Confinement-enhanced electrochemiluminescence of copper nanoclusters on 3D layered double hydroxide for ultrasensitive detection of GFAP

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 265, P. 116685 - 116685

Published: Aug. 18, 2024

Language: Английский

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Ultrasensitive Electrochemiluminescence Biosensor with ZIF‐67@MXene as an Efficient Co‐Reaction Accelerator and Plasmonic Nanozyme as a Smart Signal Amplification Probe

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Exploring novel electrochemiluminescence (ECL) co‐reaction accelerators to construct ultrasensitive sensing systems is a prominent focus for developing advanced ECL sensors. However, challenges still remain in finding highly efficient and understanding their promoting mechanisms. In this paper, ZIF‐67@MXene nanosheet composites, with conductive in‐plane structure confined‐stable pore/channel, are designed act as high‐efficient achieve significant enhancement the luminol‐H 2 O based system. Mechanism investigation suggests that hydroxyl radicals (·OH) singlet oxygen ( 1 ) can be selectively preferentially generated on due stable absorption of ·OH , leading remarkable efficiency luminol (830%). Finally, by designing plasmonic NH ‐MIL‐88@Pd nanozyme, an “on‐off” switch immunosensor constructed detection prostate‐specific antigen (PSA). Based multiple signal amplification effect, linear range PSA expanded three orders magnitude. The limit also improved from 1.44 × 10 −11 9.1 −13 g mL −1 . This work proposes effective method preparation provides new strategy sensitive cancer markers.

Language: Английский

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Energy-Transfer-Based Dual-Mode PEC-ECL Biosensor for Acetamiprid Analysis Sensitized by Two-Step DNA Circuit Amplification

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Sensitive and accurate determination of acetamiprid is highly desirable for guaranteeing food safety. In this Letter, an energy-transfer-based dual-mode biosensor was developed using zinc-based metal-organic frameworks (Zn-MOFs) acting as both photoelectrochemical (PEC) electrochemiluminescent (ECL) donors Pt@Cu

Language: Английский

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Ruthenium based metal–organic frameworks as annihilation electrochemiluminescence emitters coupled with DNA walker mediated signal amplification for sensitive detection of aflatoxin B1

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159601 - 159601

Published: Jan. 1, 2025

Language: Английский

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Surface Charge Redistribution-Induced Electrochemiluminescence Enhancement of Gold Nanoclusters: The Novel Generation of Efficient Illuminants

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Herein, we developed the agmatine/6-aza-2-thiothymine templated gold nanoclusters (Agm/ATT-Au NCs) as a novel electrochemiluminescence (ECL) illuminant, which exhibited high ECL in annihilation path via newly defined surface charge redistribution-induced enhancement (SCRIE). Impressively, electrochemical redox reaction of Agm/ATT-Au NCs was enhanced owing to boosted electron transfer kinetics illuminant by positively charged Agm-triggered redistribution ATT-Au NCs, resulting an ∼110-fold higher signal than NCs. This work digs deep into electrogenerated process mechanism direct rational design efficient illuminant. Moreover, powerful successfully applied highly sensitive bioassay platform for detecting glial fibrillary acidic protein (GFAP) with detection limit down 27.5 ag/mL, intense organic light-emitting diode (OLED), and high-definition imaging.

Language: Английский

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Spatial Confinement-Enhanced Electrochemiluminescence of Gold Nanoclusters on 3D Porous ZrO₂ Hollow Nanospheres for the Assessment of Acute Myocardial Infarction Protein Markers

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Herein, the gold nanoclusters@three-dimensional (3D) porous ZrO2 hollow nanospheres (Au NCs@ZrO2) with spatial confinement-enhanced electrochemiluminescence (SCE-ECL) were first prepared to fabricate a biosensing platform for ultrasensitive detection of insulin-like growth factor 1 (IGF-1), which was associated cardiovascular disease, malignant tumor, and neuropathic pain. Specifically, confinement Au NCs in 3D microenvironment significantly boosted optical radiation excited because vibration ligand molecules restricted, recombination holes electrons facilitated process enhancing ECL efficiency, resulting 5.1-fold stronger efficiency than NCs. As proof concept, based on NCs@ZrO2 as an emitter orderly localized catalytic hairpin self-assembly (OL-CHA) system signal amplifier, built achieved fast trace determination IGF-1 limit (LOD) down 0.36 fg/mL. Moreover, realized assessment expression acute myocardial infarction (AMI) patients exhibited more prominent accuracy enzyme-linked immunosorbent assay (ELISA). This work proposed neoteric avenue developing highly efficient emitters, presented promising prospect bioanalysis early diagnosis diseases.

