Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing

Bioresources and Bioprocessing, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 21, 2025

Abstract Enzyme deployment is proliferating extensively in industries owing to their environmentally friendly and easily degradable attributes. This article undertakes an exhaustive examination of wild subtilisin enzyme, covering purification, biochemical delineation, analytical techniques, practical implementations. The purification methodology involved partial refinement, anionic exchange, gel filtration chromatography, culminating a factor 3.406, corroborated by SDS-PAGE showcasing molecular weight ~ 42 kDa. Biochemical scrutiny unveiled the enzyme's response, with optimal pH at 9 temperature peak 60 ℃. Various surfactants, metal ions, organic solvents inhibitors exhibited notable efficacy. Substrate specificity kinetics showcased utmost N-Suc-F-A-A-F- p NA, registering K m V max values 0.731 ± 0.5 mM 0.87 × 10 3 U/mg, respectively. Different bioanalytical techniquesproffered insights into structural biophysical facets. Practical applications encompassed goat skin depilation, feather disintegration, blood clot dissolution, exemplifying multifaceted utility. To embark upon elucidation structure–function relationships, three-dimensional model was devised through homology modelling, leveraging existing structures (PDB: 3WHI). Molecular docking score − 8.8 kcal/mol dynamic simulations augmented comprehension interactions NA. research significantly contributes unravelling intricacies underscores potential industrial biomedical prowess. Subtilisin can be explored for its thrombolytic several cardiovascular diseases. It may aid management thrombosis dissolving clots conditions like deep pulmonary embolism, myocardial infarction, ischemic strokes, atherosclerosis breaking down fibrin arterial plaques, thus preventing heart attacks strokes. Graphical

Language: Английский

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Upcycling Food Waste into Biomaterials Applicable to Medical Products

Sustainability, Journal Year: 2024, Volume and Issue: 16(11), P. 4473 - 4473

Published: May 24, 2024

Globally, an estimated 1.3 billion tons of food are wasted each year, according to a report from the Food and Agriculture Organization United Nations. A variety waste streams constantly generate large amounts that end up in landfills. As is left naturally decay landfills, it emits greenhouse gases pollute environment induce climate change. However, most types contain valuable components can be extracted manufacture industrial products. Therefore, instead abandoning harm environment, there alternative upcycle as new raw materials supply source. This review provides comprehensive update on how environmental sustainability improved using diverse sources biomaterials for fabricating medical products, including lignin, cellulose, chitosan, pectin, collagen, hydroxyapatite, biodegradable polymers. The also highlights biochemical technologies applied extracting useful details current advances developing wound dressings nanoparticles tissue engineering drug delivery.

Language: Английский

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Valorization of Fisheries By-products via Enzymatic Protein Hydrolysis: A Review of Operating Conditions, Process Design, and Future Trends

Process Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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Application of Hydroxyapatite Obtained by Different Techniques: Metabolism and Microarchitecture Characteristics (Review)

Sovremennye tehnologii v medicine, Journal Year: 2024, Volume and Issue: 16(6), P. 60 - 60

Published: Dec. 27, 2024

The literature reports on microarchitecture and metabolism characteristics of synthetic hydroxyapatite obtained by different techniques were analyzed. direct relation between production process its was stated to exist. In turn, largely specifies (a number processes related calcium phosphorus metabolism). Therefore, with reference the various microarchitectures, we analyzed relationship material under study immune system cells. Particular emphasis given a recipient's due microarchitecture. review assessed possible participation cell mitochondria in metabolism. There compared findings vivo vitro depending nanoscale morphology. conclusions emphasized necessity for further investigations immunologically mediated intended bone implants, including development research methods deeper understanding properties. demonstrated potential treating defects specified significance studies develop surgery reconstructive medicine.

Language: Английский

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