging for diagnostics, nanomedicine, gene therapy, explore persistent challenges field. We offer a forward-looking perspective how future developments could better control, optimize, harness transformative nanomedicine engineering.

Language: Английский

Citations

0
A novel sensitization strategy for uncertain single stranded probe on electrode surface based on terminal deoxynucleotidyl transferase and G-rich spherical nucleic acids DOI
Haoyu Wang, Shiting Chen, Jiqing Liu

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 155899 - 155899

Published: Sept. 1, 2024

Language: Английский

Citations

3
A toolbox for enzymatic modification of nucleic acids with photosensitizers for photodynamic therapy DOI Creative Commons
Germain Niogret,

Camille Chériaux,

Frédéric Bonhomme

et al.

RSC Chemical Biology, Journal Year: 2024, Volume and Issue: 5(9), P. 841 - 852

Published: Jan. 1, 2024

Photodynamic therapy (PDT) is an approved cancer treatment modality. Despite its high efficiency, PDT limited in terms of specificity and by the poor solubility rather lipophilic photosensitizers (PSs). In order to alleviate these limitations, PSs can be conjugated oligonucleotides. However, most conjugation methods often involve complex organic synthesis result appendage single modifications at 3'/5' termini Here, we have investigated possibility bioconjugating a range known polymerase-mediated synthesis. We prepared modified nucleoside triphosphates different substrate tolerance nucleotides for template-dependent -independent DNA polymerases. This method represents mild versatile approach or multiple onto oligonucleotides useful further improve efficiency treatment.

Language: Английский

Citations

1
Freeze-Driven Adsorption of Oligonucleotides with polyA-Anchors on Au@Pt Nanozyme DOI Open Access

Nikita E. Lapshinov,

С. М. Придворова,

Anatoly V. Zherdev

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10108 - 10108

Published: Sept. 20, 2024

A promising and sought-after class of nanozymes for various applications is Pt-containing nanozymes, primarily Au@Pt, due to their easy preparation remarkable catalytic properties. This study aimed explore the freeze–thaw method functionalizing with oligonucleotides featuring a polyadenine anchor. Spherical gold nanoparticles ([Au]NPs) were synthesized subsequently used as seeds produce urchin-like Au@Pt ([Au@Pt]NPs) an average diameter 29.8 nm. The conjugated series non-thiolated DNA oligonucleotides, each consisting three parts: 5′-polyadenine anchor (An, n = 3, 5, 7, 10; triple-branched A3, or A5), random sequence 23 nucleotides, linear polyT block seven deoxythymine residues. resulting conjugates characterized using transmission electron microscopy, spectroscopy, dynamic light scattering, emission detection fluorescent label at 3′-end oligonucleotide. stability was found depend on type oligonucleotide, decreased in row [Au@Pt]NP A7 > A5 3A3 3A5 A10 A3 anchors. These [Au@Pt]NP–oligonucleotide further evaluated lateral flow test strips assess fluorescein-specific binding peroxidase-like activity. Conjugates A5, A7, anchors showed highest levels signals bound labels strips, exceeding sensitivity by up nine times. findings hold significant potential broad application bioanalytical systems.

Language: Английский

Citations

0