Efficient Chemoenzymatic Cascade Reaction for One-Pot Enantioselective Synthesis of Chiral Indole Derivatives in Water DOI
Xiaoting Zhao,

Wen-Dian Li,

Yao Yao

et al.

Published: Jan. 1, 2024

Language: Английский

Efficient chemoenzymatic cascade reaction for one-pot enantioselective synthesis of chiral indole derivatives in water DOI
Xiaoting Zhao,

Wen-Dian Li,

Yao Yao

et al.

Molecular Catalysis, Journal Year: 2025, Volume and Issue: 579, P. 115038 - 115038

Published: March 29, 2025

Language: Английский

Citations

0
Simultaneously Enhanced Catalytic Activity and Thermostability of a Baeyer–Villiger Monooxygenase from Oceanicola granulosus by Reshaping the Binding Pocket DOI
Yu Du,

Xiang Lv,

Chenhao Feng

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(28), P. 15778 - 15787

Published: July 1, 2024

Enzymatic oxygenation of various cyclic ketones into lactones via Baeyer-Villiger monooxygenases (BVMOs) could provide a promising route for synthesizing fragrances and pharmaceutical ingredients. However, unsatisfactory catalytic activity thermostability restricted their applications in the food industries. In this study, we successfully improved monooxygenase (

Language: Английский

Citations

3
Reversing the Enantioselectivity of Enzymatic Dynamic Kinetic Asymmetric Transformations in the Synthesis of Fused Lactones DOI

Mingliang Shi,

Yao Yao, Xinyue Fan

et al.

ACS Catalysis, Journal Year: 2024, Volume and Issue: unknown, P. 17480 - 17488

Published: Nov. 12, 2024

The rational design of one ketoreductase into stereocomplementary variants for controlling the stereoselectivity bulky chiral molecules bearing contiguous stereocenters is highly desirable and challenging. Herein, we report protein engineering from Chryseobacterium sp. CA49 (ChKRED20) through targeted mutagenesis only two key residues (Y188 H145) located in enzyme pocket, achieving precise stereocontrol over synthesis tricyclic fused lactones (highest reversing enantioselectivity >99:1 e.r. to <1:99 e.r.). Notably, both kinetic resolution asymmetric reduction (KR-AR) dynamic transformation (DyKAT) were observed this system. In KR-AR process, ChKRED20 exclusively convert (R)- or (S)-keto esters corresponding enantio- diastereoenriched (R,S)- (S,R)-cis-lactones deliver leftover (S)- (R)-keto esters. On contrary, DyKAT unreactive configurations substrates undergo efficient equilibration via an enolization protonation–deprotonation enzymes. Computational studies are also conducted get insight origin enantioselectivity.

Language: Английский

Citations

1
Efficient Chemoenzymatic Cascade Reaction for One-Pot Enantioselective Synthesis of Chiral Indole Derivatives in Water DOI
Xiaoting Zhao,

Wen-Dian Li,

Yao Yao

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0