Dual-responsive near-infrared turn-on fluorescent probe for cancer stem cell-specific visualization DOI Creative Commons
Koji Miki,

Masahiro Oe,

Kanae Suzuki

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(28), P. 6959 - 6967

Published: Jan. 1, 2024

Aldehyde dehydrogenase 1A1 (ALDH1A1) stands out as one of the most reliable intracellular biomarkers for stem cells because it is expressed in both cancer (CSCs) and normal somatic (NSCs). Although several turn-on fluorescent probes ALDH1A1 have been developed to visualize CSCs cells, discrimination from NSCs difficult. We here report an AND-type dual-responsive probe, CHO_βgal, near-infrared fluorescence which can be turned on after responding β-galactosidase. The dual responsiveness enables clearly visualized, whereas are non-emissive microscopy. CSC-positive metastasis model lungs were successfully discriminated ex vivo staining experiments using single-input ALDH1A1-responsive probe failed achieve this owing pronounced false-positive output lung NSCs. In tissue slice experiments, even presence adjacent tissues, peripheral region-specific localization was clear. versatility CHO_βgal holds promise not only a fundamental vitro research tool visualizing but also valuable asset practical diagnosis, significantly contributing assessment malignancy.

Language: Английский

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine DOI

Yang Zhou,

Yuelin Liu,

Fu-kang Gao

et al.

International Journal of Dairy Technology, Journal Year: 2024, Volume and Issue: 77(3), P. 651 - 679

Published: June 17, 2024

β‐Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β‐1,4‐glycosidic bonds. Though the traditional application of has been to catalyse breakdown lactose in dairy products, its extends beyond production lactose‐free products since variants capable facilitating condensation exhibiting galactosyl transferase activity extensively utilised for synthesis prebiotic galacto‐oligosaccharides. This review analyses β‐Galactosidase multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering applications terms whey treatment, biofuel production, dietary product, galacto‐oligosaccharides early detection cellular senescence tumours.

Language: Английский

Citations

7
Recent Advances in Photoswitchable Fluorescent and Colorimetric Probes DOI Creative Commons
Hongjuan Chen,

Zilong Tang,

Yewen Yang

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(11), P. 2521 - 2521

Published: May 27, 2024

In recent years, significant advancements have been made in the research of photoswitchable probes. These probes undergo reversible structural and electronic changes upon light exposure, thus exhibiting vast potential molecular detection, biological imaging, material science, information storage. Through precisely engineered structures, can toggle between “on” “off” states at specific wavelengths, enabling highly sensitive selective detection targeted analytes. This review systematically presents fluorescent colorimetric built on various photoswitches, primarily focusing types involving photoswitching their and/or signal response processes. It begins with an analysis including photophysical properties, photoisomerization photochromic mechanisms, fundamental design concepts for constructing The article then elaborates applications these detecting diverse targets, cations, anions, small molecules, biomacromolecules. Finally, it offers perspectives current state future development aims to provide a clear introduction researchers field guidance application new, efficient

Language: Английский

Citations

3
Harnessing Aggregation‐Induced Emission‐Based Detection Toolbox for Diagnostics of Urogenital Tumors DOI Creative Commons
Wenzhe Chen,

Hantian Guan,

Yongfeng Lu

et al.

Aggregate, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

ABSTRACT Urogenital system tumors include prostate cancer, bladder ovarian and other very common solid tumor diseases with high morbidity mortality. The unique physiological anatomical features of the urogenital render it particularly amenable to application tissue imaging techniques for diagnostic purposes. advancement aggregation‐induced emission (AIE) materials has addressed limitations associated conventional fluorescent that are prone aggregation‐caused quenching. This facilitated development innovative AIE characterized by enhanced photostability, an increased signal‐to‐noise ratio, improved quality. article reviews research progress biosensors in diagnosis tumors. It mainly involves biomarker vitro fluorescence such as uterine which based on biosensors. In addition, a comprehensive description biosensors’ synthesis strategies is provided. includes detailed elucidation platforms intracellular mechanisms basic principles AIE, accompanied presentation quantitative analysis cell results. limitations, challenges suggestions field summarized, prospect prospected. tumors, also provides catalyst exploring characteristics its wide disease diagnosis.