Language: Английский

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Programmable Split DNAzyme Modulators via Allosteric Cooperative Activation for mRNA Electrochemiluminescence Biosensing

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 3, 2025

DNAzymes, known for their programmability, stability, and cost-effectiveness, are powerful tools signal transduction in complex biological systems. However, application responding to target effectors is often hindered by limited catalytic efficiency susceptibility unintended activation. Here we propose an allosteric cooperative activation strategy program a split DNAzyme modulator (STATER) that enables sensitive accurate electrochemiluminescence (ECL) biosensing of interleukin-6 (IL-6) mRNA. Our design features STATER leverages DNA tetrahedron as central scaffold, equipped with two pairs T-shaped hairpin probes (TP) helper (HP). Specifically, the TP contains apurinic/apyrimidinic endonuclease 1 (APE1) recognition sites, IL-6 mRNA region, partzyme fragment, while HP corresponding paired fragment. Unlike conventional modulators rely on single effector activation, integrates mechanism, which ensures all preblocked components synergistically activated assembled within confined space, facilitating rapid specific reconstruction DNAzyme's active domain. Furthermore, upon APE1 mRNA, inactive partzymes undergo assembly via toehold exchange displacement reaction, switching cleavage reactivity STATER. This mechanism establishment threshold thereby minimizing nonspecific scenarios. studies demonstrate exhibits outstanding sensitivity selectivity detection using supramolecular gold nanoclusters network-based ECL platform. The biosensor provides linear span from × 10–13 10–7 M, limit low 3.26 10–14 highlighting STATER's potential detecting various analytes

Language: Английский

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The development of electrochemiluminescent probes: Mechanism and application

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113216 - 113216

Published: March 1, 2025

Language: Английский

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Nanoconfinement‐Enhanced Aggregation‐Induced Electrochemiluminescence for Smartphone‐Adopted Imaging Analysis of cTnI

Advanced Functional Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Abstract The domain‐limited catalytic enhancement has opened novel avenues for the advancement of highly efficient aggregation‐induced electrochemiluminescence (AIECL). Herein, a (ECL) sensing platform is constructed through in situ encapsulation emission (AIE) molecules, tetraphenylethylene, within metal‐organic frameworks (NH 2 ‐MIL‐88). These are uniquely enriched with atomically dispersed active sites that serve as nanoconfined co‐reactant accelerators. This innovative design leads to creation an integrated AIE nanoconfinement reactor. Within this reactor, accelerators catalyze co‐reactants into reactive species (RS) situ, facilitating direct interaction molecules spatially confined composite. Density functional theory calculations and electrochemical electron paramagnetic resonance demonstrate ECL attributed localized amplification, where space formed by coordination self‐assembly alters activation energy barriers improves absorption capacity catalyzing K S O 8 SO 4 ·‐ OH ·− . Additionally, utilizing MATLAB‐mediated image technology deep learning algorithms, smartphone‐adopted self‐reporting AIECL imaging system designed achieve accurate intelligent analysis cardiac troponin I (cTnI). method presents advanced strategy enhance local RS concentrations via catalysis. developed offers facile screening cTnI monitoring.

Language: Английский

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Steric Engineering of Perylene Derivative Side Chains To Boost Electrochemiluminescence for High-Performance Bioanalysis

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

Traditional organic planar aromatic emitters often encounter low electrochemiluminescence (ECL) efficiency due to the aggregation-induced quenching (ACQ) induced by π-π stacking, limiting their applicability in bioanalysis. In this work, we proposed a steric engineering strategy mitigate ACQ perylene diimide-bridged β-cyclodextrin aggregates (Pe-CD aggregates), significantly boosting ECL for trace microRNA (miRNA) detection. By incorporation of large volume cyclic (β-CD) at both side chains diimide, Pe-CD molecules were generated and further assembled into robust supramolecular nanoblocks. This modulates intermolecular distances around cores, effectively minimizing nonradiative relaxation. Molecular simulation results demonstrated that distance increased from 3.32 Å 5.21 Å, accompanied 5.8-fold enhancement compared Pe (formed dense stacking molecules). As proof concept, an bioassay platform was developed using as target-enrichment accelerated-3D DNAzyme walkers signal amplifiers detecting miRNA-21, achieving limit detection 9.7 aM. study offers valuable insights designing new expands potential applications technology.

Language: Английский

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