Proximity Ligation Induced DNAzyme Motor Cooperating with Exponential Amplification Reaction for Accurate Low-Density Lipoprotein (LDL) Detection

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 26, 2025

Low-density lipoprotein (LDL) is intricately associated with numerous clinical conditions, including dyslipidemia and metabolic-associated fatty liver disease (MAFLD), its serum concentration crucial for diagnostic purposes. However, the sensitive accurate analysis of "intact" LDL a significant difficulty, as conventional approaches typically focus solely on detection cholesterol or surface proteins LDL. We developed proximity ligation-induced DNAzyme motor that facilitates an outstanding amplification reaction precise through simultaneous recognition target ApoB cholesterol. This technique direct quantification particles, opposed to assessing content protein inside The elevated efficiency exponential reaction, in conjunction trans-cleavage activity Cas14a1/crRNA complex, low limit 6.12 mg/dL. unique properties proposed method offer advantages selectivity, stability, sensitivity, rendering it extremely appropriate diagnostics MAFLD.

Language: Английский

Immuno‐Rolling Circle Amplification (Immuno‐RCA): Biosensing Strategies, Practical Applications, and Future Perspectives

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

Abstract In the rapidly evolving field of life sciences and biomedicine, detecting low‐abundance biomolecules, ultraweak biosignals presents significant challenges. This has spurred a rapid development analytical techniques aiming for increased sensitivity specificity. These advancements, including signal amplification strategies integration biorecognition events, mark transformative era in bioanalytical precision accuracy. A prominent method among these innovations is immuno‐rolling circle (immuno‐RCA) technology, which effectively combines immunoassays with via RCA. process starts when targeted biomolecule, such as protein or cell, binds to an immobilized antibody probe on substrate. The introduction circular DNA template triggers RCA, leading exponential significantly enhanced intensity, thus target molecule detectable quantifiable even at single‐molecule level. review provides overview biosensing strategy extensive practical applications immuno‐RCA biomarkers. Furthermore, it scrutinizes limitations inherent sensors sets forth expectations their future trajectory. serves valuable reference advancing various domains, diagnostics, biomarker discovery, molecular imaging.

Language: Английский

Biocatalytic fuel-powered dual-wheel molecular machine for molecular diagnosis of cholangiocarcinoma

Microchemical Journal, Journal Year: 2024, Volume and Issue: 205, P. 111233 - 111233

Published: July 17, 2024

Language: Английский