Language: Английский

Citations

0
Excited-State-Altering Ratiometric Fluorescent Probes for the Response of β-Galactosidase in Senescent Cells DOI Creative Commons

Yanan Han,

Lei Dong,

Lu-Lu Sun

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1221 - 1221

Published: March 8, 2025

β-galactosidase (β-Gal) has emerged as a pivotal biomarker for the comprehensive investigation of diseases associated with cellular senescence. The development fluorescent sensor is considerable importance precisely detecting activity and spatial distribution β-Gal. In this study, we developed two excited-state-altering responsive sensors (TF1 TF2) ratiometric detection Two TCF dyes, composed tricyanofuran (TCF) naphthol units, feature electron “pull–push” systems are quenched fluorescence by Upon β-Gal hydrolysis, significant shift in absorption from ca. 475 nm to 630 observed, accompanied emergence signal at 660 nm. enzyme-responsive optical red-shifts attributed excited-state transition intramolecular charge transfer (ICT) state local excited (LE) state, which was confirmed density functional theory (DFT) calculations. Both display exceptional sensitivity selectivity response PBS solution capable tracking within senescent A549 cells. This study introduces framework developing multimodal probes systematically modulating properties, demonstrating their utility senescence studies, diagnostic assay design, therapeutic assessment.

Language: Английский

Citations

0
Dual-mode ratiometric electrochemical and turn-on fluorescence detection of β-galactosidase in serum based on a single probe DOI
Zuying Wang, Yamei Liu, Huan Yang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113601 - 113601

Published: April 1, 2025

Language: Английский

Citations

0
Glycosylation in cancer: mechanisms, diagnostic markers, and therapeutic applications DOI

Zahraa Qusairy,

Miran Rada

Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Language: Английский

Citations

0
Interaction of a Disaccharide, Lactose With Resorcinol‐Based Acridinedione Dyes in Water: A Combined Study Involving Photophysical, Electrochemical, and Theoretical Approach DOI
Seba Merin Vinod, Sangeetha Murugan Sreedevi, Anju Krishnan

et al.

Luminescence, Journal Year: 2025, Volume and Issue: 40(5)

Published: May 1, 2025

ABSTRACT The interaction of lactose with two structurally distinct resorcinol‐based acridinedione ( AD ‐res) dyes accompanied variation in their photophysical properties was carried out aqueous solution. introduction to ADR1 dye hardly influenced the intramolecular charge transfer (ICT) absorption maxima; however, a clear isosbestic point exists between and disaccharide elucidating ground state complex formation. characteristics ADR2 presence are entirely different from those dye. In case dye, fluorescence enhancement (FE) occurs, quenching (FQ) occurs Lactose solution promotes multiple microenvironments that differ hydrogen‐bonding patterns, such formidable lifetime observed. microenvironment is due more water molecules fewer numbers surrounding vice versa, ascertained through studies. Similarly, electrochemical studies, results shift oxidation reduction potentials both R1 dyes. This correlates combined conventional hydrogen bonding (cHB) hydrophobic interactions, supporting excited‐state nature AD‐res

Language: Английский

Citations

0
Dual-responsive near-infrared turn-on fluorescent probe for cancer stem cell-specific visualization DOI Creative Commons
Koji Miki,

Masahiro Oe,

Kanae Suzuki

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(28), P. 6959 - 6967

Published: Jan. 1, 2024

Aldehyde dehydrogenase 1A1 (ALDH1A1) stands out as one of the most reliable intracellular biomarkers for stem cells because it is expressed in both cancer (CSCs) and normal somatic (NSCs). Although several turn-on fluorescent probes ALDH1A1 have been developed to visualize CSCs cells, discrimination from NSCs difficult. We here report an AND-type dual-responsive probe, CHO_βgal, near-infrared fluorescence which can be turned on after responding β-galactosidase. The dual responsiveness enables clearly visualized, whereas are non-emissive microscopy. CSC-positive metastasis model lungs were successfully discriminated ex vivo staining experiments using single-input ALDH1A1-responsive probe failed achieve this owing pronounced false-positive output lung NSCs. In tissue slice experiments, even presence adjacent tissues, peripheral region-specific localization was clear. versatility CHO_βgal holds promise not only a fundamental vitro research tool visualizing but also valuable asset practical diagnosis, significantly contributing assessment malignancy.

Language: Английский

Citations

0