Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform DOI
Y Li,

Fenglei Quan,

Yige Wu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17362 - 17369

Published: Oct. 16, 2024

Quantitative analysis of cell-free RNA (cfRNA) in plasma sample can be used for screening, diagnosing, and prognosticating multiple diseases. Here, we report a quantitative CRISPR/Cas digital imaging platform (qCasdip) the detection various cfRNAs, including circular RNAs miRNAs, clinical samples at attomolar (aM) level without need preamplification. Digital counting strategy provides qCasdip ability with linear range 10

Language: Английский

Lighting up three-dimensional nanolantern for circular RNA imaging and precise gene therapy DOI

Zheng Shi,

Jinping Hu,

Zichen Jiao

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 276, P. 117273 - 117273

Published: Feb. 15, 2025

Language: Английский

Citations

0
A catalytic hairpin assembly-assisted magnetic SERS platform with core-satellite structure for ultra-sensitive circRNA detection in early lung cancer screening DOI
Luyun Xu,

Yongshi Shen,

Zhizhong Lin

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 163309 - 163309

Published: May 1, 2025

Language: Английский

Citations

0
Novel Approaches in CRISPR/Cas12a-Based Sensing for HCC Diagnosis-A Review (2020-2025) DOI

Cunhong Cen,

Xiyu Liu, Wei He

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: 262, P. 116878 - 116878

Published: April 8, 2025

Language: Английский

Citations

0
Recent Advances in the CRISPR/Cas-Based Nucleic Acid Biosensor for Food Analysis: A Review DOI Creative Commons
Yanan Sun,

Tianjian Wen,

Ping Zhang

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(20), P. 3222 - 3222

Published: Oct. 10, 2024

Food safety is a major public health issue of global concern. In recent years, the CRISPR/Cas system has shown promise in field molecular detection. The been coupled with various nucleic acid amplification methods and combined different signal output systems to develop new generation CRISPR/Cas-based biosensor technology. This review describes design concept its application food analysis. A detailed overview systems, methods, strategies provided. biosensors have advantages high sensitivity, strong specificity, timeliness, achieving fast analysis variety targets, including bacteria, toxins, metal ions, pesticides, veterinary drugs, adulteration, promoting development rapid detection At end, we also provide our outlook for future biosensors.

Language: Английский

Citations

1
Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform DOI
Y Li,

Fenglei Quan,

Yige Wu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17362 - 17369

Published: Oct. 16, 2024

Quantitative analysis of cell-free RNA (cfRNA) in plasma sample can be used for screening, diagnosing, and prognosticating multiple diseases. Here, we report a quantitative CRISPR/Cas digital imaging platform (qCasdip) the detection various cfRNAs, including circular RNAs miRNAs, clinical samples at attomolar (aM) level without need preamplification. Digital counting strategy provides qCasdip ability with linear range 10

Language: Английский

Citations

0